Distinct Domains in High Mobility Group N Variants Modulate Specific Chromatin Modifications*

We have demonstrated that levels of specific modification in histone H3 are modulated by members of the nucleosome-binding high mobility group N (HMGN) protein family in a variant-specific manner. HMGN1 (but not HMGN2) inhibits the phosphorylation of both H3S10 and H3S28, whereas HMGN2 enhances H3K14 acetylation more robustly than HMGN1. Two HMGN domains are necessary for modulating chromatin modifications, a non-modification-specific domain necessary for chromatin binding and a modification-specific domain localized in the C terminus of the HMGNs. Thus, chromatin-binding structural proteins such as HMGNs affect the levels of specific chromatin modifications and therefore may play a role in epigenetic regulation.

• Publication year

  2006

• Venue

  Journal of Biological Chemistry

• Publication date

  2006-04-14

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1074/jbc.M600821200PMID 16484217
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Histone H1 depletion in mammals alters global chromatin structure but causes specific changes in gene regulation.

  2005cited by this paper
• Chromosomal protein HMGN1 enhances the acetylation of lysine 14 in histone H3

  2005cited by this paper
• Chromosomal protein HMGN1 modulates histone H3 phosphorylation.

  2004cited by this paper
• Network of Dynamic Interactions between Histone H1 and High-Mobility-Group Proteins in Chromatin

  2004cited by this paper
• Preparation and functional analysis of HMGN proteins.

  2004cited by this paper
• Protein Phosphatase 2A Activity Affects Histone H3 Phosphorylation and Transcription in Drosophila melanogaster

  2003cited by this paper
• MSK2 and MSK1 mediate the mitogen‐ and stress‐induced phosphorylation of histone H3 and HMG‐14

  2003cited by this paper
• Histone modifications in transcriptional regulation.

  2002cited by this paper
• Translating the Histone Code

  2001cited by this paper
• Chromatin unfolding and activation by HMGN(*) chromosomal proteins.

  2001cited by this paper
• Histone H1 Is a Specific Repressor of Core Histone Acetylation in Chromatin

  2000cited by this paper
• Phosphorylation of histone H3 correlates with transcriptionally active loci.

  2000cited by this paper
• The language of covalent histone modifications

  2000cited by this paper
• The nucleosomal response associated with immediate‐early gene induction is mediated via alternative MAP kinase cascades: MSK1 as a potential histone H3/HMG‐14 kinase

  1999cited by this paper
• Overlapping but Distinct Patterns of Histone Acetylation by the Human Coactivators p300 and PCAF within Nucleosomal Substrates*

  1999cited by this paper
• Roles of histone acetyltransferases and deacetylases in gene regulation

  1998cited by this paper
• Repression by Ume6 involves recruitment of a complex containing Sin3 corepressor and Rpd3 histone deacetylase to target promoters.

  1997cited by this paper
• The HMG-14/-17 chromosomal protein family: architectural elements that enhance transcription from chromatin templates.

  1995cited by this paper
• Homodimers of chromosomal proteins HMG-14 and HMG-17 in nucleosome cores.

  1995cited by this paper
• The cooperative binding of chromosomal protein HMG-14 to nucleosome cores is reduced by single point mutations in the nucleosomal binding domain.

  1994cited by this paper
• The footprint of chromosomal proteins HMG-14 and HMG-17 on chromatin subunits.

  1994cited by this paper
• Use of selectively trypsinized nucleosome core particles to analyze the role of the histone "tails" in the stabilization of the nucleosome.

  1989cited by this paper
• Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension.

  1989cited by this paper

• H3K14: A histone site closely related to diseases

  2025cites this paper
• HMGN1 and HMGN2 are recruited to acetylated and histone variant H2A.Z-containing nucleosomes to regulate chromatin state and transcription

  2025cites this paper
• HMGN2 accelerates the proliferation and cell cycle progression of glioblastoma by regulating CDC20 expression

  2024cites this paper
• Protein intrinsic disorder on a dynamic nucleosomal landscape.

  2021cites this paper
• Prolactin Drives a Dynamic STAT5A/HDAC6/HMGN2 Cis-Regulatory Landscape Exploitable in ER+ Breast Cancer.

  2021cites this paper
• The role of high mobility group of nucleosome binding proteins in stem cell biology and differentiation

  2019influential citation
• Maintenance of active chromatin states by HMGN2 is required for stem cell identity in a pluripotent stem cell model

  2019cites this paper
• Histone Interaction Landscapes Visualized by Crosslinking Mass Spectrometry in Intact Cell Nuclei

  2018cites this paper
• Towards X-ray crystallographic characterization of nucleosome interactions with linker histones, FOXA proteins and high mobility group N factors

  2018cites this paper
• Maintenance of active chromatin states by Hmgn1 and Hmgn2 is required for stem cell identity

  2018cites this paper
• Chapter 9: Epigenetics and Down Syndrome

  2017cites this paper
• Preclinical and Clinical Evidence of DNA Methylation Changes in Response to Trauma and Chronic Stress

  2017cites this paper
• Where Environment Meets Cognition: A Focus on Two Developmental Intellectual Disability Disorders

  2016cites this paper
• Histone H1 and Chromosomal Protein HMGN2 Regulate Prolactin-induced STAT5 Transcription Factor Recruitment and Function in Breast Cancer Cells*

  2016cites this paper
• The role of high mobility nucleosomal binding protein (Hmgn2) in undifferentiated mouse epiblast carcinoma stem cells

  2016cites this paper
• Präklinische Untersuchungen zur Radiopeptidtherapie der Peritonealkarzinose mit Alpha-Strahlern

  2016cites this paper
• Evolution of high mobility group nucleosome-binding proteins and its implications for vertebrate chromatin specialization.

