Immobilization of Amano lipase from Pseudomonas fluorescens on silk fibroin spheres: an alternative protocol for the enantioselective synthesis of halohydrins

The search for a new, efficient, cheaper and sustainable matrix for lipase immobilization is a growing area in biotechnology. Amano lipase from Pseudomonas fluorescens was immobilized on silk fibroin spheres and used in the enzymatic kinetic resolution of halohydrins, to obtain optically active epoxides (up to 99% ee), important precursors in the synthesis of derivative antifungal azoles. This paper reinforces the versatility of silk fibroin as a support for heterogeneous catalysts.

• Publication year

  2017

• Venue

  RSC Advances

• Publication date

  2017-02-21

• Fields of study

  Materials Science, Chemistry, Environmental Science

• Identifiers
  DOI 10.1039/C7RA00083A
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Ethylic Biodiesel Production Using Lipase Immobilized in Silk Fibroin-Alginate Spheres by Encapsulation

  2016cited by this paper
• Highly active, stable and self-antimicrobial enzyme catalysts prepared by biomimetic mineralization of copper hydroxysulfate.

  2016cited by this paper
• Metal–organic frameworks and inorganic nanoflowers: a type of emerging inorganic crystal nanocarrier for enzyme immobilization

  2015cited by this paper
• Polydopamine tethered enzyme/metal-organic framework composites with high stability and reusability.

  2015cited by this paper
• Facile synthesis of multiple enzyme-containing metal-organic frameworks in a biomolecule-friendly environment.

  2015cited by this paper
• Biocatalysis and biotransformation in Brazil: An overview.

  2015cited by this paper
• Fast Microwave-Assisted Resolution of (±)-Cyanohydrins Promoted by Lipase from Candida antarctica

  2015cited by this paper
• IMMOBILIZATION OF LIPASE ON WOOLLEN FABRICS: ENHANCED EFFECTIVENESS IN STAIN REMOVAL

  2014cited by this paper
• Highly enantioselective acylation of chlorohydrins using Amano AK lipase from P. fluorescens immobilized on silk fibroin–alginate spheres

  2014cited by this paper
• One-pot synthesis of protein-embedded metal-organic frameworks with enhanced biological activities.

  2014cited by this paper
• Enzymatic Esterification of Oleic Acid with Aliphatic Alcohols for the Biodiesel Production by Candida antarctica Lipase

  2013cited by this paper
• Protein-inorganic hybrid nanoflowers.

  2012cited by this paper
• Effect of kapok fiber treated with various solvents on oil absorbency

  2012cited by this paper
• Asymmetric chemoenzymatic synthesis of miconazole and econazole enantiomers. The importance of chirality in their biological evaluation.

  2011cited by this paper
• Catalytic ethanolysis of soybean oil with immobilized lipase from Candida antarctica and 1H NMR and GC quantification of the ethyl esters (biodiesel) produced

  2011cited by this paper
• Immobilization of Rhizopus oryzae lipase on silica aerogels by adsorption: Comparison with the free enzyme

  2011cited by this paper
• Highly regio- and diastereoselective halohydroxylation of olefins: a facile synthesis of vicinal halohydrins

  2011cited by this paper
• Studies of Fe3O4-chitosan nanoparticles prepared by co-precipitation under the magnetic field for lipase immobilization

  2011cited by this paper
• Preparation and Characterization of Insoluble Silk Fibroin/Chitosan Blend Films

  2010cited by this paper
• Heme Oxygenase Inhibition by 1‐Aryl‐2‐(1H‐imidazol‐1‐yl/1H‐1,2,4‐triazol‐1‐yl)ethanones and Their Derivatives

  2010cited by this paper
• Electrospinning from room temperature ionic liquids for biopolymer fiber formation

  2010cited by this paper
• Tellurium in organic synthesis: the enantioselective synthesis of the pheromone blend components of Mayetiola destructor, Drosophila mulleri and Contarinia pisi

  2009cited by this paper
• Lipase bound cellulose nanofibrous membrane via Cibacron Blue F3GA affinity ligand

  2009cited by this paper
• Towards chiral diamines as chiral catalytic precursors for the borane-mediated enantioselective reduction of prochiral ketones

  2009cited by this paper
• Azole antimicrobial pharmacophore-based tetrazoles: synthesis and biological evaluation as potential antimicrobial and anticonvulsant agents.

