Multi-gene gateway clone design for expression of multiple heterologous genes in living cells: eukaryotic clones containing two and three ORF multi-gene cassettes expressed from a single promoter.

No abstract is available for this paper.

• Publication year

  2005

• Venue

  Journal of Biotechnology

• Publication date

  2005-06-24

• Fields of study

  Biology, Medicine, Engineering

• Identifiers
  DOI 10.1016/j.jbiotec.2008.06.007PMID 18640160
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Multi-gene gateway clone design for expression of multiple heterologous genes in living cells: modular construction of multiple cDNA expression elements using recombinant cloning.

  2005cited by this paper
• Multi-gene gateway clone design for expression of multiple heterologous genes in living cells: conditional gene expression at near physiological levels.

  2005cited by this paper
• Evidence for high specificity and efficiency of multiple recombination signals in mixed DNA cloning by the Multisite Gateway system.

  2004cited by this paper
• Gene organization: selection, selfishness, and serendipity.

  2003cited by this paper
• Transcriptional Interference by Independently Regulated Genes Occurs in Any Relative Arrangement of the Genes and Is Influenced by Chromosomal Integration Position

  2002cited by this paper
• A monomeric red fluorescent protein

  2002cited by this paper
• Subgenomic replicon derived from a cell line infected with the hepatitis C virus.

  2002cited by this paper
• Functional interactions in internal translation initiation directed by viral and cellular IRES elements.

  2001cited by this paper
• Genetically encoded reporters of protein kinase A activity reveal impact of substrate tethering

  2001cited by this paper
• Internal ribosome entry sites in eukaryotic mRNA molecules.

  2001cited by this paper
• A 9-nt segment of a cellular mRNA can function as an internal ribosome entry site (IRES) and when present in linked multiple copies greatly enhances IRES activity.

  2000cited by this paper
• The efficiency of different IRESs (Internal Ribosomes Entry Site) in monocistronic mRNAS

  2000cited by this paper
• Translational upregulation of X-linked inhibitor of apoptosis (XIAP) increases resistance to radiation induced cell death

  2000cited by this paper
• Identification and characterization of a novel cell cycle-regulated internal ribosome entry site.

  2000cited by this paper
• Analysis of the c-myc IRES; a potential role for cell-type specific trans-acting factors and the nuclear compartment.

  2000cited by this paper
• A cell cycle-dependent internal ribosome entry site.

  2000cited by this paper
• DNA cloning using in vitro site-specific recombination.

  2000cited by this paper
• IRES-dependent second gene expression is significantly lower than cap-dependent first gene expression in a bicistronic vector.

  2000cited by this paper
• Internal ribosome entry site biology and its use in expression vectors.

  1999cited by this paper
• Chromosome instability and immunodeficiency syndrome caused by mutations in a DNA methyltransferase gene

  1999cited by this paper
• Synexpression groups in eukaryotes

  1999cited by this paper
• Vertebrate Isoforms of Actin Capping Protein β Have Distinct Functions in Vivo

  1999cited by this paper
• rRNA-complementarity in the 5' untranslated region of mRNA specifying the Gtx homeodomain protein: evidence that base- pairing to 18S rRNA affects translational efficiency.

  1999cited by this paper
• Initiation of translation in prokaryotes and eukaryotes.

  1999cited by this paper
• Transcriptional interference perturbs the binding of Sp1 to the HIV-1 promoter.

  1998cited by this paper
• Vertebrates have conserved capping protein α isoforms with specific expression patterns

  1997cited by this paper
• Genetic analysis of internal ribosomal entry site on hepatitis C virus RNA: implication for involvement of the highly ordered structure and cell type-specific transacting factors.

  1997cited by this paper
• Vertebrates have conserved capping protein alpha isoforms with specific expression patterns.

  1997cited by this paper
• FACS-optimized mutants of the green fluorescent protein (GFP).

  1996cited by this paper
• Internal initiation of translation of hepatitis C virus RNA: the ribosome entry site is at the authentic initiation codon.

  1996cited by this paper
• An enhanced green fluorescent protein allows sensitive detection of gene transfer in mammalian cells.

  1996cited by this paper
• Sequence Analysis and Chromosomal Localization of Human Cap Z

  1995cited by this paper
• The F plasmid CcdB protein induces efficient ATP-dependent DNA cleavage by gyrase.

