Survival and function of isolated hepatocytes after cryopreservation.

No abstract is available for this paper.

• Publication year

  1999

• Venue

  Chemico-Biological Interactions

• Publication date

  1999-06-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/S0009-2797(99)00087-3PMID 10418967
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Effects of administration of the chemoprotective agent oltipraz on CYP1A and CYP2B in rat liver and rat hepatocytes in culture.

  1997cited by this paper
• Long-term primary cultures of adult human hepatocytes.

  1997cited by this paper
• Toxicity of allyl alcohol in primary cultures of freshly isolated and cryopreserved hepatocytes maintained on hydrated collagen gels.

  1997cited by this paper
• Long-term maintenance of drug-metabolizing enzyme activities in rat hepatocytes after cryopreservation.

  1997cited by this paper
• Influence of alginate gel entrapment and cryopreservation on survival and xenobiotic metabolism capacity of rat hepatocytes.

  1996cited by this paper
• Increase of cytochrome P-450 1A and glutathione transferase transcripts in cultured hepatocytes from dogs, monkeys, and humans after cryopreservation

  1996cited by this paper
• Report on the international workshop on the use of human in vitro liver preparations to study drug metabolism in drug development. Held at Utrecht, The Netherlands, 6-8 September 1994.

  1995cited by this paper
• Effective cryopreservation and long-term storage of primary human hepatocytes with recovery of viability, differentiation, and replicative potential.

  1995cited by this paper
• Xenobiotic metabolizing enzyme activities and viability are well preserved in EDTA-isolated rat liver parenchymal cells after cryopreservation.

  1995cited by this paper
• Report on the international workshop on the use of human in vitro liver preparations to study drug metabolism in drug development. Held at Utrecht, The Netherlands, 6-8 September 1994.

  1995cited by this paper
• Early clinical experience with a hybrid bioartificial liver.

  1995cited by this paper
• Cryopreservation of human adult hepatocytes for use in drug metabolism and toxicity studies.

  1993cited by this paper
• Evolution of the bioartificial liver: the need for randomized clinical trials.

  1993cited by this paper
• Preservation of the rate and profile of xenobiotic metabolism in rat hepatocytes stored in liquid nitrogen.

  1993cited by this paper
• CRYOPRESERVED MICROENCAPSULATED HEPATOCYTES—TRANSPLANTATION STUDIES IN GUNN RATS

  1993cited by this paper
• Viability and function in primary culture of adult hepatocytes from various animal species and human beings after cryopreservation

  1993cited by this paper
• Characterization of cryopreserved rat liver parenchymal cells by metabolism of diagnostic substrates and activities of related enzymes.

  1992cited by this paper
• Thawed human hepatocytes in primary culture.

  1992cited by this paper
• Development of an optimal method for the cryopreservation of hepatocytes and their subsequent monolayer culture.

  1991cited by this paper
• Insights into the cryoprotective mechanism of dimethyl sulfoxide for phospholipid bilayers.

  1991cited by this paper
• Intracellular Ice Formation during the Freezing of Hepatocytes Cultured in a Double Collagen Gel

  1991cited by this paper
• Development of bioartificial liver: bilirubin conjugation in Gunn rats.

  1990cited by this paper
• Cryopreservation of cynomolgus monkey (Macaca fascicularis) hepatocytes for subsequent culture and protein synthesis studies

  1990cited by this paper
• Auxiliary liver by transplanted frozen-thawed hepatocytes.

  1990cited by this paper
• A new approach to the cryopreservation of hepatocytes in a sandwich culture configuration.

  1990cited by this paper
• Optimization of cryopreservation procedures for rat and human hepatocytes.

  1989cited by this paper
• Primary culture of cryopreserved adult human hepatocytes on homologous extracellular matrix and the influence of monocytic products on albumin synthesis.

  1988cited by this paper
• Cryopreservation of isolated rat hepatocytes: a critical evaluation of freezing and thawing conditions.

