FTIR nanobiosensors for Escherichia coli detection

Infections due to enterohaemorrhagic E. coli (Escherichia coli) have a low incidence but can have severe and sometimes fatal health consequences, and thus represent some of the most serious diseases due to the contamination of water and food. New, fast and simple devices that monitor these pathogens are necessary to improve the safety of our food supply chain. In this work we report on mesoporous titania thin-film substrates as sensors to detect E. coli O157:H7. Titania films treated with APTES ((3-aminopropyl)triethoxysilane) and GA (glutaraldehyde) were functionalized with specific antibodies and the absorption properties monitored. The film-based biosensors showed a detection limit for E. coli of 1 × 102 CFU/mL, constituting a simple and selective method for the effective screening of water samples.

• Publication year

  2012

• Venue

  Beilstein Journal of Nanotechnology

• Publication date

  2012-07-03

• Fields of study

  Biology, Materials Science, Chemistry, Environmental Science, Medicine

• Identifiers
  DOI 10.3762/bjnano.3.55PMID 23019542PMCID 3458592
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Biosensors as innovative tools for the detection of food borne pathogens.

  2011cited by this paper
• Microbial biosensors: a review.

  2011cited by this paper
• Nanomaterials based biosensors for food analysis applications

  2011cited by this paper
• Polypeptide binding to mesostructured titania films

  2011cited by this paper
• Int J Food Microbiol

  2011cited by this paper
• An overview of foodborne pathogen detection: in the perspective of biosensors.

  2010cited by this paper
• An Amperometric Immunosensor for IgG Based on Conducting Polymer and Carbon Nanotube‐Linked Hydrazine Label

  2010cited by this paper
• Application of segmented flow for quality control of food using microfluidic tools

  2010cited by this paper
• Biofunctionalized magnetic nanoparticle integrated mid-infrared pathogen sensor for food matrixes.

  2009cited by this paper
• Detection of Escherichia coli O157:H7, Salmonella typhimurium, and Legionella pneumophila in water using a flow-through chemiluminescence microarray readout system.

  2008cited by this paper
• Rapid quantification of bacterial cells in potable water using a simplified microfluidic device.

  2007cited by this paper
• Pathogen detection: a perspective of traditional methods and biosensors.

  2007cited by this paper
• Construction and evaluation of a microbiological positive process internal control for PCR-based examination of food samples for Listeria monocytogenes and Salmonella enterica.

  2007cited by this paper
• A label-free, microfluidics and interdigitated array microelectrode-based impedance biosensor in combination with nanoparticles immunoseparation for detection of Escherichia coli O157:H7 in food samples

  2007cited by this paper
• An optical biosensor for rapid and label-free detection of cells.

  2006cited by this paper
• Detection of Salmonella in environmental water and sediment by a nested-multiplex polymerase chain reaction assay.

  2005cited by this paper
• Detection of pathogenic bacteria in food samples using highly-dispersed carbon particles.

  2005cited by this paper
• Evaluation of a monoclonal antibody-based enzyme-linked immunosorbent assay for detection of Campylobacter fetus in bovine preputial washing and vaginal mucus samples.

  2004cited by this paper
• DIFFERENTIATION OF MICROORGANISMS BY FTIR-ATR AND NIR SPECTROSCOPY

  2004cited by this paper
• Advances in biosensors for detection of pathogens in food and water

  2003cited by this paper
• Market analysis of biosensors for food safety.

  2003cited by this paper
• Review of the use of biosensors as analytical tools in the food and drink industries

  2002cited by this paper
• An antibody-immobilized capillary column as a bioseparator/bioreactor for detection of Escherichia coil O157:H7 with absorbance measurement.

  2001cited by this paper
• A review of molecular recognition technologies for detection of biological threat agents.

  2000cited by this paper
• Methodology for detection and typing of foodborne microorganisms.

  1999cited by this paper
• Flow-through immunofiltration assay system for rapid detection of E. coli O157:H7.

  1999cited by this paper
• Biosensors for detection of pathogenic bacteria

  1999cited by this paper
• Foodborne Disease Significance of Escherichia coli O157:H7 and Other Enterohemorrhagic E. coli

  1997cited by this paper
• Epidemiology of hemolytic-uremic syndrome in Canadian children from 1986 to 1988. The Canadian Pediatric Kidney Disease Reference Centre.

  1991cited by this paper

• Surface chemical modification of poly(dimethylsiloxane) for stabilizing antibody immobilization and T cell cultures.

  2024cites this paper
• Real-time Detection and Analysis of Foodborne Pathogens via Machine Learning based Fiber Optic Raman Sensor

  2023cites this paper
• Printable biosensors towards next-generation point-of-care testing: paper substrate as an example.

  2023cites this paper
• Novel electrochemical biosensor for Escherichia coli using gold-coated tungsten wires and antibody functionalized short multiwalled carbon nanotubes

  2023cites this paper
• Efficient classification of Escherichia coli and Shigella using FT-IR spectroscopy and multivariate analysis.

