A combination of xylooligosaccharides and a polyphenol blend affect microbial composition and activity in the distal colon exerting immunomodulating properties on human cells

Abstract A validated in vitro gut model (i.e. SHIME®), coupled with co-cultures of epithelial cells and macrophages, was used to investigate the prebiotic potential and immune modulation of long-term administration of a xylooligosaccharides/polyphenols blend. The blend was well fermented along the entire colon leading to increased production of butyrate (+7 mmol/L) in the ascending colon; acetate, propionate and butyrate (approx. +4 mmol/L each) in the transverse colon; propionate (+4.1 mmol/L) in the descending colon. The treatment led to a decrease in ammonium and increased lactate levels in the ascending colon, which correlated with an increase in lactobacilli and bifidobacteria (+2.5 Log/mL). Additionally, the propionate-producing Akkermansia muciniphila increased in the lumen of the proximal colon (up to 7.15 log/mL). Finally, it was observed a protective effect on Caco-2 barrier function in the distal colon and pronounced immune activating properties. The generated data support a possible role in protecting against obesity-related adverse effects.

• Publication year

  2018

• Venue

  Journal of Functional Foods

• Publication date

  2018-08-01

• Fields of study

  Biology, Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1016/J.JFF.2018.05.053
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• In vitro colonisation of the distal colon by Akkermansia muciniphila is largely mucin and pH dependent.

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  2016cited by this paper
• Prebiotic Effects of Xylooligosaccharides on the Improvement of Microbiota Balance in Human Subjects

  2016cited by this paper
• New Dietary Supplements for Obesity: What We Currently Know

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• Polyphenol-Rich Propolis Extracts Strengthen Intestinal Barrier Function by Activating AMPK and ERK Signaling

  2016cited by this paper
• Dietary Polyphenols Promote Growth of the Gut Bacterium Akkermansia muciniphila and Attenuate High-Fat Diet–Induced Metabolic Syndrome

  2015cited by this paper
• Bioavailability of Dietary Polyphenols and Gut Microbiota Metabolism: Antimicrobial Properties

  2015cited by this paper
• Xylooligosaccharides as prebiotics from agricultural by-products: Production and applications

  2015cited by this paper
• Addition of acacia gum to a FOS/inulin blend improves its fermentation profile in the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®)

  2015cited by this paper
• The Simulator of the Human Intestinal Microbial Ecosystem (SHIME

  2015cited by this paper
• Anti-inflammatory effect of chlorogenic acid on the IL-8 production in Caco-2 cells and the dextran sulphate sodium-induced colitis symptoms in C57BL/6 mice.

  2015cited by this paper
• In vitro colonic metabolism of coffee and chlorogenic acid results in selective changes in human faecal microbiota growth

  2015cited by this paper
• A Survey of Modulation of Gut Microbiota by Dietary Polyphenols

  2015cited by this paper
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  2015cited by this paper
• Comparative genomics analysis of Lactobacillus species associated with weight gain or weight protection

  2014cited by this paper
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  2014cited by this paper
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  2014cited by this paper
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  2014influential reference
• Cross-talk between Akkermansia muciniphila and intestinal epithelium controls diet-induced obesity

  2013cited by this paper
• Modulation of Immune Function by Polyphenols: Possible Contribution of Epigenetic Factors

  2013cited by this paper
• Analysis of the microbial gene landscape and transcriptome for aromatic pollutants and alkane degradation using a novel internally calibrated microarray system.

  2013cited by this paper
• Polyphenols protect the epithelial barrier function of Caco-2 cells exposed to indomethacin through the modulation of occludin and zonula occludens-1 expression.

  2013cited by this paper
• Effect of a straw-derived xylooligosaccharide on broiler growth performance, endocrine metabolism, and immune response.

  2013cited by this paper
• Fecal microbial metabolism of polyphenols and its effects on human gut microbiota.

  2013cited by this paper
• Comparative meta-analysis of the effect of Lactobacillus species on weight gain in humans and animals.

  2012cited by this paper
• Gut microbial metabolism of polyphenols from black tea and red wine/grape juice is source-specific and colon-region dependent.

  2012cited by this paper
• Xylo-oligosaccharide (XOS) in combination with inulin modulates both the intestinal environment and immune status in healthy subjects, while XOS alone only shows prebiotic properties.

  2012cited by this paper
• Butyrate-producing Clostridium cluster XIVa species specifically colonize mucins in an in vitro gut model

  2012cited by this paper
• Xylooligosaccharides (XOS) as an Emerging Prebiotic: Microbial Synthesis, Utilization, Structural Characterization, Bioactive Properties, and Applications

  2011cited by this paper
• The host selects mucosal and luminal associations of coevolved gut microorganisms: a novel concept.

  2011cited by this paper
• Responses of Gut Microbiota and Glucose and Lipid Metabolism to Prebiotics in Genetic Obese and Diet-Induced Leptin-Resistant Mice

  2011cited by this paper
• In vitro batch fecal fermentation comparison of gas and short-chain fatty acid production using "slowly fermentable" dietary fibers.

