Structures of novel sialylated O-linked oligosaccharides isolated from human erythrocyte glycophorins.

The O-linked oligosaccharides attached to human erythrocyte glycophorins were extensively characterized. In addition to the previously described disialylated tetrasaccharide, NeuNAc alpha 2----3Gal beta 1----3 (Neu-NAc alpha 2----6)GalNAcOH and monosialylated trisaccharide, NeuNAc alpha 2----3Gal beta 1----3GalNAcOH, novel trisialylated oligosaccharides were isolated. Methylation analysis, fast atom bombardment-mass spectrometry, and enzymatic degradation were used to elucidate the following novel structures: formula; see text: These results suggest that O-linked oligosaccharides with a disialosyl group, NeuNAc alpha 2----8NeuNAc alpha 2----, may be present in various tissues.

• Publication year

  1987

• Venue

  Journal of Biological Chemistry

• Publication date

  1987-09-05

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/S0021-9258(18)45301-XPMID 3624241
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Separation of anionic oligosaccharides by high-performance liquid chromatography.

  1986cited by this paper
• Polysialoglycoproteins of Salmonidae fish eggs. Complete structure of 200-kDa polysialoglycoprotein from the unfertilized eggs of rainbow trout (Salmo gairdneri).

  1986cited by this paper
• Structural variations of O-linked oligosaccharides present in leukosialin isolated from erythroid, myeloid, and T-lymphoid cell lines.

  1986cited by this paper
• Structures of O-linked oligosaccharides present in the proteoglycans secreted by human mammary epithelial cells.

  1986cited by this paper
• A naturally occurring deaminated neuraminic acid, 3-deoxy-D-glycero-D-galacto-nonulosonic acid (KDN). Its unique occurrence at the nonreducing ends of oligosialyl chains in polysialoglycoprotein of rainbow trout eggs.

  1986cited by this paper
• Structures of O-linked oligosaccharides isolated from normal granulocytes, chronic myelogenous leukemia cells, and acute myelogenous leukemia cells.

  1986cited by this paper
• Structure of the carbohydrate units of human amniotic fluid fibronectin.

  1985cited by this paper
• Embryonal lactosaminoglycan. The structure of branched lactosaminoglycans with novel disialosyl (sialyl alpha 2----9 sialyl) terminals isolated from PA1 human embryonal carcinoma cells.

  1985cited by this paper
• Structure of branched lactosaminoglycan, the carbohydrate moiety of band 3 isolated from adult human erythrocytes.

  1984cited by this paper
• Purification and structures of oligosaccharide chains in swine trachea and Cowper's gland mucin glycoproteins.

  1984cited by this paper
• Structure of fetal lactosaminoglycan. The carbohydrate moiety of Band 3 isolated from human umbilical cord erythrocytes.

  1984cited by this paper
• Novel oligosaccharide chains on polysialoglycoproteins isolated from rainbow trout eggs. A unique carbohydrate sequence with a sialidase-resistant sialyl group, DGa1NAc beta 1 leads to 4(NeuGc2 leads to 3)DGa1NAc.

  1984cited by this paper
• Kinetics of homophilic binding by embryonic and adult forms of the neural cell adhesion molecule.

  1983cited by this paper
• A carbohydrate structural variant of MM glycoprotein (glycophorin A).

  1983cited by this paper
• Biosynthesis of the O-glycosidically linked oligosaccharide chains of fetuin. Indications for an alpha-N-acetylgalactosaminide alpha 2 leads to 6 sialyltransferase with a narrow acceptor specificity in fetal calf liver.

  1983cited by this paper
• Structures of the O-glycosidically linked oligosaccharides of human IgD.

  1983cited by this paper
• Primary structure of the oligosaccharide determinant of blood group Cad specificity.

  1983cited by this paper
• Occurrence of alpha 2-8 linked polysialosyl units in a neural cell adhesion molecule.

  1983cited by this paper
• Preparation and fractionation of glycopeptides.

  1982cited by this paper
• Occurrence of unique polysialosyl carbohydrate units in glycoproteins of developing brain.

  1982cited by this paper
• Structure of O-glycosidically linked sugar units from plasma membranes of an ascites hepatoma, AH 66.

  1982cited by this paper
• Oligosaccharides on proteoglycans from the swarm rat chondrosarcoma.

  1980cited by this paper
• The role of sialic acid in the expression of human MN blood group antigens.

  1979cited by this paper
• Chemical structure of epiglycanin, the major glycoprotein of the TA3-Ha ascites cell. The carbohydrate chains.

  1979cited by this paper
• Methylation analysis of neuraminic acids by gas chromatography-mass spectrometry.

