Genetic mapping of the rice resistance-breaking gene of the brown planthopper Nilaparvata lugens

Host plant resistance has been widely used for controlling the major rice pest brown planthopper (BPH, Nilaparvata lugens). However, adaptation of the wild BPH population to resistance limits the effective use of resistant rice varieties. Quantitative trait locus (QTL) analysis was conducted to identify resistance-breaking genes against the anti-feeding mechanism mediated by the rice resistance gene Bph1. QTL analysis in iso-female BPH lines with single-nucleotide polymorphism (SNP) markers detected a single region on the 10th linkage group responsible for the virulence. The QTL explained from 57 to 84% of the total phenotypic variation. Bulked segregant analysis with next-generation sequencing in F2 progenies identified five SNPs genetically linked to the virulence. These analyses showed that virulence to Bph1 was controlled by a single recessive gene. In contrast to previous studies, the gene-for-gene relationship between the major resistance gene Bph1 and virulence gene of BPH was confirmed. Identified markers are available for map-based cloning of the major gene controlling BPH virulence to rice resistance.

• Publication year

  2014

• Venue

  Proceedings of the Royal Society B: Biological Sciences

• Publication date

  2014-07-22

• Fields of study

  Agricultural and Food Sciences, Medicine, Biology

• Identifiers
  DOI 10.1098/rspb.2014.0726PMID 24870048PMCID PMC4071553
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• Virulence to Bph1 was controlled by a single recessive gene, and the identified markers provide a basis for map-based cloning of the major gene.

  Confidence 0.91

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• Bulked segregant analysis with next-generation sequencing identified five SNPs genetically linked to virulence.

  Confidence 0.95

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• The detected QTL accounted for 57% to 84% of the phenotypic variation in virulence.

  Confidence 0.94

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• Quantitative trait locus (QTL) analysis with single-nucleotide polymorphism (SNP) markers localized a virulence region to linkage group 10 in brown planthopper (BPH, Nilaparvata lugens).

  Confidence 0.97

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review

• brown planthopper (bph, nilaparvata lugens)

  organism, pest

  A rice-feeding planthopper species used as the insect source for virulence mapping.

  Aliases: BPH, Nilaparvata lugens

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• bulked segregant analysis with next-generation sequencing

  method

  A mapping strategy that compares pooled segregants using sequencing to find linked variants.

  Aliases: bulked segregant analysis, next-generation sequencing-based bulked segregant analysis

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• map-based cloning

  method

  A positional cloning strategy that uses linked markers to isolate a target gene.

  Aliases: positional cloning

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• quantitative trait locus (qtl) analysis

  method

  A linkage-mapping approach used to localize genomic regions associated with a measured trait.

  Aliases: QTL analysis, quantitative trait locus analysis

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• resistance-breaking gene

  gene, genetic factor

  A brown planthopper gene variant associated with overcoming rice resistance.

  Aliases: virulence gene

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• rice resistance gene bph1

  gene, resistance gene

  A rice host resistance gene that mediates anti-feeding defense against brown planthopper.

  Aliases: Bph1

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• single-nucleotide polymorphism (snp) markers

  marker, genetic marker

  DNA markers based on single-base variants used for genetic mapping.

  Aliases: SNP markers

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• single recessive gene

  genetic mechanism

  An inheritance pattern in which a trait is determined by one recessive allele at a locus.

  Aliases: recessive gene

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review
• virulence

  phenotype, trait

  The pest phenotype describing the ability to overcome host resistance.

  Aliases: virulence to rice resistance

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewAK (4715169a40) review

• Trinity : reconstructing a full-length transcriptome without a genome from RNA-Seq data

  2016cited by this paper
• Towards understanding of molecular interactions between rice and the brown planthopper.

  2013cited by this paper
• How do plants achieve immunity? Defence without specialized immune cells

  2012cited by this paper
• A Simple Sequence Repeat- and Single-Nucleotide Polymorphism-Based Genetic Linkage Map of the Brown Planthopper, Nilaparvata lugens

  2012cited by this paper
• Targeting MET in cancer: rationale and progress

  2012cited by this paper
• Combining SNP discovery from next-generation sequencing data with bulked segregant analysis (BSA) to fine-map genes in polyploid wheat

  2012cited by this paper
• Development and use of EST-SSR markers for assessing genetic diversity in the brown planthopper (Nilaparvata lugens Stål)

  2011cited by this paper
• Fast-forward genetics enabled by new sequencing technologies.

