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Essential oil from Brazilian Red Propolis exhibits anthelmintic activity against larvae of Toxocara cati.

Francine Alves Sinott,Ângela Sena-Lopes,K. Leal,Mara Thaís de Oliveira Silva,Marina Cardoso de Freitas,Micaele Quintana de Moura,Maria Elisabeth Aires Berne,S. Borsuk

Published 2019 in Experimental parasitology

ABSTRACT

Toxocara spp. are responsible for causing toxocariasis, a zoonotic disease of global importance, which is difficult to treat as the available drugs have moderate efficacy in the clinical resolution of the disease. A promising alternative to the existing drugs is Propolis, which is known for having biological and pharmacological properties such as antiparasitic, antioxidant, and antitumor activities. In this study, we report the in vitro anthelmintic activity of essential oil from Brazilian Red Propolis (EOP) against larvae of Toxocara cati. Approximately 100 larvae per well were cultivated in microplates containing RPMI-1640 medium and incubated in the presence of EOP (18.75, 37.5, 75, 150, 300 and 600 μg/mL) to determine the Minimum Inhibitory Concentration (MIC) and IC50 (concentration required to inhibit 50% of the population) values. Then, T. cati larvae treated with the MIC of EOP were inoculated in mice to evaluate their progression in vivo. A concentration of 600 μg/mL of EOP showed 100% larvicidal activity after exposure for 48 h, while 300 μg/mL represented the IC50 and CC50. The anthelmintic activity of EOP was confirmed by the inability of the treated T. cati larvae to infect the mice. Our findings demonstrate the potential of EOP as an anthelmintic.

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