A study of the urinary excretion of vitamin B6 by a colorimetric method.

J. V. Scum,K. Unna,W. Antopol

Published 1940 in Journal of Biological Chemistry

ABSTRACT

Chemical methods for the determination of biological substances, in contrast with animal assays, offer a considerable saving in time, and the analytical data are usually more precise and reproducible. For these reasons, a study of the vitamin Bgindophenol color reaction has been in progress in these laboratories for some time (1). In the course of this work a procedure has been devised whereby solutions containing a minimum of 2 micrograms of the vitamin per cc. can be analyzed with precision (2). The general applicability of this method, the nature of interfering substances, and the specificity of the test are under investigation. Details of this work will be presented elsewhere.

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