Distribution and characterization of microbial communities in Chrysoperla zastrowi sillemi, an important predator of sap sucking insect pests

Common green lacewing, Chrysoperla zastrowi sillemi is one of the important biological control agents and is used effectively to manage various insect pests. Chrysopid predators are found to harbor many endosymbiotic yeasts and bacteria. Keeping this in view, a study on the distribution of yeast and bacteria in the adult diverticulum and larval gut was conducted using transmission electron microscopy (TEM) and molecular techniques. TEM showed the presence of a load of bacterial cells towards the periphery of inner side of the epithelial lining and the dividing bacterial cells in the larval gut. Numerous oval and kidney shaped yeast fauna were found to be distributed within the lumen and diverticular folds of the diverticulum of adult. Our study reveals the presence and distribution of yeast and bacterial cells from the adult diverticulum and gut of larva. Microbial isolates were identified by sequencing 16S rRNA gene for bacteria and ITS region of yeast including partial rRNA genes ITS-1 and partial 5.8S rRNA gene for yeast, respectively which revealed the presence of yeast isolates namely Kodamaea ohmeri, Torulaspora delbrueckii, Wickerhamomyces anomalus and bacterial isolates namely: Enterobacter hormaechei, E. cloacae and Enterobacter sp. as most common from adult and larvae, respectively.   Key words: Diverticulum, diverticular folds, epithelial lining, lumen, yeast, bacteria

• Publication year

  2014

• Venue

  African Journal of Microbiology Research

• Publication date

  2014-04-02

• Fields of study

  Biology, Environmental Science

• Identifiers
  DOI 10.5897/AJMR2013.6506
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• “This is not an Apple”–Yeast Mutualism in Codling Moth

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  2010cited by this paper
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  2010cited by this paper
• The biology of Chrysopidae and Hemerobiidae (Neuroptera), with reference to their usage as biocontrol agents: a review

  2009cited by this paper
• Occurrence of insecticide resistance in field populations of Chrysoperla zastrowi arabica (Neuroptera:Chrysopidae) in India.

  2009cited by this paper
• Host-symbiont co-speciation and reductive genome evolution in gut symbiotic bacteria of acanthosomatid stinkbugs

  2009cited by this paper
• Population dynamics of defensive symbionts in aphids

  2008cited by this paper
• Evidence of Field-Evolved Resistance to Organophosphates and Pyrethroids in Chrysoperla carnea (Neuroptera: Chrysopidae)

  2008cited by this paper
• A new Sodalis lineage from bloodsucking fly Craterina melbae (Diptera, Hippoboscoidea) originated independently of the tsetse flies symbiont Sodalis glossinidius.

  2007cited by this paper
• Biotype-dependent secondary symbiont communities in sympatric populations of Bemisia tabaci

  2007cited by this paper
• Five novel Candida species in insect-associated yeast clades isolated from Neuroptera and other insects.

  2007cited by this paper
• Metschnikowia noctiluminum sp. nov., Metschnikowia corniflorae sp. nov., and Candida chrysomelidarum sp. nov., isolated from green lacewings and beetles.

  2006cited by this paper
• Observations on Midgut Structure and Content of Chrysoperla carnea (Neuroptera: Chrysopidae)

  2006cited by this paper
• Interspecific Transfer of Bacterial Endosymbionts between Tsetse Fly Species: Infection Establishment and Effect on Host Fitness

  2006cited by this paper
• Evolutionary Relationships of Three New Species of Enterobacteriaceae Living as Symbionts of Aphids and Other Insects

  2005cited by this paper
• The role of yeasts as insect endosymbionts.

  2005cited by this paper
• Aphid Protected from Pathogen by Endosymbiont

  2005cited by this paper
• Microorganisms associated with field-collected Chrysoperla rufilabris (Neuroptera: Chrysopidae) adults with emphasis on yeast symbionts

  2004cited by this paper
• The gut bacteria of insects: nonpathogenic interactions.

  2004influential reference
• Metschnikowia chrysoperlae sp. nov., Candida picachoensis sp. nov. and Candida pimensis sp. nov., isolated from the green lacewings Chrysoperla comanche and Chrysoperla carnea (Neuroptera: Chrysopidae).

  2004cited by this paper
• Morphology of the Alimentary Canal of Chrysoperla rufilabris (Neuroptera: Chrysopidae) Adults in Relation to Microbial Symbionts

  2004influential reference
• Side‐stepping secondary symbionts: widespread horizontal transfer across and beyond the Aphidoidea

  2003cited by this paper
• Effects of three modern insecticides, pyriproxyfen, spinosad and tebufenozide, on survival and reproduction of Chrysoperla carnea adults

  2003cited by this paper
• A polyketide synthase-peptide synthetase gene cluster from an uncultured bacterial symbiont of Paederus beetles

  2002cited by this paper
• Could microbial symbionts of arthropod guts contribute significantly to nitrogen fixation in terrestrial ecosystems?