  1977cited by this paper
• Fractionation of the major sialoglycopeptides of the human red blood cell membrane.

  1975cited by this paper
• Structure of the O-glycosidically linked carbohydrate units of fetuin.

  1974cited by this paper
• Alkali-labile oligosaccharides of brain glycoproteins.

  1973cited by this paper
• Isolation and characterization of a glycoprotein from human group O erythrocyte membrane.

  1973cited by this paper
• Structural studies on human erythrocyte glycoproteins. Alkali-labile oligosaccharides.

  1969cited by this paper
• A RAPID PERMETHYLATION OF GLYCOLIPID, AND POLYSACCHARIDE CATALYZED BY METHYLSULFINYL CARBANION IN DIMETHYL SULFOXIDE.

  1964cited by this paper
• THE STRUCTURE AND CHEMISTRY OF COLOMINIC ACID.

  1964cited by this paper
• INACTIVATION OF HUMAN ERYTHROCYTE AGGLUTINOGENS M AND N BY INFLUENZA VIRUSES AND RECEPTOR-DESTROYING ENZYME.

  1958cited by this paper

• Behaviour of a Sialo-Oligosaccharide from Glycophorin in Teleost Red Blood Cell Membranes

  2023cites this paper
• Red Blood Cells Oligosaccharides as Targets for Plasmodium Invasion

  2022cites this paper
• Erythrocyte glycophorins as receptors for Plasmodium merozoites

  2019cites this paper
• A Comprehensive Review of Our Current Understanding of Red Blood Cell (RBC) Glycoproteins

  2017cites this paper
• Chemoenzymatic synthesis of para-nitrophenol (pNP)-tagged α2-8-sialosides and high-throughput substrate specificity studies of α2-8-sialidases.

  2016cites this paper
• Development of novel chemical biology tools to probe malaria parasite physiology and aid in antimalarial drug discovery

  2015cites this paper
• From mass spectrometry-based glycosylation analysis to glycomics and glycoproteomics.

  2014cites this paper
• The sweet spot: defining virus–sialic acid interactions

  2014cites this paper
• Structure of a Sialo-Oligosaccharide from Glycophorin in Carp Red Blood Cell Membranes

  2014cites this paper
• Structural and quantitative changes of carbohydrate chain of erythrocyte membrane glycoproteins in experimental diabetes mellitus after treatment with agmatine

  2013cites this paper
• Adaptation of novel H7N9 influenza A virus to human receptors

  2013cites this paper
• Disialic, oligosialic and polysialic acids: distribution, functions and related disease.

  2013cites this paper
• Advanced mass spectrometry and chemical analyses reveal the presence of terminal disialyl motif on mouse B-cell glycoproteins.

  2013cites this paper
• Universal blood: The life stream all four one and one for all an enzymatic methodology creating a universal blood supply

  2012cites this paper
• O-Linked glycome and proteome of high-molecular-mass proteins in human ovarian cancer ascites: Identification of sulfation, disialic acid and O-linked fucose.

  2012cites this paper
• Glycomic analysis of human mast cells, eosinophils and basophils

  2011cites this paper
• Mammalian sialyltransferase ST3Gal-II: its exchange sialylation catalytic properties allow labeling of sialyl residues in mucin-type sialylated glycoproteins and specific gangliosides.

  2011cites this paper
• Site-specific characterization of threonine, serine, and tyrosine glycosylations of amyloid precursor protein/amyloid β-peptides in human cerebrospinal fluid

  2011cites this paper
• GLUT‐1 glucose transporters in the blood–brain barrier: Differential phosphorylation

  2011cites this paper
• Terminal disialylated multiantennary complex-type N-glycans carried on acutobin define the glycosylation characteristics of the Deinagkistrodon acutus venom.

  2011cites this paper
• A novel series of anti-human glycophorin A (CD235a) antibodies defining five extra- and intracellular epitopes.

  2010cites this paper
• The plasma von Willebrand factor O‐glycome comprises a surprising variety of structures including ABH antigens and disialosyl motifs

  2010cites this paper
• Chemical de‐O‐glycosylation of glycoproteins for application in LC‐based proteomics

  2009cites this paper
• Glycoproteins of erythrocyte membranes and the structure of their carbohydrate determinants

  2009cites this paper
• Chemoenzymatic synthesis of GD3 oligosaccharides and other disialyl glycans containing natural and non-natural sialic acids.

  2009cites this paper
• Single molecular dynamic interactions between glycophorin A and lectin as probed by atomic force microscopy.

