PURIFICATION OF INTACT MICROTUBULES FROM BRAIN

Hundred-fold purification of intact microtubules from homogenates of rat brain is reported. The success of purification depends on stabilizing the microtubule structure by the combined effects of hexylene glycol, acidic Ph, and low temperature. A practical, negative stain, electron microscopic assay is used to study purity and stability of microtubule fractions. The purified fractions show a major band which migrates like purified tubulin in the SDS gel electrophoresis system.

• Publication year

  1970

• Venue

  Journal of Cell Biology

• Publication date

  1970-11-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1083/JCB.47.2.384PMID 19866738PMCID 2108086
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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