  1988cited by this paper
• Cryopreservation of rat and dog hepatocytes for studies of xenobiotic metabolism and activation.

  1987cited by this paper
• Cryopreservation of adult human hepatocytes. The influence of deep freezing storage on the viability, cell seeding, survival, fine structures and albumin synthesis in primary cultures.

  1986cited by this paper
• Biochemical functionality and recovery of hepatocytes after deep freezing storage

  1984cited by this paper

• Cryopreservation of sturgeon egg mitochondria and their replacement in germline: A novel strategy for maternal genetic preservation in sturgeons.

  2025cites this paper
• Supercooled preservation of cultured primary rat hepatocyte monolayers

  2024cites this paper
• Comparison of cell viability methods for human mesenchymal/stromal stem cells and human A549 lung carcinoma cells after freeze-thaw stress.

  2023cites this paper
• [Analysis of protective mechanism of silk protein based cryoprotectants].

  2019cites this paper
• Transplante Hepatocitário: uma alternativa terapêutica para a insuficiência hepática aguda

  2019cites this paper
• Bulk Droplet Vitrification: An Approach to Improve Large-Scale Hepatocyte Cryopreservation Outcome.

  2018cites this paper
• Serum-Free Cryopreservation of Primary Rat Hepatocytes in a Modified Cold Storage Solution: Improvement of Cell Attachment and Function

  2018cites this paper
• Mitochondrial Impairment as a Key Factor for the Lack of Attachment after Cold Storage of Hepatocyte Suspensions

  2017cites this paper
• Cryopreservation of rat hepatocytes with disaccharides for cell therapy.

  2017cites this paper
• Optimisation of in vitro methodology for drug metabolism studies to improve prediction of hepatic drug clearance

  2016influential citation
• Cryopreservation of Hepatocytes.

  2015cites this paper
• Polyethylene glycol protects primary hepatocytes during supercooling preservation.

  2015cites this paper
• Biobanking: The Future of Cell Preservation Strategies.

  2015cites this paper
• Immortalization of Human Fetal Hepatocyte by Ectopic Expression of Human Telomerase Reverse Transcriptase, Human Papilloma Virus (E7) and Simian Virus 40 Large T (SV40 T) Antigen Towards Bioartificial Liver Support.

  2014cites this paper
• Study of the toxicological interactions between amphetamine designer drugs in the context of polydrug abuse

  2013cites this paper
• Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME

  2013cites this paper
• Cryopreserved mouse pancreatic acinar cells from long-term explant outgrowth cultures maintain their secretory phenotype after thawing.

  2013cites this paper
• Evaluating the Effects of Dithiothreitol and Fructose on Cell Viability and Function of Cryopreserved Primary Rat Hepatocytes and HepG2 Cell Line

  2013cites this paper
• Improvement of the Cold Storage of Isolated Human Hepatocytes

  2012cites this paper
• Development of optimised cryopreservation protocol for encapsulated liver cell spheroids : towards delivery of a bioartificial liver

  2012cites this paper
• Influence of cell culture configuration on the post-cryopreservation viability of primary rat hepatocytes.

  2012cites this paper
• Studies of human and porcine hepatocyte cryopreservation and their suitability for use in bioartificial liver development

  2012cites this paper
• Development of a modified vitrification strategy suitable for subsequent scale-up for hepatocyte preservation.

  2012influential citation
• Cryopreservation of Encapsulated Liver Spheroids for a Bioartificial Liver: Reducing Latent Cryoinjury Using an Ice Nucleating Agent

  2011cites this paper
• Cell Preservation Technology

  2011cites this paper
• Effect of black seed on dextromethorphan O- and N-demethylation in human liver microsomes and healthy human subjects.

  2010cites this paper
• Novel Cryopreservation Strategies for Cell‐Therapies and Pre‐Clinical Research

  2010cites this paper
• Optimisation of the cryopreservation of primary hepatocytes.

  2010cites this paper
• An improved purification approach with high cell viability and low cell loss for cryopreserved hepatocytes.