  1993cited by this paper
• Internal ribosome entry site within hepatitis C virus RNA

  1992cited by this paper
• Immunological detection of phenylalanine hydroxylase protein in Drosophila melanogaster.

  1992cited by this paper
• Use of the human elongation factor 1α promoter as a versatile and efficient expression system

  1990cited by this paper
• Use of the human elongation factor 1 alpha promoter as a versatile and efficient expression system.

  1990cited by this paper
• Isolation and characterization of the human chromosomal gene for polypeptide chain elongation factor-1 alpha.

  1989cited by this paper

• Reconstitution of nuclear envelope subdomain formation on mitotic chromosomes in semi-intact cells

  2024cites this paper
• Current Practice in Bicistronic IRES Reporter Use: A Systematic Review

  2021cites this paper
• Blockade of endoplasmic reticulum stress‐induced cell death by Ureaplasma parvum vacuolating factor

  2021cites this paper
• Molecular farming: strategies, expression systems and bio-safety considerations.

  2018cites this paper
• A molecular toolbox for rapid generation of viral vectors to up- or down-regulate in vivo neuronal gene expression.

  2018cites this paper
• Efficient development of a stable cell pool for antibody production using a single plasmid

  2018cites this paper
• ELYS regulates the localization of LBR by modulating its phosphorylation state

  2016cites this paper
• miR-186 downregulates protein phosphatase PPM1B in bladder cancer and mediates G1-S phase transition

  2016cites this paper
• Combinatorial assembly of clone libraries using site-specific recombination.

  2014cites this paper
• The function activity comparison of eight promoters on glioma cells by gate way system

  2014cites this paper
• Cellular minigene models for the study of allelic expression of the tau gene and its role in progressive supranuclear palsy

  2013cites this paper
• An Internal Ribosome Entry Site (IRES) Mutant Library for Tuning Expression Level of Multiple Genes in Mammalian Cells

  2013cites this paper
• A plasmid toolkit for cloning chimeric cDNAs encoding customized fusion proteins into any Gateway destination expression vector

  2013cites this paper
• Methods for constructing clones for protein expression in mammalian cells.

  2012cites this paper
• Optimizing transient recombinant protein expression in mammalian cells.

  2012cites this paper
• A Molecular Toolbox for Rapid Generation of Viral Vectors to Up- or Down-Regulate Neuronal Gene Expression in vivo

  2011cites this paper
• Modified gateway system for double shRNA expression and Cre/lox based gene expression

  2011cites this paper
• A bicistronic, Ubiquitin-10 promoter-based vector cassette for transient transformation and functional analysis of membrane transport demonstrates the utility of quantitative voltage clamp studies on intact Arabidopsis root epidermis.

  2011cites this paper
• Production of protein complexes via co-expression.

  2011cites this paper
• Molecular Farming: Production of Pharmaceuticals in Transgenic Tobacco

  2011cites this paper
• Multigene Assembly for Construction of Synthetic Operons: Creation and Delivery of an Optimized All-IN-One Expression Construct for Generating Mouse iPS Cells

  2011cites this paper
• hESC engineering by integrase-mediated chromosomal targeting.

  2010cites this paper
• A convenient plasmid-based system containing three reporter genes for real-time and quantitative analysis of messenger RNA silencing.

  2009cites this paper
• A versatile nonviral vector system for tetracycline-dependent one-step conditional induction of transgene expression

  2009cites this paper
• Simultaneous single cell stable expression of 2-4 cDNAs in HeLaS3 using psiC31 integrase system.

  2009cites this paper
• A single EBV-based vector for stable episomal maintenance and expression of GFP in human embryonic stem cells.

  2009cites this paper
• Generation of Platform Human Embryonic Stem Cell Lines That Allow Efficient Targeting at a Predetermined Genomic Location

  2009cites this paper
• In Vitro and In Vivo Expression of Human Erythrocyte Pyruvate Kinase in Erythroid Cells: A Gene Therapy Approach

  2007cites this paper
• Gateway® recombinational cloning: a biological operating system

  2007cites this paper
• A Multicassette Gateway Vector Set for High Throughput and Comparative Analyses in Ciona and Vertebrate Embryos

  2007cites this paper
• Gateway compatible vectors for analysis of gene function in the zebrafish

  2007cites this paper
• Cloning technologies for protein expression and purification.

  2006cites this paper
• Multi-gene gateway clone design for expression of multiple heterologous genes in living cells: modular construction of multiple cDNA expression elements using recombinant cloning.

  2005cites this paper