4-Demethylwyosine Synthase from Pyrococcus abyssi Is a Radical-S-adenosyl-l-methionine Enzyme with an Additional [4Fe-4S]+2 Cluster That Interacts with the Pyruvate Co-substrate*

No abstract is available for this paper.

• Publication year

  2012

• Venue

  Journal of Biological Chemistry

• Publication date

  2012-10-05

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1074/jbc.M112.405019PMID 23043105PMCID PMC3510817
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The radical SAM enzyme AlbA catalyzes thioether bond formation in subtilosin A.

  2012cited by this paper
• Studies of inhibitor binding to the [4Fe-4S] cluster of quinolinate synthase.

  2012cited by this paper
• Biosynthesis of isoprene units: Mössbauer spectroscopy of substrate and inhibitor binding to the [4Fe-4S] cluster of the LytB/IspH enzyme.

  2011cited by this paper
• Pyruvate is the source of the two carbons that are required for formation of the imidazoline ring of 4-demethylwyosine.

  2011influential reference
• A consensus mechanism for Radical SAM-dependent dehydrogenation? BtrN contains two [4Fe-4S] clusters.

  2010cited by this paper
• Biosynthesis of wyosine derivatives in tRNA: an ancient and highly diverse pathway in Archaea.

  2010cited by this paper
• Isoprenoid biosynthesis via the MEP pathway: in vivo Mössbauer spectroscopy identifies a [4Fe-4S]2+ center with unusual coordination sphere in the LytB protein.

  2009cited by this paper
• DNA and RNA Modification Enzymes: Structure, Mechanism, Function and Evolution

  2009cited by this paper
• ENDOR spectroscopy shows that guanine N1 binds to [4Fe-4S] cluster II of the S-adenosylmethionine-dependent enzyme MoaA: mechanistic implications.

  2009cited by this paper
• Expanding chemical biology of 2-oxoglutarate oxygenases.

  2008cited by this paper
• Structure of an archaeal TYW1, the enzyme catalyzing the second step of wye-base biosynthesis.

  2007cited by this paper
• Crystal structure of the radical SAM enzyme catalyzing tricyclic modified base formation in tRNA.

  2007influential reference
• Characterization of the DNA repair spore photoproduct lyase enzyme from Clostridium acetobutylicum : A radical-SAM enzyme

  2007cited by this paper
• Specific correlation between the wobble modification deficiency in mutant tRNAs and the clinical features of a human mitochondrial disease.

  2005cited by this paper
• Discovery of a Gene Family Critical to Wyosine Base Formation in a Subset of Phenylalanine-specific Transfer RNAs*

  2005cited by this paper
• Human Mitochondrial Diseases Associated with tRNA Wobble Modification Deficiency

  2005cited by this paper
• Activation of the Anaerobic Ribonucleotide Reductase by S‐Adenosylmethionine

  2005cited by this paper
• S-adenosylmethionine: nothing goes to waste.

  2004cited by this paper
• Coordination and mechanism of reversible cleavage of S-adenosylmethionine by the [4Fe-4S] center in lysine 2,3-aminomutase.

  2003cited by this paper
• The [4Fe-4S](2+) cluster in reconstituted biotin synthase binds S-adenosyl-L-methionine.

  2002cited by this paper
• Radical SAM, a novel protein superfamily linking unresolved steps in familiar biosynthetic pathways with radical mechanisms: functional characterization using new analysis and information visualization methods.

  2001cited by this paper
• Characterization of Bimodal Coordination Structure in Nitrosyl Heme Complexes through Hyperfine Couplings with Pyrrole and Protein Nitrogens

  1999cited by this paper
• The importance of being modified: roles of modified nucleosides and Mg2+ in RNA structure and function.

  1996influential reference
• Summary: the modified nucleosides of RNA.

  1994cited by this paper
• Ribonucleoside analysis by reversed-phase high-performance liquid chromatography.

  1989influential reference
• Rapid colorimetric micromethod for the quantitation of complexed iron in biological samples.

  1988cited by this paper
• Mössbauer and EPR studies of activated aconitase: development of a localized valence state at a subsite of the [4Fe-4S] cluster on binding of citrate.

  1983cited by this paper
• Semi-micro methods for analysis of labile sulfide and of labile sulfide plus sulfane sulfur in unusually stable iron-sulfur proteins.

