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In vitro plant propagation and crop improvement in Lisianthus (Lisianthus Russelianus Hook.)

R. Pop,M. Cantor,E. Buta,I. Csete

Published 2016 in Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture

ABSTRACT

Romania assists at the present time to an increase of production crops for ornamental plants and as a consequence an increased demand of planting material. Thus, improvements of the current multiplication methods are sought after. Lisianthus russelianus Hook. ( Eustoma grandiflorum Grise.) is a relatively new floral crop to the international market, known for beautiful flowers of various colors and for having a long vase life. This study focused on the development of an efficient protocol for rapid regeneration of this species following known basic and applied aspects of lisianthus biotechnology but exploring new potentials. In the course of experiments conducted, for in vitro multiplication there were used nodal segments (1.5 cm) with axillary buds from three F 1 hybrids ‘Echo Lavender’, ‘Flamenco White’, ‘Mirage Pastel Pink’ that were inoculated on MS basal medium supplemented with 0.50 mg 1 -1 TDZ, 1.0 mg 1 -1 BAP and 0.50 mg 1 -1 AIA. The results show that the medium with BAP was most effective for obtaining the highest shoots number compared to medium containing TDZ. For rooting induction, two different concentrations of auxin IBA 0.5 mg 1 -1 and 1.5 mg 1 -1 were used simultaneously on MS basal medium. The highest roots number occurred when using 1.5 mg 1 -1 IBA. Both the number of shoots and rooting regeneration were dependent on the cultivar. The highest shoots number was achieved for ’Mirage Pastel Pink’ hybrid (6.91) on the medium containing 1.0 mg 1 -1 BAP and 0.50 mg 1 -1 IAA.

PUBLICATION RECORD

  • Publication year

    2016

  • Venue

    Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture

  • Publication date

    2016-11-30

  • Fields of study

    Agricultural and Food Sciences, Biology

  • Identifiers
    DOI 10.15835/BUASVMCN-HORT:12367
  • External record

    Open on Semantic Scholar

  • Source metadata

    Semantic Scholar

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