The yeast Hot1 transcription factor is critical for activating a single target gene, STL1

An active variant of the MAPK Hog1 is used to identify its target genes. The promoter of one target, STL1, possesses a Hog1-responsive element (HoRE) that binds the transcription factor Hot1. HoRE is not found in other promoters, and the STL1 mRNA is the only one abolished in hot1Δ cells. Hot1 may be essential for transcription of one gene.

• Publication year

  2015

• Venue

  Molecular Biology of the Cell

• Publication date

  2015-06-15

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1091/mbc.E14-12-1626PMID 25904326PMCID 4462951
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The p38 Mitogen-activated Protein Kinase Possesses an Intrinsic Autophosphorylation Activity, Generated by a Short Region Composed of the -G Helix and MAPK Insert*

  2014cited by this paper
• From Hypothesis to Mechanism: Uncovering Nuclear Pore Complex Links to Gene Expression

  2014cited by this paper
• Looping back to leap forward: transcription enters a new era.

  2014influential reference
• Transmembrane signaling in Saccharomyces cerevisiae as a model for signaling in metazoans: state of the art after 25 years.

  2014cited by this paper
• Dealing with transcriptional outbursts during S phase to protect genomic integrity.

  2013cited by this paper
• The intertwined roles of transcription and repair proteins.

  2013cited by this paper
• How cells get the message: dynamic assembly and function of mRNA–protein complexes

  2013cited by this paper
• Response to Hyperosmotic Stress

  2012cited by this paper
• Modelling reveals novel roles of two parallel signalling pathways and homeostatic feedbacks in yeast

  2012cited by this paper
• Computational strategies for the genome-wide identification of cis-regulatory elements and transcriptional targets.

  2012cited by this paper
• The Saccharomyces cerevisiae Hot1p regulated gene YHR087W (HGI1) has a role in translation upon high glucose concentration stress

  2012cited by this paper
• Hog1 Controls Global Reallocation of RNA Pol II upon Osmotic Shock in Saccharomyces cerevisiae

  2012influential reference
• Osmostress Induces Autophosphorylation of Hog1 via a C-Terminal Regulatory Region That Is Conserved in p38α

  2012cited by this paper
• Genome-wide transcription factor binding: beyond direct target regulation.

  2011cited by this paper
• Controlling gene expression in response to stress

  2011cited by this paper
• MYC as a regulator of ribosome biogenesis and protein synthesis

  2010cited by this paper
• The yeast MAPK Hog1 is not essential for immediate survival under osmostress

  2009cited by this paper
• p38alpha is active in vitro and in vivo when monophosphorylated at threonine 180.

  2009cited by this paper
• Comparative genomics of gene regulation-conservation and divergence of cis-regulatory information.

  2009cited by this paper
• Structure and function of a transcriptional network activated by the MAPK Hog1

  2008influential reference
• Stress resistance and signal fidelity independent of nuclear MAPK function

  2008cited by this paper
• Isolation of Intrinsically Active (MEK-independent) Variants of the ERK Family of Mitogen-activated Protein (MAP) Kinases*♦

  2008cited by this paper
• Hyperactive Variants of p38α Induce, whereas Hyperactive Variants of p38γ Suppress, Activating Protein 1-mediated Transcription*

  2007cited by this paper
• Hyperactive variants of p38alpha induce, whereas hyperactive variants of p38gamma suppress, activating protein 1-mediated transcription.

  2007cited by this paper
• Intrinsically active variants of all human p38 isoforms

  2007cited by this paper
• Global and gene‐specific analyses show distinct roles for Myod and Myog at a common set of promoters

  2006cited by this paper
• MAP-quest: could we produce constitutively active variants of MAP kinases?

  2006cited by this paper
• The c-Myc target gene network.

  2006cited by this paper
• Identification of human myometrial target genes of the cAMP pathway: the role of cAMP-response element binding (CREB) and modulator (CREMalpha and CREMtau2alpha) proteins.

