Purification of ribonucleases Sa, Sa2, and Sa3 after expression in Escherichia coli.

No abstract is available for this paper.

• Publication year

  1997

• Venue

  Protein Expression and Purification

• Publication date

  1997-11-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1006/PREP.1997.0776PMID 9367812
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Molecular Cloning: A Laboratory Manual

  2001cited by this paper
• A decade of protein engineering on ribonuclease T1--atomic dissection of the enzyme-substrate interactions.

  1997cited by this paper
• Protein structure : a practical approach

  1997cited by this paper
• Kinetic analysis of the unfolding and refolding of ribonuclease T1 by a stopped-flow double-mixing technique.

  1996cited by this paper
• An evaluation of the use of hydrogen exchange at equilibrium to probe intermediates on the protein folding pathway.

  1996cited by this paper
• Ribonuclease from Streptomyces aureofaciens at atomic resolution.

  1996cited by this paper
• The determinants of pKas in proteins.

  1996cited by this paper
• How to measure and predict the molar absorption coefficient of a protein

  1995cited by this paper
• Complex of ribonuclease from Streptomyces aureofaciens with 2'-GMP at 1.7 A resolution.

  1993cited by this paper
• Complex of ribonuclease Sa with a cyclic nucleotide and a proposed model for the reaction intermediate.

  1993cited by this paper
• Cloning and sequencing of the gene encoding a ribonuclease from Streptomyces aureofaciens CCM3239.

  1992cited by this paper
• Determination and restrained least-squares refinement of the structures of ribonuclease Sa and its complex with 3'-guanylic acid at 1.8 A resolution.

  1991cited by this paper
• Ribonuclease T1: Structure, Function, and Stability

  1991cited by this paper
• Purification of recombinant ribonuclease T1 expressed in Escherichia coli.

  1990cited by this paper
• Contribution of histidine residues to the conformational stability of ribonuclease T1 and mutant Glu-58----Ala.

  1990cited by this paper
• Comparison of active sites of some microbial ribonucleases: structural basis for guanylic specificity.

  1990cited by this paper
• Metabolism and Enzymology of Nucleic Acids: Including Gene Manipulations

  1989cited by this paper
• Expression of the chemically synthesized gene for ribonuclease T1 in Escherichia coli using a secretion cloning vector.

  1988cited by this paper
• Barnase and barstar. Expression of its cloned inhibitor permits expression of a cloned ribonuclease.

  1988cited by this paper
• The X-Ray Analysis of Ribonuclease Sa

  1988cited by this paper
• Conformational stability and activity of ribonuclease T1 with zero, one, and two intact disulfide bonds.

  1988cited by this paper
• Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.

  1987cited by this paper
• Purification of ribonuclease T1.

  1987cited by this paper
• Proteins: Structures and Molecular Properties

  1986cited by this paper
• The structural and sequence homology of a family of microbial ribonucleases

  1983cited by this paper
• Homology between prokaryotic and eukaryotic ribonucleases

  1980cited by this paper
• A new spectrophotometric assay for protein in cell extracts.

  1977cited by this paper
• Exocellular ribonuclease from Streptomyces aureofaciens. II. Properties and specificity.

  1971cited by this paper
• The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts.

  1965cited by this paper
• DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS *

  1964cited by this paper
• The Structure and Function of Ribonuclease T1I. Chromatographic Purification and Properties of Ribonuclease T1

  1961cited by this paper
• Protein measurement with the Folin phenol reagent.

  1951cited by this paper

• Hyperpolarized water via dissolution dynamic nuclear polarization: Applications in biomolecular NMR

  2024cites this paper
• Cracking AlphaFold2: Leveraging the power of artificial intelligence in undergraduate biochemistry curriculums

  2024cites this paper
• 2D NMR spectroscopy of refolding RNase Sa using polarization transfer from hyperpolarized water.

  2021cites this paper
• Characterization of Purified Collagen from Marine Squid Uroteuthis Duvauceli

  2020cites this paper
• An in vitro cytotoxicity of a novel pH-Sensitive lectin loaded-cockle shell-derived calcium carbonate nanoparticles against MCF-7 breast tumour cell

  2020cites this paper
• Effect of Salts and Organic Osmolytes is Due to Conservative Forces

  2020cites this paper
• Observation of Fast 2D NMR Spectra During Protein Folding Using Polarization Transfer from Hyperpolarized Water.

  2019cites this paper
• Microbial ribonucleases (RNases): production and application potential

  2015cites this paper
• Microbial ribonucleases (RNases): production and application potential

  2015influential citation
• Cytotoxicity of RNase Sa to the acute myeloid leukemia Kasumi-1 cells depends on the net charge

  2014cites this paper
• Contribution of hydrogen bonds to protein stability

  2014cites this paper
• Gemini surfactants affect the structure, stability, and activity of ribonuclease Sa.

  2014cites this paper
• The Effects of Buried Ionizable Amino Acids on the Stability of Ribonuclease Sa

  2014cites this paper
• Local and long-range electrostatic interactions in denatured Ribonuclease Sa: comparing the denatured protein with model peptides

  2013cites this paper
• Increased alkalotolerant and thermostable ribonuclease (RNase) production from alkaliphilic Streptomyces sp. M49-1 by optimizing the growth conditions using response surface methodology

  2013cites this paper
• Toward a molecular understanding of protein solubility: increased negative surface charge correlates with increased solubility.

  2012cites this paper
• Contribution of hydrophobic interactions to protein stability.

  2011cites this paper
• Towards a molecular understanding of protein solubility

  2011cites this paper
• Urea denatured state ensembles contain extensive secondary structure that is increased in hydrophobic proteins

  2010cites this paper
• Flexibility observed in high resolution structures of Streptomyces aureofaciens ribonucleases determined by diffraction methods

  2010cites this paper
• Increasing protein stability: Importance of ΔCp and the denatured state

  2010cites this paper
• Synthesis of aligned hematite nanoparticles on chitosan-alginate films.

