Red clover varieties show nitrogen fixing advantage during the early stages of seedling development

Abstract: Plant and environmental factors affect root nitrogen (N) exudation dynamics in legumes. To better understand the genotypic variability and plant factors affecting root N release nodulation, plant growth, tissue N content, and root N exudation, six (three diploid and three tetraploid) red clover (Trifolium pratense L.) varieties were evaluated under controlled environmental conditions during the first 8 wk of plant growth after rhizobia inoculation. Genotypic differences were found for nodulation, plant dry weight (DW), leaf area, root attributes (root length, surface area, volume, and diameter), shoot and root N concentration, and N content. Genotypic differences were also found for root exudate N content in terms of NO3 --N, NH4 +-N, and dissolved organic N (DON). In general, root exudate inorganic N content was greater in tetraploid varieties than in the diploids throughout the growth period. Root exudate DON content was greater than the inorganic N content. The NO3 --N content in root exudate was positively correlated with root growth attributes and root N concentration, whereas NH4 +-N content was positively correlated with nodule number. Root exudate DON was positively correlated with shoot N concentration and average nodule DW. These results highlight the existence of genotypic differences among red clover varieties for plant morphological factors affecting root N release during the early stages of plant development.

• Publication year

  2017

• Venue

  Canadian Journal of Plant Science

• Publication date

  2017-11-17

• Fields of study

  Agricultural and Food Sciences, Biology, Environmental Science

• Identifiers
  DOI 10.1139/cjps-2017-0071
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Belowground nitrogen transfer from legumes to non-legumes under managed herbaceous cropping systems. A review

  2016cited by this paper
• Nitrogen fixation and transfer of red clover genotypes under legume–grass forage based production systems

  2016cited by this paper
• Erratum to: Belowground nitrogen transfer from legumes to non-legumes under managed herbaceous cropping systems. A review

  2016cited by this paper
• Management of Manure Nitrogen Using Cover Crops

  2015cited by this paper
• Multiple polyploidy events in the early radiation of nodulating and nonnodulating legumes.

  2015cited by this paper
• Red clover (Trifolium pratense L.) draft genome provides a platform for trait improvement

  2015cited by this paper
• Nitrogen losses from the soil/plant system: a review

  2013cited by this paper
• Use of a 15N2 labelling technique to estimate exudation by white clover and transfer to companion ryegrass of symbiotically fixed N

  2013cited by this paper
• Characterizing nitrogen transfer from red clover populations to companion bluegrass under field conditions

  2012cited by this paper
• Genotypic Differences in Root Hair Deformation and Subsequent Nodulation for Red Clover under Different Additions of Starter N Fertilization

  2012cited by this paper
• Nitrogen Rhizodeposition of Legumes

  2011influential reference
• Nitrogen rhizodeposition of legumes. A review

  2011cited by this paper
• Uptake and partitioning of amino acids and peptides.

  2010cited by this paper
• Characterisation of root amino acid exudation in white clover (Trifolium repens L.)

  2010cited by this paper
• Transfer of nitrogen from a tropical legume tree to an associated fodder grass via root exudation and common mycelial networks.

  2009influential reference
• Uptake of organic nitrogen by plants.

  2009cited by this paper
• Root exudates of a legume tree as a nitrogen source for a tropical fodder grass

  2009influential reference
• Is Whole‐Plant Photosynthetic Rate Proportional to Leaf Area? A Test of Scalings and a Logistic Equation by Leaf Demography Census

  2009cited by this paper
• Acquisition of phosphorus and nitrogen in the rhizosphere and plant growth promotion by microorganisms

  2009cited by this paper
• Effects of water and nitrogen availability on nitrogen contribution by the legume, Lupinus argenteus Pursh

  2009cited by this paper
• The influence of water stress on biomass and N accumulation, N partitioning between above and below ground parts and on N rhizodeposition during reproductive growth of pea (Pisum sativum L.)

  2009influential reference
• Use of 15N stable isotope to quantify nitrogen transfer between mycorrhizal plants

  2009cited by this paper
• Dissolved organic nitrogen: an overlooked pathway of nitrogen loss from agricultural systems?

  2009cited by this paper
• Nitrogen rhizodeposition in agricultural crops: Methods, estimates and future prospects

  2008cited by this paper
• Global inputs of biological nitrogen fixation in agricultural systems

  2008cited by this paper
• Tapani red clover

  2008cited by this paper
• A study of 15N transfer between legumes and grasses

  2008influential reference
• Regulation and function of root exudates.