  2015cites this paper
• High mobility group (HMG) proteins: Modulators of chromatin structure and DNA repair in mammalian cells.

  2015influential citation
• Epigenetics: The neglected key to minimize learning and memory deficits in Down syndrome

  2014influential citation
• Chromatin , Gene , and RNA Regulation Loss of the Nucleosome-Binding Protein HMGN 1 Affects the Rate of N-Nitrosodiethylamine-Induced Hepatocarcinogenesis in Mice

  2014cites this paper
• High Mobility Group Nucleosome‐Binding Family Proteins Promote Astrocyte Differentiation of Neural Precursor Cells

  2014cites this paper
• High Mobility Group Nucleosomal Binding Domain 2 (HMGN2) SUMOylation by the SUMO E3 Ligase PIAS1 Decreases the Binding Affinity to Nucleosome Core Particles*

  2014cites this paper
• Genetic and Epigenetic Mechanisms in Down Syndrome Brain

  2013cites this paper
• Structural studies of the nucleosome, assembled with different histones, DNA fragments and HMGN proteins, and derivatized with ruthenium anticancer agents

  2013influential citation
• Epigenetics of stress adaptations in the brain.

  2013cites this paper
• High Mobility Group N Proteins Modulate the Fidelity of the Cellular Transcriptional Profile in a Tissue- and Variant-specific Manner*

  2013cites this paper
• Loss of the Nucleosome-Binding Protein HMGN1 Affects the Rate of N-Nitrosodiethylamine-Induced Hepatocarcinogenesis in Mice

  2013cites this paper
• The chromatin-binding protein HMGN3 stimulates histone acetylation and transcription across the Glyt1 gene.

  2012cites this paper
• Aberrant epigenetic landscape in intellectual disability.

  2012cites this paper
• HMGN2 inducibly binds a novel transactivation domain in nuclear PRLr to coordinate Stat5a-mediated transcription.

  2011cites this paper
• TITLE: The Genomic Actions and Functional Implications of Nuclear PRLr in Human Breast Carcinoma

  2011cites this paper
• The Chromatin-binding Protein HMGN1 Regulates the Expression of Methyl CpG-binding Protein 2 (MECP2) and Affects the Behavior of Mice*

  2011cites this paper
• Barrier-to-Autointegration Factor influences specific histone modifications

  2011cites this paper
• HMGNs, DNA repair and cancer.

  2010cites this paper
• HMG modifications and nuclear function.

  2010cites this paper
• HMGN5/NSBP1: a new member of the HMGN protein family that affects chromatin structure and function.

  2010cites this paper
• Regulation of chromatin structure and function by HMGN proteins.

  2010cites this paper
• Divalent Metal- and High Mobility Group N Protein-Dependent Nucleosome Stability and Conformation

  2010cites this paper
• Signalling to chromatin through post-translational modifications of HMGN.

  2010cites this paper
• The Genomic Actions and Functional Implications of Nuclear PRLr in Human Breast Carcinoma

  2010cites this paper
• HMG Nuclear Proteins: Linking Chromatin Structure to Cellular Phenotype

  2010cites this paper
• Developmental function of HMGN proteins.

  2010cites this paper
• Nuclear functions of the HMG proteins.

  2010cites this paper
• Binding and interplay of HMG proteins on chromatin: lessons from live cell imaging.

  2010influential citation
• The role of the chromatin protein HMGN3 in cytokine induced gene expression

  2009influential citation
• The dynamics of HMG protein-chromatin interactions in living cells.

  2009cites this paper
• The Nucleosome Binding Protein HMGN3 Modulates the Transcription Profile of Pancreatic β Cells and Affects Insulin Secretion

  2009cites this paper
• The Nucleosome Binding Protein HMGN3 Modulates the Transcription Profile of Pancreatic (cid:2) Cells and Affects Insulin Secretion (cid:1)

  2009cites this paper
• Delineation of the Protein Module That Anchors HMGN Proteins to Nucleosomes in the Chromatin of Living Cells

  2008cites this paper
• Chromosomal Protein HMGN1 Enhances the Heat Shock-induced Remodeling of Hsp70 Chromatin*

  2008cites this paper
• Differential expression of the HMGN family of chromatin proteins during ocular development.

  2008cites this paper
• High mobility group proteins and their post-translational modifications.

  2008cites this paper
• In silico modulation of HMGN-1 binding to histones and gene expression by interplay of phosphorylation and O-GlcNAc modification.

  2008cites this paper
• Immunolocalization of the High‐Mobility Group N2 protein and acetylated histone H3K14 in early developing parthenogenetic bovine embryos derived from oocytes of high and low developmental competence

  2008cites this paper
• HMGN1 Modulates Estrogen-Mediated Transcriptional Activation through Interactions with Specific DNA-Binding Transcription Factors

  2007cites this paper
• Chromosomal protein HMGN1 modulates the phosphorylation of serine 1 in histone H2A.

  2006cites this paper