  2002influential reference
• Nitrogen Fixation by Symbiotic and Free-Living Spirochetes

  2001influential reference
• An aphid-borne bacterium allied to the secondary symbionts of whitefly.

  2001cited by this paper
• Ecology of Prokaryotic Microbes in the Guts of Wood- and Litter-Feeding Termites

  2000influential reference
• Systematic relationships and cospeciation of bacterial endosymbionts and their carpenter ant host species: proposal of the new taxon Candidatus Blochmannia gen. nov.

  2000cited by this paper
• Commercialization of Predators: Recent Lessons from Green Lacewings (Neuroptera: Chrysopidae: Chrosoperla)

  2000cited by this paper
• A simple and rapid method for isolation of high quality genomic DNA from fruit trees and conifers using PVP.

  1997cited by this paper
• Internal bacteria in the Chrysomelidae

  1994cited by this paper
• Novel aspects of the biology of Chrysomelidae

  1994cited by this paper
• 16S rRNA phylogenetic analysis of the bacterial endosymbionts associated with cytoplasmic incompatibility in insects.

  1992cited by this paper
• PCR protocols: a guide to methods and applications.

  1990cited by this paper
• Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics

  1990cited by this paper
• In Situ Production of Hydrolytic Detoxifying Enzymes by Symbiotic Yeasts in the Cigarette Beetle (Coleoptera: Anobiidae)

  1989cited by this paper
• Biology of Chrysopidae

  1986cited by this paper
• 4 – Structure of the Digestive System

  1985cited by this paper
• Yeasts: Characteristics and Identification

  1983cited by this paper
• Biological Insect Pest Suppression

  1977cited by this paper
• Bergey's Manual of Determinative Bacteriology.

  1975cited by this paper
• Some ecophysiological relationships between certain Chrysopa, honeydews and yeasts.

  1970influential reference
• The Origin and Nature of the Adhesive Substance in Larvae of the Genus Chrysopa (Neuroptera: Chrysopidae)

  1962cited by this paper
• Endosymbiose der tiere mit pflanzlichen mikroorganismen

  1953cited by this paper
• Fecundity of Chrysopa californica as affected by synthetic Foods.

  1950cited by this paper
• Bergey's manual of determinative bacteriology

  1926cited by this paper

• Predatory potential and functional responses of Chrysoperla zastrowi sillemi Esben-Peterson (Neuroptera: Chrysopidae) against the red spider mite, Oligonychus coffeae Nietner (Acarina: Tetranychidae), a major pest of tea

  2025cites this paper
• Unveiling the diversity of gut microbes in green lacewings (Chrysopidae: Neuroptera) and their role as protagonist in nutrition

  2025cites this paper
• Seasonal Abundance and Population Dynamics of Chrysoperla spp. in Tomato Agroecosystems

  2025cites this paper
• The insecticides thiamethoxam and chlorantraniliprole do not have side effects on the midgut of the predator Chrysoperla externa (Neuroptera: Chrysopidae)

  2024cites this paper
• Pollens of Fabaceae and Poaceae Alone or Combined with Honey Solution? Suitable Diet for Adults of Chrysoperla externa (Hagen) (Neuroptera: Chrysopidae)

  2024cites this paper
• Biological control potentials of Chrysoperla zastrowi sillemi (Neuroptera: Chrysopidae) against Bemisia tabaci (Hemiptera: Aleyrodidae) on polyhouse grown parthenocarpic cucumber in North-Western India

  2023cites this paper
• Diversity and Functions of Yeast Communities Associated with Insects

  2021cites this paper
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  2021cites this paper
• An Important Afro-Asian Biological Control Agent, Chrysoperla zastrowi sillemi (Neuroptera: Chrysopidae), Invades the New World

  2021cites this paper
• Benefic organisms in agricultural crops: Towards a safety and healthy food in response to COVID-19 and future syndemics

  2021cites this paper
• Anatomy and histology of the alimentary canal of larvae and adults of Chrysoperla externa (Hagen, 1861) (Neuroptera: Chrysopidae).

  2020cites this paper
• Efficacy of the green lace wing, Chrysoperla zastrowi sillemi (Esben-Peterson) (Neuroptera: Chrysopidae), against sucking pests of tomato: an appraisal under protected conditions

  2020cites this paper
• The plant growth-promoting bacterium Kosakonia radicincitans improves fruit yield and quality of Solanum lycopersicum.

  2017cites this paper
• Anatomy and histology of the alimentary canal and ovarioles of Ceraeochrysa cubana adults

  2017cites this paper
• Metabolite Profiling Reveals a Specific Response in Tomato to Predaceous Chrysoperla carnea Larvae and Herbivore(s)-Predator Interactions with the Generalist Pests Tetranychus urticae and Myzus persicae

  2016cites this paper