PCR-based randomly amplified polymorphic DNA (RAPD) fingerprinting for detection of genetic diversity among Sudanese isolates of Haemophilus somnus

Haemophilus somnus is a pathogen frequently associated with infectious thromboembolic meningoencephalitis in cattle. Five strains of H. somnus were isolated from clinically infected cattle. In the Sudan the organism is associated with a clinical syndrome characterized by nervous manifestations. The strains were found to be morphologically and serologically different as determined by cultural and biochemical characteristics. In the present study, a polymerase chain reaction (PCR)-based randomly amplified polymorphic DNA (RAPD) method was used to amplify H. somnus DNA using arbitrary oligonucleotide primers. The method was able to detect the heterogeneity of amplified DNA from the strains of H. somnus. The scientific data presented in this study suggests that RAPD-PCR could be used as a valuable tool in the study of the the molecular epidemiology of H. somnus isolated from clinical samples of naturally infected cattle. Introduction Haemophilus somnus is a pathogen frequently associated with respiratory and nervous signs. The disease constitutes one of the major veterinary problems in the Sudan (ARADAIB and ABBAS, 1985). The pathogen

• Publication year

  2016

• Venue

  Unknown venue

• Publication date

  2016-05-24

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers

  No identifiers available.

• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

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• No concepts are published for this paper.

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