Secretory production of single-chain antibody (scFv) in Brevibacillus choshinensis using novel fusion partner

No abstract is available for this paper.

• Publication year

  2013

• Venue

  Applied Microbiology and Biotechnology

• Publication date

  2013-01-20

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1007/s00253-013-4695-2PMID 23334514
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Halophilic characterization of starch-binding domain from Kocuria varians α-amylase.

  2012cited by this paper
• Advances in the production and downstream processing of antibodies.

  2011cited by this paper
• Refolding single-chain antibody (scFv) using lauroyl-L-glutamate as a solubilization detergent and arginine as a refolding additive.

  2011cited by this paper
• Therapeutic monoclonal antibodies.

  2011cited by this paper
• Salt-dependent thermo-reversible α-amylase: cloning and characterization of halophilic α-amylase from moderately halophilic bacterium, Kocuria varians

  2011cited by this paper
• Brevibacillus expression system: host-vector system for efficient production of secretory proteins.

  2010cited by this paper
• Recent advances in large-scale production of monoclonal antibodies and related proteins.

  2010cited by this paper
• High solubility supports efficient refolding of thermally unfolded β-lactamase.

  2010cited by this paper
• Alternative antibody formats.

  2010cited by this paper
• Novel soluble expression technologies derived from unique properties of halophilic proteins

  2010cited by this paper
• Halophilic β-lactamase as a new solubility- and folding-enhancing tag protein: production of native human interleukin 1α and human neutrophil α-defensin

  2010cited by this paper
• Strategies and challenges for the next generation of therapeutic antibodies

  2010cited by this paper
• Efficient intraocular penetration of topical anti-TNF-alpha single-chain antibody (ESBA105) to anterior and posterior segment without penetration enhancer.

  2009cited by this paper
• Refolding of scFv mini-antibodies using size-exclusion chromatography via arginine solution layer.

  2009cited by this paper
• Antibody therapeutics, antibody engineering, and the merits of protein stability.

  2008cited by this paper
• Engineering of halophilic enzymes: Two acidic amino acid residues at the carboxy‐terminal region confer halophilic characteristics to Halomonas and Pseudomonas nucleoside diphosphate kinases

  2008cited by this paper
• Stable supply of large amounts of human Fab from the inclusion bodies in E. coli.

  2007cited by this paper
• Antibody constructs in cancer therapy

  2007cited by this paper
• Dimeric structure of nucleoside diphosphate kinase from moderately halophilic bacterium: contrast to the tetrameric Pseudomonas counterpart.

  2007cited by this paper
• Post-genomics of the model haloarchaeon Halobacterium sp. NRC-1

  2006cited by this paper
• Opposing effects of NaCl on reversibility and thermal stability of halophilic beta-lactamase from a moderate halophile, Chromohalobacter sp. 560.

  2006cited by this paper
• Contribution of halophilic nucleoside diphosphate kinase sequence to the heat stability of chimeric molecule.

  2006cited by this paper
• Arginine as an effective additive in gel permeation chromatography.

  2005cited by this paper
• Highly efficient renaturation of β‐lactamase isolated from moderately halophilic bacteria

  2004cited by this paper
• Substantial energetic improvement with minimal structural perturbation in a high affinity mutant antibody.

  2004cited by this paper
• Production technologies for monoclonal antibodies and their fragments.

  2004cited by this paper
• Accuracy in multiangle light scattering measurements for molar mass and radius estimations. Model calculations and experiments.

  2003cited by this paper
• Practical considerations in refolding proteins from inclusion bodies.

  2003cited by this paper
• The role of therapeutic antibodies in drug discovery.

  2003cited by this paper
• Therapeutic antibody production technologies: molecules, applications, expression and purification.

  2001cited by this paper
• Stability engineering of antibody single-chain Fv fragments.

  2001cited by this paper
• High-level production of recombinant chicken interferon-gamma by Brevibacillus choshinensis.

  2001cited by this paper
• Therapeutic antibody production technologies: molecules, applications, expression and purification.