  2008cited by this paper
• Root amino acid exudation: measurement of high efflux rates of glycine and serine from six different plant species

  2007cited by this paper
• Consequences of including adapted white clover in northern European grassland: transfer and deposition of nitrogen

  2007cited by this paper
• Genetic variability in nodulation and root growth affects nitrogen fixation and accumulation in pea.

  2007cited by this paper
• The Nitrogen Transfer Between Plants: An Important but Difficult Flux to Quantify

  2006cited by this paper
• Eukaryotic control on bacterial cell cycle and differentiation in the Rhizobium-legume symbiosis.

  2006influential reference
• Dissolved organic nitrogen uptake by plants—an important N uptake pathway?

  2005cited by this paper
• Genetic regulation of biological nitrogen fixation

  2004cited by this paper
• Legume nodule senescence: roles for redox and hormone signalling in the orchestration of the natural aging process.

  2004cited by this paper
• N transfer from white clover to perennial ryegrass, via exudation of nitrogenous compounds

  2003cited by this paper
• Soybean Nodule Size and Relationship to Nitrogen Fixation Response to Water Deficit

  2001cited by this paper
• Root exudates: a pathway for short-term N transfer from clover and ryegrass

  2001cited by this paper
• Competition for amino acids between wheat roots and rhizosphere microorganisms and the role of amino acids in plant N acquisition

  2001cited by this paper
• Effects of increased atmospheric CO2, temperature, and soil N availability on root exudation of dissolved organic carbon by a N-fixing tree (Robinia pseudoacacia L.)

  2000cited by this paper
• Regulation of biological nitrogen fixation.

  2000cited by this paper
• AC Christie red clover

  1999cited by this paper
• Leaf structure (specific leaf area) modulates photosynthesis–nitrogen relations: evidence from within and across species and functional groups

  1998cited by this paper
• Legume-based cropping systems have reduced carbon and nitrogen losses

  1998cited by this paper
• Rhizodeposition of N by pea and barley and its effect on soil N dynamics

  1996cited by this paper
• Biological nitrogen fixation: An efficient source of nitrogen for sustainable agricultural production?

  1995cited by this paper
• Ontogenetic Changes in Root Nodule Subpopulations of Common Bean (Phaseolus vulgaris L.)III. NODULE FORMATION, GROWTH AND DEGRADATION

  1993cited by this paper
• Ontogenetic Changes in Root Nodule Subpopulations of Common Bean (Phaseolus vulgaris L.) II. PROTEIN CONTENT AND CARBOHYDRATE POOLS

  1993cited by this paper
• Biological nitrogen fixation in mixed legume/grass pastures

  1992cited by this paper
• RED CLOVER.

  1929cited by this paper

• Effects of Soil Steam Sterilization on Red Clover, Its Associated Soil Microorganisms, and Mutualistic Symbionts Under Recurrent Drought

  2026cites this paper
• The Physiological Mechanism of Exogenous Melatonin on Improving Seed Germination and the Seedling Growth of Red Clover (Trifolium pretense L.) under Salt Stress

  2024cites this paper
• Discovering candidate SNPs for resilience breeding of red clover

  2022cites this paper
• Genes Associated with Biological Nitrogen Fixation Efficiency Identified Using RNA Sequencing in Red Clover (Trifolium pratense L.)

  2022cites this paper
• Targeting the Active Rhizosphere Microbiome of Trifolium pratense in Grassland Evidences a Stronger-Than-Expected Belowground Biodiversity-Ecosystem Functioning Link

  2021cites this paper
• Insights Into the Genetic Diversity of Nordic Red Clover (Trifolium pratense) Revealed by SeqSNP-Based Genic Markers

  2021cites this paper
• Changes in Root Architecture and Aboveground Traits of Red Clover Cultivars Driven by Breeding to Improve Persistence

  2020cites this paper
• Allelic Variants for Candidate Nitrogen Fixation Genes Revealed by Sequencing in Red Clover (Trifolium pratense L.)

  2019cites this paper
• Evaluating the Effectiveness of Rhizobium Inoculants and Micronutrients as Technologies for Nepalese Common Bean Smallholder Farmers in the Real-World Context of Highly Variable Hillside Environments and Indigenous Farming Practices

  2019cites this paper
• Soil mineral nitrogen availability predicted by herbage yield and disease resistance in red clover (Trifolium pratense) cropping

  2018cites this paper
• Challenges in Using Precision Agriculture to Optimize Symbiotic Nitrogen Fixation in Legumes: Progress, Limitations, and Future Improvements Needed in Diagnostic Testing

  2018cites this paper
• Visualizing Glutamine Accumulation in Root Systems Involved in the Legume–Rhizobia Symbiosis by Placement on Agar Embedded with Companion Biosensor Cells

  2018cites this paper