Cloning and Functional Expression of Novel N-type Ca2+ Channel Variants*

Voltage-dependent Ca2+ channels are structurally and functionally diverse. As Ca2+ currents recorded from embryonic chick dorsal root ganglion (DRG) neurons differ significantly from their mammalian counterparts, information on the primary sequence of the chick channels will help define the structural underpinnings of Ca2+ channel function. Here, we report the cloning and functional expression of full-length Ca2+ channel α1B subunit cDNAs derived from chick DRGs. Two variable regions (A and B) have been identified in the cytoplasmic linker between repeats I and II; a third (C) in the carboxyl terminus extends the open reading frame by 525 nucleotides. The A and C inserts are absent, and the B insert is present in all other class B clones reported to date. The unique shorter channels appear to predominate in DRG neurons. Results represent a requisite first step in defining the structural elements that underlie variations in function and modulation of Ca2+ channels.

• Publication year

  1999

• Venue

  Journal of Biological Chemistry

• Publication date

  1999-12-03

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1074/JBC.274.49.34566PMID 10574919
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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