Aequorin-based measurements of intracellular Ca2+-signatures in plant cells

Due to the involvement of calcium as a main second messenger in the plant signaling pathway, increasing interest has been focused on the calcium signatures supposed to be involved in the patterning of the specific response associated to a given stimulus. In order to follow these signatures we described here the practical approach to use the non-invasive method based on the aequorin technology. Besides reviewing the advantages and disadvantages of this method we report on results showing the usefulness of aequorin to study the calcium response to biotic (elicitors) and abiotic stimuli (osmotic shocks) in various compartments of plant cells such as cytosol and nucleus.

• Publication year

  2002

• Venue

  Biological Procedures Online

• Publication date

  2002-05-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1251/bpo40PMID 12734562PMCID 145563
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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