  2022cites this paper
• An In Vitro Study of the Antifungal Efficacy of Zinc Oxide Nanoparticles against Saccharomyces cerevisiae

  2022cites this paper
• Conjugation of E.coli antibody with fluorescent natural silica-based nanoparticles: Preparation and characterization

  2021cites this paper
• Interdigitated Electrode Biosensor Based on Plasma-Deposited TiO2 Nanoparticles for Detecting DNA

  2021cites this paper
• Optimization and Characterization of Immobilized Laccase on Titanium Dioxide Nanostructure and its Application in Removal of Remazol Brilliant Blue R

  2021cites this paper
• Characterisation and Classification of Foodborne Bacteria Using Reflectance FTIR Microscopic Imaging

  2021cites this paper
• Application of Nanobiosensor in Health Care Sector

  2021cites this paper
• Probing Nanoscale Interactions of Antimicrobial Zinc Oxide Quantum Dots on Bacterial and Fungal Cell Surfaces

  2021cites this paper
• Antibody Immobilization in Zinc Oxide Thin Films as an Easy-Handle Strategy for Escherichia coli Detection

  2020cites this paper
• Applications of nanotechnology on vegetable crops.

  2020cites this paper
• Klinik İzolatların Genişlemiş Spektrumlu Beta Laktamaz Üretimi Profillerinin Belirlenmesi ve Hücre Bileşenlerinin FTIR İle Tespiti

  2020cites this paper
• Effects of Ultrafine-bubbles on gram-negative bacteria: Inhibition or Selection?

  2019cites this paper
• Advanced Nanoparticle-Based Biosensors for Diagnosing Foodborne Pathogens

  2019cites this paper
• Label-free amperometric biosensor for Escherichia coli O157:H7 detection

  2019cites this paper
• Mesoporous TiO2 Thin Films: State of the Art

  2018cites this paper
• Modified surface of titanium dioxide nanoparticles-based biosensor for DNA detection

  2018cites this paper
• Análisis y estudio de la interacción de escherichia coli ETEC E9034A con carburo de silicio amorfo hidrogenado como soporte inmovilizador

  2018influential citation
• Biofunctionalization Process of a -SiC:H Surfaces Applied to an Interdigitated Microelectrode Array to Detect Enterotoxigenic Escherichia coli

  2017cites this paper
• Borosilicate Glass Fiber-Optic Biosensor for the Detection of Escherichia coli

  2017cites this paper
• Adhesion resistant chitosan coated iron oxide polyacrylonitrile mixed matrix membrane for disinfection of surface water

  2017cites this paper
• Comparative CO2 Adsorption Analysis in Pure and Amine-Modified Composite Membranes

  2017cites this paper
• Applying graphene oxide nano-film over a polycarbonate nanoporous membrane to monitor E. coli by infrared spectroscopy.

  2017cites this paper
• Interdigitated microelectrode geometry for simple electrical Escherichia coli O157:H7 DNA detection

  2017cites this paper
• Biosensors for the detection of Escherichia coli

  2017influential citation
• International Journal of Materials Science and Engineering

  2016cites this paper
• Fourier transform infrared spectroscopy to assess molecular-level changes in microorganisms exposed to nanoparticles

  2016cites this paper
• Water monitoring with hyperspectral techniques

  2015cites this paper
• Design, fabrication and performance evaluation of interdigital capacitive sensor for detection of Cardiac Troponin-I and Human Epidermal Growth Factor Receptor 2

  2015cites this paper
• Surface modification of silicon dioxide, silicon nitride and titanium oxynitride for lactate dehydrogenase immobilization.

  2015cites this paper
• Construction Of Anti Omp Immobilized Nanoporous Membrane Based Electrochemical Biosensor For The Detection Of E. Coli

  2015cites this paper
• Low-cost, portable biosensors for Escherichia coli detection in water

  2015cites this paper
• Sensitivity analysis of the detection of Ganoderma boninense infection in oil palm using FTIR

  2014cites this paper
• Immobilization of antibodies and enzymes on 3-aminopropyltriethoxysilane-functionalized bioanalytical platforms for biosensors and diagnostics.

  2014cites this paper
• Biomolecular modification of zirconia surfaces for enhanced biocompatibility

  2014cites this paper
• Current and Future Trends in the Clinical Diagnosis of Rickettsioses Sensu Lato

  2014cites this paper
• Nanotechnology and its role in pathogen detection: A short review

  2014cites this paper
• Nano/micro and spectroscopic approaches to food pathogen detection.

  2014cites this paper
• Tracing Emerging Irreversibilities and Development Pathways in the Field of Nanosensor Technologies

  2013cites this paper
• Mesoporous thin films: properties and applications.

  2013cites this paper
• Nanobiosensors: Diagnostic Tool for Pathogen Detection

  2013cites this paper
• Design of a core–shell type immuno-magnetic separation system and multiplex PCR for rapid detection of pathogens from food samples

  2013cites this paper
• Advances of nanotechnology in agro-environmental studies

  2013cites this paper
• Novel planar interdigital sensors for the detection of bacterial endotoxins : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philososphy in Electronics Engineering at Massey University, Palmerston North, New Zealand

  2012cites this paper
• An In Vitro Study of the Antifungal Efficacy of Zinc Oxide Nanoparticles against Saccharomyces cerevisiae

  year unknowncites this paper