Effects of PEG-induced drought stress on regulation of indole alkaloid biosynthesis in Catharanthus roseus

ABSTRACT Catharanthus roseus (C. roseus) plants were used to investigate the terpenoid indole alkaloids (TIAs) accumulation under the condition of PEG-induced drought stress. Multivariate analysis showed that 35% PEG6000 could induce more obvious and stable accumulation on proline (PRO) content and the relative water content (RWC). The results indicated that there were gradual increase and then decrease (p < .05) in the contents of vindoline (VIN) and catharanthine (CAT) under 35% PEG6000 stress, but the content of vinblastine (VBL) increased gradually. In addition, the expression levels of tryptophan decarboxylase (TDC), strictosidine synthase (STR) and deacetylvindoline-4-O-acetyltransferase (DAT) were upregulated in plants under 35% PEG6000 stress. Further correlation analysis indicated that CAT accumulation was significantly correlated with TDC gene expression, and VBL accumulation was significantly correlated with peroxidase (p < .05). Our results suggest that the cultivation of C. roseus in drought stress would serve as effective treatment for accumulating TIAs.

• Publication year

  2017

• Venue

  Journal of Plant Interactions

• Publication date

  2017-01-01

• Fields of study

  Biology, Environmental Science

• Identifiers
  DOI 10.1080/17429145.2017.1293852
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Catharanthus roseus alkaloids: application of biotechnology for improving yield

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  2012cited by this paper
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  2011influential reference
• Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants.

  2010influential reference
• [Effects of NaCl on the growth and alkaloid content of Catharanthus roseus seedlings].

  2008cited by this paper
• A simplified procedure for indole alkaloid extraction from Catharanthus roseus combined with a semi-synthetic production process for vinblastine.

  2007cited by this paper
• Expression of deacetylvindoline-4-O-acetyltransferase in Catharanthus roseus hairy roots.

  2007cited by this paper
• UV-B-induced signaling events leading to enhanced-production of catharanthine in Catharanthus roseus cell suspension cultures

  2007cited by this paper
• Gene-to-metabolite networks for terpenoid indole alkaloid biosynthesis in Catharanthus roseus cells.

  2006cited by this paper
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  2003cited by this paper
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  2002cited by this paper
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  1998cited by this paper
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  1998cited by this paper
• A critical evaluation of traits for improving crop yields in water-limited environments.

  1990cited by this paper
• Recent Advances in Indole Alkaloid Production by Catharanthus roseus (Periwinkle)

  1990cited by this paper

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