  2009cites this paper
• The O-linked glycosylation of secretory/shed MUC1 from an advanced breast cancer patient's serum.

  2008cites this paper
• The Thomsen-Friedenreich disaccharide as antigen for in vivo tumor targeting with multivalent scFvs

  2007cites this paper
• Expression of Aberrant 0-Glycans Attached to Leukosialin in Differentiation- deficient HL-60 Cells1

  2006cites this paper
• Mismatched hemagglutinin and neuraminidase specificities in recent human H3N2 influenza viruses.

  2005cites this paper
• Conditions de traitement de glycoproteines extremement actives et methode efficace de production associee

  2005cites this paper
• Methods in enzymology: O-glycosylation of proteins.

  2005cites this paper
• Structure of the majorO-glycosidic oligosaccharide of monkey erythrocyte glycophorin

  2005cites this paper
• Molecular cloning and expression of a human hST8Sia VI (alpha2,8-sialyltransferase) responsible for the synthesis of the diSia motif on O-glycosylproteins.

  2005cites this paper
• ABH blood group antigens in O-glycans of human glycophorin A.

  2004cites this paper
• Chemoenzymatic synthesis of sialylated oligosaccharides for their evaluation in a polysialyltransferase assay

  2003cites this paper
• Molecular Cloning and Expression of a Sixth Type of α2,8-Sialyltransferase (ST8Sia VI) That SialylatesO-Glycans*

  2002cites this paper
• Antigenic properties of human glycophorins--an update.

  2001cites this paper
• Epitope Diversity of N-Glycans from Bovine Peripheral Myelin Glycoprotein P0 Revealed by Mass Spectrometry and Nano Probe Magic Angle Spinning 1H NMR Spectroscopy*

  2001cites this paper
• Lipids, Membranes, and Cell Coats

  2001cites this paper
• Frequent occurrence of pre-existing alpha 2-->8-linked disialic and oligosialic acids with chain lengths up to 7 Sia residues in mammalian brain glycoproteins. Prevalence revealed by highly sensitive chemical methods and anti-di-, oligo-, and poly-Sia antibodies specific for defined chain lengths.

  2000cites this paper
• Identification of blood group A and B antigens in human glycophorin.

  2000cites this paper
• 'Wave-type' structure of a synthetic hexaglycosylated decapeptide: A part of the extracellular domain of human glycophorin A

  1999cites this paper
• Expression of sialosyl-T and disialosyl-T antigens in erythroid cells.

  1997cites this paper
• Chemistry, biochemistry and biology of sialic acids

  1997cites this paper
• Blood group MN‐dependent difference in degree of galactosylation of O‐glycans of glycophorin A is restricted to the GalNAc residues located on amino acid residues 2–4 of the glycophorin polypeptide chain 1

  1997cites this paper
• Synthesis and characterisation of highly glycosylated glycopeptides with Tn-antigenic structures corresponding to human glycophorin AN.

  1997cites this paper
• Occurrence of terminal alpha 2-->8-linked disialylated poly-N-acetyllactosamine chains with Le(X) and I antigenic glycotopes in tetraantennary arms of an N-linked glycoprotein isolated from rainbow trout ovarian fluid.

  1997cites this paper
• O-linked protein glycosylation structure and function

  1996cites this paper
• Stabilization of the T1 fragment of glycophorin AN through interactions with N- and O-linked glycans

  1996cites this paper
• Specificity Analysis of Murine Monoclonal Antibodies Reactive with Tn, Sialylated Tn, T, and Monosialylated (2 → 6) T Antigens

  1996cites this paper
• Analysis of serine/threonine-linked oligosaccharides derived by alkaline-borohydride treatment of mucin glycoproteins electroblotted onto membranes: comparison of the saccharide profiles of the 390 kDa and 350 kDa forms of epitectin

  1995cites this paper
• Characterization of four monosialo and a novel disialo Asn N-glycosides from the urine of a patient with aspartylglycosaminuria

  1995cites this paper
• Analysis of protein glycosylation by mass spectrometry

  1994cites this paper
• Two different glycosyltransferase defects that result in GalNAc alpha-O-peptide (Tn) expression.

  1994cites this paper
• Biochemical characterization of the O-glycans on recombinant glycophorin A expressed in Chinese hamster ovary cells.

  1994cites this paper
• Blood group antigens on human erythrocytes-distribution, structure and possible functions.

  1994cites this paper
• Aberrant regulation of complement by the erythrocytes of hereditary erythroblastic multinuclearity with a positive acidified serum lysis test (HEMPAS).

