Studying the Optimum Conditions of Hygromycin B Production and Detect their Toxicity

تم استخلاص ال hygromycin B بأستخدام خلات الاثيل, فصل المحتوى العضوي عن المائي في راشح المزرعه البكتيرية السائلة, واعطى المحتوى المائي فقط فعالية حيوية بأستخدام تقنية الأنتشار في الحفر على سطح الأغر (Agar well diffusion technique) عند تركيز25ملغم/مل (كمستخلص خام) , اعطى هذا الطور فعالية حيوية ضد مجموعة من الاحياء المجهرية اشتملت على بكتريا واحدة موجبة لغرام (Staphylococcus aureus) وخمس سالبة لغرام Pseudomonas aeruginosa) , Proteus mirabilis,, Escherichia coli, Klebsiella pneumoniae , Salmonella typhi) و خميرة واحدة( (Saccharomyces  cerevisiae.        عند الكشف عن ال hygromycin B aminoglycoside   بأستخدام  كروموتوغرافيا الطبقه الرقيقه لتأكيد وجود المضاد الحيوي, تم الحصول على نفس معدل الجريان (Rf) 0.357 للـ hygromycin B القياسي .        اظهرت نتائج الظروف المثلى للوسط الزرعي البكتيري ان اعلى فعالية حيوية للـ hygromycin B  تم الحصول عليها عند الرقم الهيدروجيني 8  والحضن بحرارة 35 م° لمدة 10 ايام.           تمت دراسة التأثير السمي للـ hygromycin B على كبد الفئران المختبرية وأظهرت تغيرات طفيفه على انسجة كبد الفأر.

• Publication year

  2017

• Venue

  Journal of University of Babylon

• Publication date

  2017-12-26

• Fields of study

  Biology, Chemistry

• Identifiers
  DOI 10.29196/JUB.V26I2.480
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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