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REMI-seq: Development of methods and resources for functional genomics in Dictyostelium

Nicole Gruenheit,Amy Baldwin,Balint Stewart,Sarah Jaques,T. Keller,Katie Parkinson,R. Chisholm,A. Harwood,C. Thompson

Published 2019 in bioRxiv

ABSTRACT

Genomes can be sequenced with relative ease, but ascribing gene function remains a major challenge. Genetically tractable model systems are crucial to meet this challenge. One powerful model is the social amoeba Dictyostelium discoideum, a eukaryotic microbe widely used to study diverse questions in cell, developmental and evolutionary biology. However, its utility is hampered by the inefficiency with which sequence, transcriptome or proteome variation can be linked to phenotype. To address this, we have developed methods (REMI-seq) to (1) generate a near genome-wide resource of individual mutants (2) allow large-scale parallel phenotyping. We demonstrate that integrating these resources allows novel regulators of cell migration, phagocytosis and macropinocytosis to be rapidly identified. Therefore, these methods and resources provide a step change for high throughput gene discovery in a key model system, and the study of genes affecting traits associated with higher eukaryotes.

PUBLICATION RECORD

  • Publication year

    2019

  • Venue

    bioRxiv

  • Publication date

    2019-03-19

  • Fields of study

    Biology, Environmental Science

  • Identifiers
    DOI 10.1101/582072
  • External record

    Open on Semantic Scholar

  • Source metadata

    Semantic Scholar

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