Generation and analysis of expressed sequence tags from roots cDNA library of cotton (Gossypium arboreum)

Cotton is major fiber crop among agricultural commodities grown worldwide. Prolonged osmotic stress and seasonal water shortage effect plant growth and yields. To explore the drought responsive molecular mechanism in cotton roots, we constructed cDNA library for the identification, functional annotation and expression analysis of potential drought responsive transcripts. mRNA extracted from cotton roots under drought stress was used to construct cDNA library. A total of 711 expressed sequence tags were generated from cDNA library. NCBI BLAST analysis of reported expressed sequence tags showed homology of 27% sequences with Gossypium species while 24% sequences showed no sequence similarity in non-redundant nucleotide database. Remaining 49% sequences revealed sequence similarity results with other plant species. Functional annotation was performed for sequenced EST’s using Arabidopsis Gene ontology database (TAIR). We examined the expression pattern of 08 differentially expressed ESTs using RTqPCR and validated that all these were upregulated in roots under osmotic stress, but to different folds. The candidate EST sequences identified in this study will be valuable resource to elucidate stress responsive molecular mechanism and genetic breeding of cotton in future.

• Publication year

  2019

• Venue

  Pakistan journal of botany

• Publication date

  2019-04-10

• Fields of study

  Agricultural and Food Sciences, Biology

• Identifiers
  DOI 10.30848/PJB2019-4(14)
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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  2013influential reference
• GmNFYA3, a target gene of miR169, is a positive regulator of plant tolerance to drought stress

  2013cited by this paper
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  2012cited by this paper
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  2012cited by this paper
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  2012cited by this paper
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  2012cited by this paper
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  2012cited by this paper
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  2012cited by this paper
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  2011cited by this paper
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  2011cited by this paper
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  2011cited by this paper
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  2011cited by this paper
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  2011cited by this paper
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  2011cited by this paper
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  2010cited by this paper
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  2010cited by this paper
• Functional diversity of the plant glycine-rich proteins superfamily

  2010cited by this paper
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  2010cited by this paper
• Receptor-like kinases shape the plant

  2009cited by this paper
• Construction and analysis of cotton (Gossypium arboreum L.) drought-related cDNA library

  2009cited by this paper
• Transcript profiling during fiber development identifies pathways in secondary metabolism and cell wall structure that may contribute to cotton fiber quality.

  2009cited by this paper
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  2008cited by this paper
• Galactinol is a signaling component of the induced systemic resistance caused by Pseudomonas chlororaphis O6 root colonization.

  2008cited by this paper
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  2008cited by this paper
• The Arabidopsis NFYA5 Transcription Factor Is Regulated Transcriptionally and Posttranscriptionally to Promote Drought Resistance[W]

  2008cited by this paper
• Galactinol and Raffinose Constitute a Novel Function to Protect Plants from Oxidative Damage1[W][OA]

  2008cited by this paper
• Stomatal movements and long-distance signaling in plants

  2008cited by this paper
• Alterations in photosynthetic pigments, protein and osmotic components in cotton genotypes subjected to short-term drought stress followed by recovery

  2007cited by this paper
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  2007cited by this paper
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  2006cited by this paper
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  2006cited by this paper
• Functional analysis of receptor-like kinases in monocots and dicots.

  2006cited by this paper
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  2006cited by this paper
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  2006cited by this paper
• The CCAAT binding factor can mediate interactions between CONSTANS-like proteins and DNA.

  2006cited by this paper
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  2005cited by this paper
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  2005cited by this paper
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  2004cited by this paper
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  2004cited by this paper
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  2003cited by this paper
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  2003cited by this paper
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  2002cited by this paper
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• Base-calling of automated sequencer traces using phred. II. Error probabilities.

  1998cited by this paper
• Base-calling of automated sequencer traces using phred. I. Accuracy assessment.

  1998cited by this paper
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  1997cited by this paper
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