  1994cites this paper
• Biochemical characterization of theO-glycans on recombinant glycophorin A expressed in Chinese hamster ovary cells

  1994cites this paper
• The differences in significance of alpha 2,3Gal-linked and alpha 2,6GalNAc-linked sialic acid residues in blood group M- and N-related epitopes recognized by various monoclonal antibodies.

  1994cites this paper
• Structural organization of the mouse glycophorin A gene.

  1994cites this paper
• Vicia villosa B4 lectin is the second anti-Tn lectin shown to react better with blood group N than M antigen

  1994cites this paper
• Physicochemical analyses of oligosaccharide determinants of glycoproteins.

  1994cites this paper
• Characterization of microbial host receptors.

  1994cites this paper
• The HMW1 adhesin of nontypeable Haemophilus influenzae recognizes sialylated glycoprotein receptors on cultured human epithelial cells

  1994cites this paper
• Surface changes to human erythrocytes on infection by Plasmodium falciparum malaria

  1994cites this paper
• Analysis of glycoconjugates by fast atom bombardment mass spectrometry and related ms techniques

  1994cites this paper
• Aberrant regulation of complement by the erythrocytes of hereditary erythroblastic multinuclearity with a positive acidified serum lysis test (HEMPAS)

  1994cites this paper
• Protein glycosylation. Structural and functional aspects.

  1993cites this paper
• Molecular genetics of the glycophorin A gene cluster.

  1993cites this paper
• Carbohydrates and Cytoskeletal Components

  1993cites this paper
• An improved approach to the analysis of the structure of small oligosaccharides of glycoproteins: application to the O-linked oligosaccharides from human glycophorin A.

  1993cites this paper
• Identification of the O-linked glycosylation site of the human transferrin receptor.

  1992cites this paper
• Decrease in erythrocyte glycophorin sialic acid content is associated with increased erythrocyte aggregation in human diabetes.

  1992cites this paper
• O-linked oligosaccharides of glycophorins A and B in erythrocytes of two individuals with the Tn polyagglutinability syndrome.

  1992cites this paper
• Synthesis of the Sialyloligosaccharide Gal-β(1→3)-[Neu5Ac-α(2→6)]-GalNAc, The Epitope of the Tumor-Associated Glycoprotein Tag 72

  1992cites this paper
• The effect of glycosylation trimming enzyme inhibitors on monoclonal antibody recognition of α‐sialoglycoprotein epitopes

  1992cites this paper
• 2 Biochemistry and molecular biology of MNSs blood group antigens

  1991cites this paper
• Immunochemical studies on the differential binding properties of two monoclonal antibodies reacting with Tn red cells

  1991cites this paper
• Expression of aberrant O-glycans attached to leukosialin in differentiation-deficient HL-60 cells.

  1991cites this paper
• Altered O-glycan synthesis in lymphocytes from patients with Wiskott- Aldrich syndrome

  1991cites this paper
• Biochemistry and molecular biology of MNSs blood group antigens.

  1991cites this paper
• Blood Group‐Active Surface Molecules of the Human Red Blood Cell

  1990cites this paper
• Biosynthesis of truncated O-glycans in the T cell line Jurkat. Localization of O-glycan initiation.

  1990cites this paper
• The mechanism of production of multiple mRNAs for human glycophorin A.

  1990cites this paper
• Two-Dimensional NMR Spectral Study (at 500 MHz) of a Glycooctapeptide Derived from Glycophorin AM

  1990cites this paper
• Evidence for a direct role for sialic acid in the attachment of encephalomyocarditis virus to human erythrocytes.

  1990cites this paper
• The Application of High Mass FAB Mass Spectrometry to Molecular Biology

  1989cites this paper
• O-glycosylation of leukosialin in K562 cells. Evidence for initiation and elongation in early Golgi compartments.

  1989cites this paper
• Epitopes on sialoglycoprotein alpha: evidence for heterogeneity in the molecule.

  1989cites this paper
• Normal and abnormal glycosylation probed by fast atom bombardment mass spectrometry.

  1989cites this paper
• Fast atom bombardment-mass spectrometry strategies for analysing glycoprotein glycans.

  1989cites this paper
• Characterization of O-linked saccharides from cell surface glycoproteins.

  1989cites this paper
• Mass spectrometry.

  1988cites this paper
• The structure of glycophorins of animal erythrocytes

  1988cites this paper
• Isolation and characterization of human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Major sialoglycoproteins carrying polylactosaminoglycan.

  1988cites this paper
• Novel fast atom bombardment mass spectrometric procedures for glycoprotein analysis.

  1988cites this paper
• Human T-lymphocyte activation is associated with changes in O-glycan biosynthesis.

  1988cites this paper