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Enzymatic degradation of azo dyes using three macrophyte species: Arundo donax, Typha angustifolia and Phragmites australis

Dalila Haddaji,L. Bousselmi,O. Saadani,I. Nouairi,Z. Ghrabi-Gammar

Published 2014 in Desalination and Water Treatment

ABSTRACT

AbstractThe aim of this study was to investigate the potential role of three macrophyte species (Arundo donax, Typha angustifolia and Phragmites australis) for degradation of azo dyes. Activities of peroxidases enzymes involved in plant protection against stress were tested for dye decolourization. In order to conduct the enzyme activity assay, the fresh extract was obtained from crude extracts of leaves. The oxidation capability of peroxidase (POD) on direct azo dyes (amaranth and amido black) was investigated and found to be an effective treatment methodology. The performance of peroxidase was evaluated in soluble and free form in the presence of H2O2. The oxidation was tested as a function of peroxidase at constant POD activity. Parameters such as temperature (5–70°C), concentration of H2O2 (0–1000 μM/L), pH (4–10), dye concentration (0.002–1 mM/L) and enzyme amount (5–20 μL) were studied. Results show that P. australis, leaves contained the highest peroxidase activity. The dye decolourization is about...

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