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MicroRNA as a Molecular Marker for Predicting the Risk of Intestinal Type Gastric Cancer Development

V. Rubtsov,Y. Fedotova,M. K. Ivanov,S. Titov,E. G. Pomorgailo

Published 2018 in Sovremennye tehnologii v medicine

ABSTRACT

The aim of the study was to assess expression of microRNAs (miRNAs) influencing the key carcinogenesis pathways in the tissues of gastric mucosa in patients with Н. pylori-associated chronic gastritis and gastric cancer of intestinal type (directly in the tumor tissue and mucosa of the stomach with morphological signs of chronic gastritis). Materials and Methods. Objects of investigations were fragments of gastric mucosa of the antral part of the stomach with Н. pyloriassociated chronic gastritis: 10 were bioptates with minimal changes, 9 bioplates with chronic gastritis, and specimens of surgical material taken from the stomachs with gastric cancer of intestinal type: 28 were tumor fragments and 22 the tissue from the sites beyond tumor growth (chronic atrophic gastritis). The number of miRNAs miR-146а, miR-155, miR-21, miR-223, miR-192, miR-200b, miR-221, miR-375 in paraffin sections was determined by a real-time semi-quantitative hairpin RT-PCR assay. Results. The analysis of miR-21 and miR-223 showed the most statistically significant results among the investigated miRNAs. The expression level of oncogenic miR-21 and miR-223 significantly and successively increases in the range of the examined diseases from Н. pylori-associated chronic gastritis to intestinal type gastric adenocarcinoma. Conclusion. MiRNAs miR-21 and miR-223 may be considered as perspective biomarkers for assessing the risk of development, early diagnosis, and prognosis of intestinal type gastric cancer.

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