  2011cited by this paper
• Brown Planthopper (N. lugens Stal) Feeding Behaviour on Rice Germplasm as an Indicator of Resistance

  2011cited by this paper
• Trinity: reconstructing a full-length transcriptome without a genome from RNA-Seq data

  2011cited by this paper
• A Reproductive Fitness Cost Associated with Hessian Fly (Diptera: Cecidomyiidae) Virulence to Wheat's H Gene-Mediated Resistance

  2011cited by this paper
• Why Does Insect RNA Look Degraded?

  2010cited by this paper
• Baubles, Bangles, and Biotypes: A Critical Review of the use and Abuse of the Biotype Concept

  2010cited by this paper
• IXth International Symposium on Thysanoptera and Tospoviruses, 31 August - 4 September 2009

  2010cited by this paper
• Multi-Phase Defense by the Big-Headed Ant, Pheidole obtusospinosa, Against Raiding Army Ants

  2010cited by this paper
• Current Status of Brown Planthopper (BPH) Resistance and Genetics

  2010cited by this paper
• The Sequence Alignment/Map format and SAMtools

  2009cited by this paper
• Emerging concepts in effector biology of plant-associated organisms.

  2009cited by this paper
• Identification and characterization of Bph14, a gene conferring resistance to brown planthopper in rice

  2009cited by this paper
• JoinMap® 4, Software for the calculation of genetic linkage maps in experimental populations

  2006cited by this paper
• IR varieties and their impact

  2005cited by this paper
• Probing behavior of the brown planthopper, Nilaparvata lugens Stål (Homoptera: Delphacidae) on a non-host barnyard grass, and resistant and susceptible varieties of rice

  2001cited by this paper
• Quantitative genetic analysis of biotypes of the brown planthopper Nilaparvata lugens: heritability of virulence to resistant rice varieties

  1999cited by this paper
• Gene-for-gene coevolution between plants and parasites

  1992cited by this paper
• Population biology of planthoppers

  1990cited by this paper
• Re-examination of Chromosomes of Three Species of Rice Planthoppers(Homoptera : Delphacidae)

  1990cited by this paper
• Electrical penetration graphs from Nilaparvata lugens on resistant and susceptible rice varieties

  1989cited by this paper
• Variety-related feeding patterns in the brown planthopper, Nilaparvata lugens (stål) (Hemiptera: Delphacidae), on its host, the rice plant

  1988cited by this paper
• Electronic Monitoring of Feeding Behavior of Nilaparvata lagens (Homoptera: Delphacidae) on Resistant and Susceptible Rice Cultivars

  1986cited by this paper
• An Evolutionary and Applied Perspective of Insect Biotypes

  1984cited by this paper
• Organization and mapping of a sequence on the Drosophila melanogaster X and Y chromosomes that is transcribed during spermatogenesis.

  1984cited by this paper
• The biotype concept and its application to insect pests of agriculture

  1983cited by this paper
• The influence of host-plant on the development of the adult brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae), and its significance in migration

  1983cited by this paper
• The Rice Brown Planthopper: Feeding Physiology and Host Plant Interactions

  1982cited by this paper
• ADAPTATIONS OF BROWN PLANTHOPPER (NILAPARVATA LUGENS) POPULATIONS TO RICE VARIETIES IN SRI LANKA

  1982cited by this paper
• Parafilm Sachet for Measuring Honeydew Excretion by Nilaparvata lugens on Rice

  1982cited by this paper
• Selection of Biotype Populations 2 and 3 of Nilaparvata lugens by Exposure to Resistant Rice Varieties

  1982cited by this paper
• Hybridization Experiments on Three Biotypes of the Brown Planthopper, Nilaparvata lugens (Homoptera : Delphacidae) at the IRRI, the Philippines

  1981cited by this paper
• THE GENETICS OF THE “BIOTYPES” OF THE RICE BROWN PLANTHOPPER, NILAPARVATA LUGENS

  1981cited by this paper
• Biotypic Variations in the Brown Planthopper, Nilaparvata lugens (Homoptera : Delphacidae)at the IRRI, the Philippines

