SCIENCE@home

Vascular damage and delayed cell death in tumours after hyperthermia.

C. Song,M. Kang,J. Rhee,S. Levitt

Published 1980 in British Journal of Cancer

ABSTRACT

CONSIDERABLE INSIGHT into the mechanism of cell death in vitro by hyperthermia has been gained during recent years (Dewey et al., 1977; Hahn, 1974). Besides the direct cytocidal effect of heat, various environmental or physiological factors such as blood flow (Song, 1978; Song et al., 1979) may greatly influence the response of solid tumours in vivo to hyperthermia. In the present study we observed that whereas functional vascular volume remains unchanged during heating in a mammary carcinoma of mice, it decreases drastically after heating. As a consequence, an increasing number of tumour cells die when the tumours are left in situ. The SCK tumour, a mammary carcinoma of A/J mice, was used. This tumour is non-immunogenic or very weakly immunogenic, and grows well in vitro and in vivo. Tumours growing in the flank of female mice were excised, minced and trypsinized. About 5 x 104 cells able to exclude trypan blue were injected s.c. into the leg of female mice. Tumours 7-8 mm in diameter, 8-10 days after the inoculation, were heated and the functional intravascular volume and cell survival assayed. For the heating, we lightly anaesthetized the animals with pentobarbital (0.04 mg/g) and taped them on a plexiglass board. The board was placed over a 43 5°C waterbath and the tumour-bearing legs were immersed in the water through 2-5cm diameter holes in the board. The Accepted 12 November 1979

PUBLICATION RECORD

CITATION MAP

EXTRACTION MAP

CLAIMS

  • No claims are published for this paper.

CONCEPTS

  • No concepts are published for this paper.

REFERENCES

Showing 1-12 of 12 references · Page 1 of 1

CITED BY

Showing 1-93 of 93 citing papers · Page 1 of 1