  2010cites this paper
• Studies on engineered microenvironment for manipulating functional cells in tissue regeneration

  2009cites this paper
• Effect of dithiotheritol on viability of cryopreserved rat hepatocytes

  2009cites this paper
• Preservation of Porcine Hepatocytes in Three-Dimensional Bioreactor at Room Temperature Using Epigallocatechin-3-Gallate

  2009cites this paper
• The use of vitrification to preserve primary rat hepatocyte monolayer on collagen-coated poly(ethylene-terephthalate) surfaces for a hybrid liver support system.

  2009cites this paper
• Cryopreservation in situ of cell monolayers on collagen vitrigel membrane culture substrata: ready-to-use preparation of primary hepatocytes and ES cells.

  2009cites this paper
• ADME optimization and toxicity assessment in early- and late-phase drug discovery.

  2009cites this paper
• Cryopreservation in Situ of Cell Monolayers on Collagen Vitrigel Membrane Culture Substrata: Ready-to-Use Preparation of Primary Hepatocytes and ES Cells

  2009cites this paper
• In vitro cultivation and cryopreservation of duck embryonic hepatocytes.

  2009cites this paper
• Vitrification Successfully Preserves Hepatocyte Spheroids

  2008cites this paper
• Peritoneal implantation of cryopreserved encapsulated porcine hepatocytes in rats without immunosuppression: viability and function.

  2008cites this paper
• Metabolic Activity of Cytochrome P450 Isoforms in Hepatocytes Cryopreserved with Wheat Protein Extract

  2008cites this paper
• A Paradigm Shift in Cryopreservation: Molecular-Based Advances to Improve Outcome

  2007cites this paper
• An Optimized Automated Assay for Determination of Metabolic Stability Using Hepatocytes: Assay Validation, Variance Component Analysis, and In Vivo Relevance

  2007cites this paper
• Hepatocyte transplantation: a review of laboratory techniques and clinical experiences.

  2007cites this paper
• Vitreous Cryopreservation of Cell–Biomaterial Constructs Involving Encapsulated Hepatocytes

  2007cites this paper
• FOR THE DEGREE CANDIDATA PHARMACIAE CYP 3 A 5 MEDIATED METABOLISM OF MIDAZOLAM IN VITRO

  2007cites this paper
• Cryopreservation of hepatocytes -Is it effective to cryopreserved hepatocytes using a new cryopreservation procedure combining a bioartificial approach? -

  2007cites this paper
• Present and Future Developments in Hepatic Tissue Engineering for Liver Support Systems

  2006cites this paper
• Improvement of Hepatocyte Viability after Cryopreservation by Supplementation of Long-Chain Oligosaccharide in the Freezing Medium in Rats and Humans

  2006cites this paper
• Primary mouse hepatocytes for systems biology approaches: a standardized in vitro system for modelling of signal transduction pathways.

  2006cites this paper
• Hepatocytes in suspension.

  2006cites this paper
• Wheat extracts as an efficient cryoprotective agent for primary cultures of rat hepatocytes

  2006cites this paper
• Immobilization of Cryopreserved Primary Rat Hepatocytes for the Development of a Bioartificial Liver System

  2005cites this paper
• Mild Hypothermic Preservation for Transport Purposes of the AMC Bioartificial Liver Charged with Porcine Hepatocytes

  2005cites this paper
• The Effect of Antioxidants and a Caspase Inhibitor on Cryopreserved Rat Hepatocytes

  2005cites this paper
• Non-Freezing Preservation and Subsequent Recovery of Hepatocytes Under PO2- and pH-Controlled Conditions for Continuous Supply of Cells for Bioartificial Liver Support

  2005cites this paper
• Phase I and II metabolism and carbohydrate metabolism in cultured cryopreserved porcine hepatocytes.

  2005cites this paper
• Etudes in vitro et in vivo du métabolisme du mycophénolate et des interactions métaboliques entre médicaments immunosuppresseurs

  2005cites this paper
• Cryopreservation and gel collagen culture of porcine hepatocytes.