  1981cited by this paper
• Transport and Partitioning of Nitrogenous Solutes

  1980cited by this paper
• THE “BIOTYPES” OF THE RICE BROWN PLANTHOPPER, NILAPARVATA LUGENS

  1980cited by this paper
• Annual report for 1977

  1978cited by this paper
• Mechanisms of Brown Planthopper Resistance in Mudgo Variety of Rice (Hemiptera : Delphacidae)

  1970cited by this paper
• Resistance to Nephotettix impicticeps and Nilaparvata lugens in Varieties of Rice

  1969cited by this paper
• The Complementary Genic Systems in Flax and Flax Rust

  1956cited by this paper
• BIOINFORMATICS ORIGINAL PAPER

  year unknowncited by this paper

• Plant defense versus pest defense – a case study with Oryza sativa L. and Nilaparvata lugens Stal.

  2025cites this paper
• Insecticide resistance triggers a reduction of virulence to host‐plant defenses in the brown planthopper

  2024cites this paper
• Virulence Adaptation by Rice Planthoppers and Leafhoppers to Resistance Genes and Loci: A Review

  2024cites this paper
• Integrative Omics Strategies for Understanding and Combating Brown Planthopper Virulence in Rice Production: A Review

  2024cites this paper
• Feeding Behavior of Brown Planthopper (Nilaparvata lugens) on Pigmented Rice Monitored by Electrical Penetration Graph (EPG)

  2024cites this paper
• Advances of herbivore-secreted elicitors and effectors in plant-insect interactions

  2023cites this paper
• Expression Analysis Reveals Differentially Expressed Genes in BPH and WBPH Associated with Resistance in Rice RILs Derived from a Cross between RP2068 and TN1

  2023cites this paper
• Development and characterization of near-isogenic lines for brown planthopper resistance genes in the genetic background of japonica rice ‘Sagabiyori’

  2023cites this paper
• Unveiling Nilaparvata lugens Stål Genes Defining Compatible and Incompatible Interactions with Rice through Transcriptome Analysis and Gene Silencing

  2023cites this paper
• Recent Advances in Molecular Mechanism and Breeding Utilization of Brown Planthopper Resistance Genes in Rice: An Integrated Review

  2023cites this paper
• Insecticide resistance reduces the profitability of insect-resistant rice cultivars

  2023cites this paper
• Slowing virulence adaptation in Asian rice planthoppers through migration-based deployment of resistance genes.

  2023cites this paper
• The brown planthopper (Nilaparvata lugens) attack and its genetic diversity on rice in Bali, Indonesia

  2022cites this paper
• Bulked Segregant RNA Sequencing Revealed Difference Between Virulent and Avirulent Brown Planthoppers

  2022cites this paper
• Molecular Approaches for Insect Pest Management in Rice

  2021cites this paper
• SSR MARKERS REVEALED GENETIC DIVERGENCE OF RICE BROWN PLANTHOPPER POPULATIONS MAINTAINED ON TWO SETS OF DIFFERENTIAL HOST VARIETIES

  2021cites this paper
• Genome-wide Analysis of Alternative Gene Splicing Associated with Virulence in the Brown Planthopper Nilaparvata lugens (Hemiptera: Delphacidae)

  2021cites this paper
• Genetic Dissection of the Breakdown of Durable Resistance in Indica Rice Variety PTB33 to Brown Planthoppers Nilaparvata Lugens (Stål)

  2021cites this paper
• Construction of high-density SNP genetic maps and QTL mapping for dwarf-related traits in Litchi chinensis Sonn

  2021cites this paper
• Adaptation by the Brown Planthopper to Resistant Rice: A Test of Female-Derived Virulence and the Role of Yeast-like Symbionts

  2021cites this paper
• SRAP analysis of brown planthopper (Nilaparvata lugens) populations maintained on differential rice host varieties

  2021cites this paper
• Brown planthopper infestations alter sugar metabolism in the rice plant as well as brown planthopper

  2021cites this paper
• Current understanding of the genomic, genetic, and molecular control of insect resistance in rice

  2020cites this paper
• Changes in insecticide resistance and host range performance of planthoppers artificially selected to feed on resistant rice

  2020cites this paper
• BSA-Seq Discovery and Functional Analysis of Candidate Hessian Fly (Mayetiola destructor) Avirulence Genes

  2020cites this paper
• Genetic and molecular understanding of host rice resistance and Nilaparvata lugens adaptation.

