DnaJB6 is a RanGTP-regulated protein involved in dynein-dependent microtubule organization during mitosis

Bipolar spindle organization is essential for the faithful segregation of chromosomes during cell division. This organization relies on the collective activities of motor proteins. The minus-end directed dynein motor complex generates spindle inward forces and plays a major role in spindle pole focusing. The dynactin complex regulates many dynein functions increasing its processivity and force production. Here we show that DnaJB6 is a novel RanGTP regulated protein. It interacts with dynactin p150Glued in a RanGTP-dependent manner specifically in M-phase and promotes spindle pole focusing and dynein force generation. Our data suggest a novel mechanism by which RanGTP regulates dynein activity during M-phase. Summary statement We describe DnaJB6 as a novel RanGTP-regulated protein important for spindle assembly. Our data suggest that RanGTP regulates dynein-dependent inward spindle force generation and pole focusing through DnaJB6

• Publication year

  2019

• Venue

  bioRxiv

• Publication date

  2019-01-31

• Fields of study

  Biology, Chemistry

• Identifiers
  DOI 10.1101/536045
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Functional Analysis

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