  2009cited by this paper
• Synthesis of new 1-[2-Azido-2-(2,4-dichlorophenyl)ethyl]-1H/-imidazoles and in vitro evaluation of their antifungal activity

  2008cited by this paper
• Enzymatic resolution of α-tetralols by CALB-catalyzed acetylation

  2007cited by this paper
• Solution behavior of synthetic silk peptides and modified recombinant silk proteins

  2006cited by this paper
• Comparison of hydrolytic activities in aqueous and organic media for lipases immobilized on poly（acrylonitrile-co-maleic acid） ultrafiltration hollow fiber membrane

  2005influential reference
• Mapping domain structures in silks from insects and spiders related to protein assembly.

  2004cited by this paper
• Mechanical Properties of Electrospun Silk Fibers

  2004cited by this paper
• Applications of natural silk protein sericin in biomaterials.

  2002cited by this paper
• Chemoenzymatic dynamic kinetic resolution of beta-halo alcohols. An efficient route to chiral epoxides.

  2002cited by this paper
• Industrial biocatalysis today and tomorrow

  2001cited by this paper
• Natural silk fibroin as a support for enzyme immobilization

  1998cited by this paper
• Concanavalin A: a useful ligand for glycoenzyme immobilization--a review.

  1991cited by this paper

• Silk Fibroin‐Stabilized Lapachol Microemulsion Enhances Antiglioma Activity In Vitro

  2026influential citation
• Effect of Silk Fibroin as a Sustainable Solvent on the Extraction of Bixin from Annatto Seeds (Bixa orellana L.)

  2025cites this paper
• Synthesis of Cellulose Hexanoate, Benzoate, and Mixed Esters: Exploring Their Potential as Enzyme Immobilization Platforms

  2025cites this paper
• Biotransformation of Progesterone by Penicillium paxilli Isolated from Bertholletia excelsa Nuts: Immobilization on Silk Fibroin and Chitin

  2025cites this paper
• Functional modification of silk fibroin from silkworms and its application to medical biomaterials: A review.

  2024cites this paper
• Anti-inflammatory potential of baicalein combined with silk fibroin protein in a zebrafish model (Danio rerio)

  2022cites this paper
• Development of an environmentally friendly formulation of silk �broin associated with fatty acid from Astrocaryum murumuru Mart. fat, effective against larvae of the Aedes aegypti vector

  2021cites this paper
• In vitro anthelminthic activity of Lippia  alba essential oil combined with silk fibroin against monogeneans of Colossoma   macropomum (Serrasalmidae)

  2021cites this paper
• Taguchi design-assisted Co-immobilization of Lipase A and B from Candida antarctica onto Chitosan: characterization, kinetic resolution application, and docking studies

  2021cites this paper
• Silk: history, obtaining, structure and properties of an old material in the development of new technologies

  2021cites this paper
• Silk Fibroin-Based Materials for Catalyst Immobilization

  2020cites this paper
• Lipase mediated enzymatic kinetic resolution of phenylethyl halohydrins acetates: A case of study and rationalization

  2020cites this paper
• Larvicidal activity of the methanolic, hydroethanolic and hexanic extracts from Acmella oleracea, solubilized with silk fibroin, against Aedes aegypti

  2020cites this paper
• Immobilization of lipase onto metal–organic frameworks for enantioselective hydrolysis and transesterification

  2020cites this paper
• Synthesis of Chiral 1,3‐keto‐acetates through Enzymatic Kinetic Resolution with Amano Lipase from Pseudomonas fluorescens

  2020cites this paper
• Carapa guianensis Aubl. (Meliaceae) oil associated with silk fibroin, as alternative to traditional surfactants, and active against larvae of the vector Aedes aegypti

  2020cites this paper
• Silk materials for biotechnology

  2019cites this paper
• Chemoenzymatic Synthesis of Luliconazole Mediated by Lipases

  2018cites this paper
• Biotechnological potential of lipases from Pseudomonas: Sources, properties and applications

  2018cites this paper
• Stereoselective biocatalysis: A mature technology for the asymmetric synthesis of pharmaceutical building blocks*

  2018cites this paper