  2009cites this paper
• Increasing protein stability by improving beta‐turns

  2009cites this paper
• UNDERSTANDING FORCES THAT CONTRIBUTE TO PROTEIN STABILITY: APPLICATION FOR INCREASING PROTEIN STABILITY

  2009influential citation
• Carbodiimide EDC induces cross-links that stabilize RNase A C-dimer against dissociation: EDC adducts can affect protein net charge, conformation, and activity.

  2009cites this paper
• Structure of RNase Sa2 complexes with mononucleotides – new aspects of catalytic reaction and substrate recognition

  2009cites this paper
• Tryptophan fluorescence reveals the presence of long-range interactions in the denatured state of ribonuclease Sa.

  2008cites this paper
• Factors determining the pKa values of the ionizable groups in proteins: their intrinsic pKas and the effects of hydrogen bonding on buried carboxyl groups

  2007cites this paper
• Amino acid contribution to protein solubility: Asp, Glu, and Ser contribute more favorably than the other hydrophilic amino acids in RNase Sa.

  2007cites this paper
• Increasing protein conformational stability by optimizing beta-turn sequence.

  2007cites this paper
• Increasing protein conformational stability by optimizing β-turn sequence

  2007cites this paper
• Low molecular weight chitosan is an efficient inhibitor of ribonucleases.

  2007cites this paper
• Hydrogen bonding markedly reduces the pK of buried carboxyl groups in proteins.

  2006cites this paper
• Molecular basis for nucleotide-binding specificity: role of the exocyclic amino group "N2" in recognition by a guanylyl-ribonuclease.

  2006cites this paper
• Production of Recombinant RNase Ba and Its Application in Downstream Processing of Plasmid DNA for Pharmaceutical Use

  2006cites this paper
• The salt-dependence of a protein-ligand interaction: ion-protein binding energetics.

  2005cites this paper
• Ribonuclease Sa conformational stability studied by NMR-monitored hydrogen exchange.

  2005cites this paper
• Purification and partial characterization of an extracellular ribonuclease from a mutant of Aspergillus niger

  2005cites this paper
• Charge–charge interactions in the denatured state influence the folding kinetics of ribonuclease Sa

  2005cites this paper
• Asp79 makes a large, unfavorable contribution to the stability of RNase Sa.

  2005cites this paper
• Computational and experimental investigations of forces in protein folding

  2005cites this paper
• The folding kinetics of ribonuclease Sa and a charge-reversal variant

  2005cites this paper
• Crystal structure reveals two alternative conformations in the active site of ribonuclease Sa2.

  2004cites this paper
• Zinc(II)-mediated inhibition of ribonuclease Sa by an N-hydroxyurea nucleotide and its basis.

  2004cites this paper
• Energetic consequences of structural features and dynamics changes upon nucleotide binding to ribonuclease SA: molecular basis for nucleotide binding specificity

  2004cites this paper
• Mutational and structural-based analyses of the osmolyte effect on protein stability.

  2004cites this paper
• Energetics of ion-protein interactions

  2004cites this paper
• Probing the denatured state ensemble with fluorescence

  2004influential citation
• THE FOLDING KINETICS OF RIBONUCLEASE Sa AND A CHARGE-REVERSAL VARIANT

  2004influential citation
• Contribution of single tryptophan residues to the fluorescence and stability of ribonuclease Sa.

  2004cites this paper
• Cytotoxicity of RNases is increased by cationization and counteracted by K(Ca) channels.

  2004cites this paper
• The role of protein stability, solubility, and net charge in amyloid fibril formation

  2003cites this paper
• pK values of histidine residues in ribonuclease Sa: effect of salt and net charge.

  2003cites this paper
• The Contribution of Polar Group Burial to Protein Stability Is Strongly Context-dependent*

  2003cites this paper
• Contribution of active site residues to the activity and thermal stability of ribonuclease Sa

  2003cites this paper
• Changing the net charge from negative to positive makes ribonuclease Sa cytotoxic

  2002cites this paper
• Charge-charge interactions are the primary determinants of the pK values of the ionizable groups in Ribonuclease T1.

  2002cites this paper
• Atomic resolution data reveal flexibility in the structure of RNase Sa.

  2002cites this paper
• X-ray Structure of Two Crystalline Forms of aStreptomycete Ribonuclease with Cytotoxic Activity*

  2002cites this paper
• Tyrosine hydrogen bonds make a large contribution to protein stability.

  2001cites this paper
• The effect of net charge on the solubility, activity, and stability of ribonuclease Sa

  2001influential citation
• Solution structure and dynamics of ribonuclease Sa

  2001cites this paper
• Probing functional perfection in substructures of ribonuclease T1: double combinatorial random mutagenesis involving Asn43, Asn44, and Glu46 in the guanine binding loop.

  2001cites this paper
• Purification, crystallization and preliminary X-ray analysis of two crystal forms of ribonuclease Sa3.

  2001cites this paper
• Letter to the Editor: Sequential assignment and solution secondary structure of doubly labelled ribonuclease Sa

  1999cites this paper
• Increasing protein stability by altering long‐range coulombic interactions

  1999cites this paper
• Conformational stability and thermodynamics of folding of ribonucleases Sa, Sa2 and Sa3.

  1998influential citation
• Recognition of RNase Sa by the inhibitor barstar: structure of the complex at 1.7 A resolution.

  1998cites this paper
• Contribution of a conserved asparagine to the conformational stability of ribonucleases Sa, Ba, and T1.

  1998cites this paper