  2011cited by this paper
• Incorporating a mucosal environment in a dynamic gut model results in a more representative colonization by lactobacilli

  2011cited by this paper
• Prebiotic and Other Health-Related Effects of Cereal-Derived Arabinoxylans, Arabinoxylan-Oligosaccharides, and Xylooligosaccharides

  2011cited by this paper
• Mucolytic Bacteria With Increased Prevalence in IBD Mucosa Augment In Vitro Utilization of Mucin by Other Bacteria

  2010cited by this paper
• Human faecal microbiota display variable patterns of glycerol metabolism.

  2010cited by this paper
• In vitro bioconversion of polyphenols from black tea and red wine/grape juice by human intestinal microbiota displays strong interindividual variability.

  2010cited by this paper
• Xylo-oligosaccharides enhance the growth of bifidobacteria and Bifidobacterium lactis in a simulated colon model.

  2010cited by this paper
• The intestinal environment in health and disease - recent insights on the potential of intestinal bacteria to influence human health.

  2009cited by this paper
• Intestinal barrier function: molecular regulation and disease pathogenesis.

  2009cited by this paper
• Control of Pathogenic and Spoilage Microorganisms in Fresh-cut Fruits and Fruit Juices by Traditional and Alternative Natural Antimicrobials.

  2009cited by this paper
• Comparative assessment of human and farm animal faecal microbiota using real-time quantitative PCR.

  2009cited by this paper
• The Effect of Diet on the Human Gut Microbiome: A Metagenomic Analysis in Humanized Gnotobiotic Mice

  2009cited by this paper
• Development of a real‐time PCR method for Firmicutes and Bacteroidetes in faeces and its application to quantify intestinal population of obese and lean pigs

  2008cited by this paper
• Review article: the role of butyrate on colonic function

  2007cited by this paper
• Intestinal Integrity and Akkermansia muciniphila, a Mucin-Degrading Member of the Intestinal Microbiota Present in Infants, Adults, and the Elderly

  2007cited by this paper
• Effects of Xylooligosaccharide Intake on Fecal Bifidobacteria, Lactic acid and Lipid Metabolism in Korean Young Women

  2007cited by this paper
• Colonic Health: Fermentation and Short Chain Fatty Acids

  2006cited by this paper
• Non-digestible Oligosaccharides with Prebiotic Properties

  2006cited by this paper
• Phenolic compounds in coffee

  2006cited by this paper
• Diversity of the Human Intestinal Microbial Flora

  2005cited by this paper
• A place for dietary fibre in the management of the metabolic syndrome

  2005cited by this paper
• Molecular Targets of Dietary Polyphenols with Anti-inflammatory Properties

  2005cited by this paper
• Lactate is mainly fermented to butyrate by human intestinal microfloras but inter‐individual variation is evident

  2005cited by this paper
• Prebiotic effects of chicory inulin in the simulator of the human intestinal microbial ecosystem.

  2004cited by this paper
• Development of an extensive set of 16S rDNA‐targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real‐time PCR

  2004cited by this paper
• PCR-DGGE-based quantification of stability of the microbial community in a simulator of the human intestinal microbial ecosystem.

  2004cited by this paper
• Polyphenols: food sources and bioavailability.

  2004cited by this paper
• Chlorogenic acid bioavailability largely depends on its metabolism by the gut microflora in rats.

  2003cited by this paper
• Molecular Diversity of Lactobacillus spp. and Other Lactic Acid Bacteria in the Human Intestine as Determined by Specific Amplification of 16S Ribosomal DNA

  2002cited by this paper
• Isolation and characterization of human colonic bacteria able to hydrolyse chlorogenic acid

  2001cited by this paper
• A comparative in vitro evaluation of the fermentation properties of prebiotic oligosaccharides

  2001cited by this paper
• Short-chain fructo-oligosaccharide administration dose-dependently increases fecal bifidobacteria in healthy humans.

  1999cited by this paper
• A computer-controlled system to simulate conditions of the large intestine with peristaltic mixing, water absorption and absorption of fermentation products

  1999cited by this paper
• Distribution of bacterioplankton in meromictic Lake Saelenvannet, as determined by denaturing gradient gel electrophoresis of PCR-amplified gene fragments coding for 16S rRNA

  1997cited by this paper
• Health benefits of non-digestible oligosaccharides.

  1997cited by this paper
• Selected indigestible oligosaccharides affect large bowel mass, cecal and fecal short-chain fatty acids, pH and microflora in rats.

  1997cited by this paper
• Effect of propionate on fatty acid and cholesterol synthesis and on acetate metabolism in isolated rat hepatocytes

  1995cited by this paper
• Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics.

  1995cited by this paper
• Development of a 5-step multi-chamber reactor as a simulation of the human intestinal microbial ecosystem

  1993cited by this paper
• Comparison of fermentation reactions in different regions of the human colon.

  1992cited by this paper
• Effects of Administration of Galactooligosaccharides on the Human Faecal Microflora, Stool Weight and Abdominal Sensation

  1990cited by this paper
• Studies on mixed populations of human intestinal bacteria grown in single-stage and multistage continuous culture systems

  1989cited by this paper
• Fermentation in the human large intestine and the available substrates.

  1987cited by this paper

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  2023influential citation
• Tannic acid delaying metabolism of resistant starch by gut microbiota during in vitro human fecal fermentation.

  2023cites this paper
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  2022cites this paper
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