  2004influential citation
• Metabolism of propafenone and verapamil by cryopreserved human, rat, mouse and dog hepatocytes: comparison with metabolism in vivo

  2004cites this paper
• Vitrification of encapsulated hepatocytes with reduced cooling and warming rates.

  2004cites this paper
• Cryopreservation of hepatocytes: a review of current methods for banking

  2004cites this paper
• Development of a protocol for cryopreservation of hepatocytes for use in bioartificial liver systems.

  2004influential citation
• Optimization of cryoprotectants for cryopreservation of rat hepatocyte

  2004cites this paper
• Improved techniques of organ preservation in transplantation.

  2004cites this paper
• Drug Metabolism In Preclinical Development

  2004cites this paper
• Evaluating the use of cryopreserved hepatocytes for the prediction of in vivo hepatic clearance

  2004cites this paper
• Studies into the isolation and cryopreservation of human and porcine hepatocytes and their suitability for use in bioartificial liver development

  2004cites this paper
• Apoptotic Cell Death and Function of Cryopreserved Porcine Hepatocytes in a Bioartificial Liver

  2003cites this paper
• Human hepatocytes as a tool for studying toxicity and drug metabolism.

  2003cites this paper
• Modulation of Pro-apoptotic (Bax) and Anti-apoptotic (Bcl-2) Gene Expression in Isolated Porcine Hepatocytes Perfused within a Radial-flow Bioreactor after Low-temperature Storing

  2003cites this paper
• In search of models for hepatic and placental pharmacokinetics

  2003cites this paper
• Strategies and Molecular Probes to Investigate the Role of Cytochrome P450 in Drug Metabolism

  2003cites this paper
• Utility of hepatocytes in predicting drug metabolism: comparison of hepatic intrinsic clearance in rats and humans in vivo and in vitro.

  2003cites this paper
• Effect of DMSO concentrations on cryopreservation of suckling porcine hepatocytes

  2002cites this paper
• Use of long-term cultures of human hepatocytes to study cytochrome P450 gene expression.

  2002cites this paper
• Hepatocyte cryopreservation in a three-dimensional structure.

  2002cites this paper
• Characterisation of primary porcine hepatocyte culture systems for use in bioartificial liver devices

  2002cites this paper
• Which hepatocyte will it be? Hepatocyte choice for bioartificial liver support systems

  2001cites this paper
• Cryopreservation of suckling pig hepatocytes.

  2001cites this paper
• OPTIMISATION OF PORCINE HEPATOCYTE CRYOPRESERVATION BY COMPARISON OF VIABILITY AND ENZYMATIC ACTIVITY OF FRESH AND CRYOPRESERVED CELLS

  2001cites this paper
• Semiautomatic Macroencapsulation of Fresh or Cryopreserved Porcine Hepatocytes Maintain Their Ability for Treatment of Acute Liver Failure

  2001cites this paper
• Woodchuck hepatocytes remain permissive for hepadnavirus infection and mouse liver repopulation after cryopreservation

  2001cites this paper
• Preparation of Hepatocytes

  2001cites this paper
• OPTIMISATION OF PORCINE HEPATOCYTE CRYOPRESERVATION BY COMPARISON OF VIABILITY AND ENZYMATIC ACTIVITY OF FRESH AND CRYOPRESERVED CELLS

  2001cites this paper
• Oxygen transfer in a convection-enhanced hollow fiber bioartificial liver.

  2001cites this paper
• Caspase inhibition reduces apoptotic death of cryopreserved porcine hepatocytes

  2001cites this paper
• Functional evaluation of the AMC-BAL to be employed in a multicentric clinical trial for acute liver failure.

  2001cites this paper
• Postthaw viability of precultured hepatocytes.

  2001cites this paper
• Influence of preculture on the prefreeze and postthaw characteristics of hepatocytes.

  2000cites this paper
• Present status of the application of cryopreserved hepatocytes in the evaluation of xenobiotics: consensus of an international expert panel.

  1999cites this paper