Effect of the medium composition on formation of amylase by Bacillus sp

Studies on the a -amylase synthesis was carried out with a moderately thermophilic, facultatively anaerobic Bacillus sp, isolated from soil samples. The cells were cultivated in a complex medium containing soluble starch or maltose as carbon source. The levels of the a -amylaseactivity detected in culture supernatants varied greatly with the type of carbon source used. Maltose, soluble starch and citrate stimulated a -amylaseformation. Addition of exogenous glucose repressed formation of a -amylase, demonstrating that a classical glucose effect was operative in this organism. The concentration of yeast extract was found to be important factor in the a -amylase synthesis bythe isolate.The activity of the enzyme increased between 2 and 5 g/L yeast extract concentration and then fell very rapidly beyond this point. The best concentration of peptone to a-amylase formation was found to be around 10g/L.

• Publication year

  2003

• Venue

  Brazilian Archives of Biology and Technology

• Publication date

  Unknown publication date

• Fields of study

  Biology, Environmental Science

• Identifiers
  DOI 10.1590/S1516-89132003000100018
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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