  2020cites this paper
• Applications of Biotechnology for Characterization of Plants and Pests as the Key Components of Plant Protection and Production Strategies: A Review

  2020cites this paper
• Combining next-generation sequencing and single-molecule sequencing to explore brown plant hopper responses to contrasting genotypes of japonica rice

  2019cites this paper
• Effects of life history and ecology on virus evolutionary potential.

  2019cites this paper
• Counting Brown Planthoppers in Light-Trap Images Using Morphological Operations

  2019cites this paper
• Phenotypic and transcriptomic responses of two Nilaparvata lugens populations to the Mudgo rice containing Bph1

  2019cites this paper
• Transcriptional regulation of the vitellogenin gene through a fecundity‐related single nucleotide polymorphism within a GATA‐1 binding motif in the brown planthopper, Nilaparvata lugens

  2018cites this paper
• Silencing of miR156 confers enhanced resistance to brown planthopper in rice

  2018cites this paper
• Virulence adaptation in a rice leafhopper: Exposure to ineffective genes compromises pyramided resistance

  2018cites this paper
• Integrating gene deployment and crop management for improved rice resistance to Asian planthoppers

  2018cites this paper
• Kemiripan genetik wereng coklat, Nilaparvata lugens Stål. (Homoptera: Delphacidae) populasi Klaten dan Yogyakarta berdasarkan penanda RAPD-PCR

  2018cites this paper
• Genomics of interaction between the brown planthopper and rice.

  2017cites this paper
• Chromosomal Location of a Recessive Red-Eye Mutant Gene in the Brown Planthopper Nilaparvata lugens (Stål) (Insecta: Hemiptera)

  2017cites this paper
• A Morphological Approach to Counting BPH in Light-Trap Images with Overlapping Individuals

  2017cites this paper
• Loss of Resistance to Nilaparvata lugens May Be Due to the Low-Level Expression of BPH32 in Rice Panicles at the Heading Stage

  2017cites this paper
• Geographic and Research Center Origins of Rice Resistance to Asian Planthoppers and Leafhoppers: Implications for Rice Breeding and Gene Deployment

  2017influential citation
• Benefits and potential trade‐offs associated with yeast‐like symbionts during virulence adaptation in a phloem‐feeding planthopper

  2017cites this paper
• Plant Immunity: Connecting the Dots Between Microbial and Hemipteran Immune Responses

  2016cites this paper
• Management of Rice Planthoppers Through Recent Advanced Research

  2016cites this paper
• An Approach to Detecting Brown Plant Hopper Based on Morphological Operations

  2016cites this paper
• Evolving ideas about genetics underlying insect virulence to plant resistance in rice-brown planthopper interactions.

  2016cites this paper
• Avirulence gene mapping in the Hessian fly (Mayetiola destructor) reveals a protein phosphatase 2C effector gene family.

  2016cites this paper
• Hemipteran and dipteran pests: Effectors and plant host immune regulators.

  2016cites this paper
• Genetic and biochemical mechanisms of rice resistance to planthopper

  2016cites this paper
• Discovery and functional analyses of Hessian fly effector-encoding genes

  2016influential citation
• Virulence of Brown Planthopper and Development of Core Collection of the Pest

  2016cites this paper
• Adaptation of the brown planthopper, Nilaparvata lugens (Sta°l), to resistant rice varieties

  2016cites this paper
• Systematic analysis of rice (Oryza sativa) metabolic responses to herbivory.

  2016cites this paper
• Recent progress on the genetics and molecular breeding of brown planthopper resistance in rice

  2016cites this paper
• Pivoting from Arabidopsis to wheat to understand how agricultural plants integrate responses to biotic stress.

  2015cites this paper
• Phloem-exudate proteome analysis of response to insect brown plant-hopper in rice.

  2015cites this paper
• Insect effectors and gene-for-gene interactions with host plants.

  2015cites this paper
• Genotyping-by-sequencing (GBS) identified SNP tightly linked to QTL for pre-harvest sprouting resistance

  2015cites this paper