Reengineering cell-free protein synthesis as a biosensor: Biosensing with transcription, translation, and protein-folding

Abstract Biosensors highjack and reengineer biological systems for highly specific detection of diverse molecules. However, many traditional biosensor devices have slow response times and high operating costs and require specialized training and immobile lab equipment. In an effort to address these limitations, researchers have begun using cell-free protein synthesis (CFPS) systems as biosensors. CFPS-based systems provide the advantages of speed, portability, and robustness at a relatively low cost. Here, we review CFPS-based biosensors according to the three fundamental stages of CFPS: transcription, translation, and protein folding.

• Publication year

  2018

• Venue

  Biochemical engineering journal

• Publication date

  2018-10-01

• Fields of study

  Biology, Chemistry, Engineering

• Identifiers
  DOI 10.1016/J.BEJ.2018.06.014
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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