  2005cited by this paper
• Identification of human myometrial target genes of the c-Jun NH2-terminal kinase (JNK) pathway: the role of activating transcription factor 2 (ATF2) and a novel spliced isoform ATF2-small.

  2005cited by this paper
• Identification of a Novel Class of Target Genes and a Novel Type of Binding Sequence of Heat Shock Transcription Factor in Saccharomyces cerevisiae*

  2005cited by this paper
• Identification of human myometrial target genes of the cAMP pathway: the role of cAMP-response element binding (CREB) and modulator (CREMα and CREMτ2α) proteins

  2005cited by this paper
• Genomewide Identification of Sko1 Target Promoters Reveals a Regulatory Network That Operates in Response to Osmotic Stress in Saccharomyces cerevisiae

  2005cited by this paper
• Structure and evolution of transcriptional regulatory networks.

  2004cited by this paper
• Transcriptional regulatory code of a eukaryotic genome

  2004cited by this paper
• When the Stress of Your Environment Makes You Go HOG Wild

  2004cited by this paper
• Genome-Wide Analysis of the Biology of Stress Responses through Heat Shock Transcription Factor

  2004cited by this paper
• Identification of novel AP-1 target genes in fibroblasts regulated during cutaneous wound healing

  2004cited by this paper
• The MAPK Hog1 recruits Rpd3 histone deacetylase to activate osmoresponsive genes

  2004cited by this paper
• Regulation of the osmoregulatory HOG MAPK cascade in yeast.

  2004cited by this paper
• Combination of Two Activating Mutations in One HOG1 Gene Forms Hyperactive Enzymes That Induce Growth Arrest

  2003cited by this paper
• Phosphorylation of Tyr-176 of the Yeast MAPK Hog1/p38 Is Not Vital for Hog1 Biological Activity* 210

  2003cited by this paper
• Unique and redundant roles for HOG MAPK pathway components as revealed by whole-genome expression analysis.

  2003influential reference
• Osmostress‐induced transcription by Hot1 depends on a Hog1‐mediated recruitment of the RNA Pol II

  2003influential reference
• Yeast go the whole HOG for the hyperosmotic response.

  2002cited by this paper
• Supporting online material for : Transcriptional Regulatory Networks in Saccharomyces cerevisiae

  2002cited by this paper
• Hog1 kinase converts the Sko1-Cyc8-Tup1 repressor complex into an activator that recruits SAGA and SWI/SNF in response to osmotic stress.

  2002cited by this paper
• A Third Osmosensing Branch in Saccharomyces cerevisiae Requires the Msb2 Protein and Functions in Parallel with the Sho1 Branch

  2002cited by this paper
• Osmotic Stress Signaling and Osmoadaptation in Yeasts

  2002cited by this paper
• HSF and Msn2/4p can exclusively or cooperatively activate the yeast HSP104 gene

  2002cited by this paper
• Transcriptional Regulatory Networks in Saccharomyces cerevisiae

  2002cited by this paper
• Isolation of Hyperactive Mutants of the MAPK p38/Hog1 That Are Independent of MAPK Kinase Activation*

  2001cited by this paper
• Transcriptional Profiling Shows that Gcn4p Is a Master Regulator of Gene Expression during Amino Acid Starvation in Yeast

  2001cited by this paper
• Remodeling of yeast genome expression in response to environmental changes.

  2001cited by this paper
• The Transcriptional Response of Saccharomyces cerevisiae to Osmotic Shock

  2000influential reference
• Genomic expression programs in the response of yeast cells to environmental changes.

  2000cited by this paper
• Orchestrated response: a symphony of transcription factors for gene control.