  2001cited by this paper
• High-Level Production of Recombinant Chicken Interferon-γ by Brevibacillus choshinensis

  2001cited by this paper
• Halophilic enzymes: proteins with a grain of salt.

  2000cited by this paper
• Highly efficient recovery of functional single-chain Fv fragments from inclusion bodies overexpressed in Escherichia coli by controlled introduction of oxidizing reagent--application to a human single-chain Fv fragment.

  1998cited by this paper
• Advances in refolding of proteins produced in E. coli.

  1998cited by this paper
• Electrostatic contributions to the stability of halophilic proteins.

  1998cited by this paper
• Biology of Moderately Halophilic Aerobic Bacteria

  1998cited by this paper
• Correct disulfide pairing and efficient refolding of detergent-solubilized single-chain Fv proteins from bacterial inclusion bodies.

  1995cited by this paper
• Effect of the order of antibody variable regions on the expression of the single-chain HyHEL10 Fv fragment in E. coli and the thermodynamic analysis of its antigen-binding properties.

  1994cited by this paper
• Screening and Characterization of Protein-hyperproducing Bacteria without Detectable Exoprotease Activity

  1989cited by this paper
• Measurement of protein using bicinchoninic acid.

  1985cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper

• Developments in the Production of Recombinant Monoclonal Antibodies

  2025cites this paper
• Evaluation of antibody variants using a ribosome display and Brevibacillus choshinensis secretion system.

  2025cites this paper
• Generation of rabbit single-chain variable fragments with different physicochemical and biological properties by complementary determining region-grafting technology.

  2024cites this paper
• Addition of arginine hydrochloride and proline to the culture medium enhances recombinant protein expression in Brevibacillus choshinensis: The case of RBD of SARS-CoV-2 spike protein and its antibody

  2022cites this paper
• Enhanced extracellular α-amylase production in Brevibacillus choshinensis by optimizing extracellular degradation and folding environment

  2021cites this paper
• Brevibacilus thermoruber: thermophilic bacteria isolated from hot spring with the promising potential as a biomolecule producer

  2021cites this paper
• Recombinant blood products and therapeutic enzymes: An update

  2020cites this paper
• Current pivotal strategies leading a difficult target protein to a sample suitable for crystallographic analysis.

  2020cites this paper
• Available strategies for improved expression of recombinant proteins in Brevibacillus expression system: a review

  2020cites this paper
• Extracellular production of recombinant sus scrofa trefoil factor 3 by Brevibacillus choshinensis

  2020cites this paper
• Functional characterization of unique enzymes in Xanthomonas euvesicatoria related to degradation of arabinofurano-oligosaccharides on hydroxyproline-rich glycoproteins

  2018cites this paper
• Efficient production of Trastuzumab Fab antibody fragments in Brevibacillus choshinensis expression system.

  2018cites this paper
• Improved scFv Anti-LOX-1 Binding Activity by Fusion with LOX-1-Binding Peptides

  2017cites this paper
• Mini review: Recombinant production of tailored bio‐pharmaceuticals in different Bacillus strains and future perspectives

  2017cites this paper
• Expression and Characterization of a Single-Chain Variable Fragment against Human LOX-1 in Escherichia coli and Brevibacillus choshinensis.

  2017cites this paper
• Efficient extracellular expression of Bacillus deramificans pullulanase in Brevibacillus choshinensis

  2016cites this paper
• Genomic analysis of Brevibacillus thermoruber 423 reveals its biotechnological and industrial potential

  2015influential citation
• Highly efficient production of VHH antibody fragments in Brevibacillus choshinensis expression system.

  2015cites this paper
• Recombinant antibody production evolves into multiple options aimed at yielding reagents suitable for application-specific needs

  2015influential citation
• Efficient Expression of Antibody Fragments with the Brevibacillus Expression System

  2014cites this paper
• Brevibacillus as a biological tool: a short review

  2014influential citation
• Efficient production of anti-fluorescein and anti-lysozyme as single-chain anti-body fragments (scFv) by Brevibacillus expression system.

  2013cites this paper