  2000cited by this paper
• The Transcriptional Response of Yeast to Saline Stress*

  2000influential reference
• Osmotic Stress-Induced Gene Expression in Saccharomyces cerevisiae Requires Msn1p and the Novel Nuclear Factor Hot1p

  1999cited by this paper
• The NF-κB Activation Pathway: A Paradigm in Information Transfer from Membrane to Nucleus

  1999cited by this paper
• Msn2p and Msn4p Control a Large Number of Genes Induced at the Diauxic Transition Which Are Repressed by Cyclic AMP inSaccharomyces cerevisiae

  1998cited by this paper
• Control of MAP kinase signaling specificity or how not to go HOG wild.

  1998cited by this paper
• Regulation of transcription by MAP kinase cascades.

  1996cited by this paper
• The HOG pathway controls osmotic regulation of transcription via the stress response element (STRE) of the Saccharomyces cerevisiae CTT1 gene.

  1994cited by this paper
• The yeast and mammalian Ras pathways control transcription of heat shock genes independently of heat shock transcription factor

  1994cited by this paper
• Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression.

  1994cited by this paper
• An osmosensing signal transduction pathway in yeast.

  1993cited by this paper
• Helix-turn-helix, zinc-finger, and leucine-zipper motifs for eukaryotic transcriptional regulatory proteins.

  1989cited by this paper
• Transmembrane signalling in Saccharomyces cerevisiae.

  1989cited by this paper
• Fusion of Escherichia coli lacZ to the cytochrome c gene of Saccharomyces cerevisiae.

  1981influential reference
• Comparative Genomics of Gene Regulation—conservation and Divergence of Cis-regulatory Information This Review Comes from a Themed Issue on Genomes and Evolution Edited Main Text Conflict of Interest

  year unknowncited by this paper

• Hog1 MAP kinase modulates early riboflavin accumulation under low-pH and saline conditions in Debaryomyces hansenii.

  2026cites this paper
• Development of a microbial platform strain for efficient lignocellulosic biomass bioconversion through adaptive evolution and genetic engineering

  2026cites this paper
• A yeast osmotic reporter system as a tool to identify biostimulant for plant drought stress tolerance

  2025cites this paper
• The sugar transporter AsSTL is regulated by the kinase Hog1 and is involved in glycerol transport and the response to osmotic stress in the salt-tolerant ascomycete aspergillus sydowii H-1.

  2025cites this paper
• Direct ionic stress sensing and mitigation by the transcription factor NFAT5

  2025cites this paper
• The Viral K1 Killer Yeast System: Toxicity, Immunity, and Resistance

  2024cites this paper
• Direct ionic stress sensing and mitigation by the transcription factor NFAT5

  2023cites this paper
• Yeast osmoregulation – glycerol still in pole position

  2022cites this paper
• Transcriptome Variations in Verticillium dahliae in Response to Two Different Inorganic Nitrogen Sources

  2021cites this paper
• The high osmolarity glycerol (HOG) pathway in fungi†

  2021influential citation
• MAPK / HOG signaling pathway induced stress‐responsive damage repair is a mechanism for Pichia pastoris to survive from hyperosmotic stress

  2020cites this paper
• Glycerol uptake and synthesis systems contribute to the osmotic tolerance of Kluyveromyces marxianus.

  2020cites this paper
• Hog1-induced transcription of RTC3 and HSP12 is robust and occurs in cells lacking Msn2, Msn4, Hot1 and Sko1

  2020cites this paper
• CgHog1-Mediated CgRds2 Phosphorylation Alters Glycerophospholipid Composition To Coordinate Osmotic Stress in Candida glabrata

  2019cites this paper
• Hyperosmotic Stress Response Memory is Modulated by Gene Positioning in Yeast

  2019cites this paper
• The memory of hyperosmotic stress response in yeast is modulated by gene-positioning

  2019cites this paper
• Functional and expression studies of two novel STL1 genes of the osmotolerant and glycerol utilization yeast Candida glycerinogenes.

  2018cites this paper
• Glycerol stress in Saccharomyces cerevisiae: Cellular responses and evolved adaptations

  2017cites this paper
• The C-terminal region of the Hot1 transcription factor binds GGGACAAA-related sequences in the promoter of its target genes.

  2015cites this paper