  1983cited by this paper
• Structure of wye (Yt base) and wyosine (Yt) from Torulopsis utilis phenylalanine transfer ribonucleic acid.

  1976cited by this paper

• Nitrogen-centered radicals driving unusual enzyme reactions in biosynthetic pathways.

  2025cites this paper
• Spectroscopic and Biochemical Characterization of the Noncanonical Radical SAM Enzyme ArsL, Involved in Arsinothricin Biosynthesis.

  2025cites this paper
• Substrate Specificity of a Methyltransferase Involved in the Biosynthesis of the Lantibiotic Cacaoidin

  2024cites this paper
• Insertion of 4-Demethylwyosine in tRNAPhe Catalyzed by the Radical S-Adenosyl-l-methionine Enzyme TYW1 Entails Oxidative Cleavage of Pyruvate to Form CO2.

  2022cites this paper
• Iron in Translation: From the Beginning to the End

  2021cites this paper
• Eukaryotic TYW1 Is a Radical SAM Flavoenzyme

  2021cites this paper
• Etheno adducts: from tRNA modifications to DNA adducts and back to miscoding ribonucleotides

  2021cites this paper
• Redox Mediated Modifications of tRNA Bases

  2020cites this paper
• 1-Biosynthesis of sulfur-and selenium-containing nucleosides

  2020cites this paper
• Iron-sulfur proteins in plant mitochondria: roles and maturation.

  2020cites this paper
• Radical-Mediated Carbon Skeleton Formation in Cofactor and Natural Product Biosynthesis

  2019cites this paper
• Structural studies of S-adenosyl-L-methionine radical enzymes involved in tRNA and natural product biosynthesis

  2018cites this paper
• Biochemical and Structural Characterization of a Schiff Base in the Radical-Mediated Biosynthesis of 4-Demethylwyosine by TYW1

  2018cites this paper
• Formation and Cleavage of C-C Bonds by Enzymatic Oxidation-Reduction Reactions.

  2018cites this paper
• A Radical Clock Probe Uncouples H Atom Abstraction from Thioether Cross-Link Formation by the Radical S-Adenosyl-l-methionine Enzyme SkfB

  2018cites this paper
• TYW1: A Radical SAM Enzyme Involved in the Biosynthesis of Wybutosine Bases.

  2018cites this paper
• Transfer RNA Modification Enzymes from Thermophiles and Their Modified Nucleosides in tRNA

  2018cites this paper
• Identification of Steps in the Pathway of Arsenosugar Biosynthesis.

  2018cites this paper
• Eight Kinetically Stable but Thermodynamically Activated Molecules that Power Cell Metabolism.

  2017cites this paper
• Characterization of auxiliary iron–sulfur clusters in a radical S‐adenosylmethionine enzyme PqqE from Methylobacterium extorquens AM1

  2017cites this paper
• On the Role of Additional [4Fe-4S] Clusters with a Free Coordination Site in Radical-SAM Enzymes

  2017cites this paper
• Flavin-dependent epitranscriptomic world.

  2017cites this paper
• as Terminal Electron Acceptor : From TCA Cycle to Cytochrome Oxidase V 9 . 2 . Iron-Based Oxygenases

  2017cites this paper
• Spectroscopic evidence for cofactor-substrate interaction in the radical-SAM enzyme TYW1.

  2017cites this paper
• Genetic, Biochemical, and Biophysical Methods for Studying FeS Proteins and Their Assembly.

  2017cites this paper
• Characterization of Radical S-adenosylmethionine Enzymes and Intermediates in their Reactions by Continuous Wave and Pulse Electron Paramagnetic Resonance Spectroscopies

  2017cites this paper
• PqqE from Methylobacterium extorquens AM1: a radical S-adenosyl-l-methionine enzyme with an unusual tolerance to oxygen.

  2016cites this paper
• Structure of Quinolinate Synthase from Pyrococcus horikoshii in the Presence of Its Product, Quinolinic Acid.

  2016cites this paper
• Unanticipated coordination of tris buffer to the Radical SAM cluster of the RimO methylthiotransferase

  2016cites this paper
• Radical S-Adenosylmethionine Enzymes in Human Health and Disease.

  2016cites this paper
• Redox Behavior of the S-Adenosylmethionine (SAM)-Binding Fe-S Cluster in Methylthiotransferase RimO, toward Understanding Dual SAM Activity.

  2016cites this paper
• Mechanistic Studies of the Radical S-Adenosyl-L-methionine Enzyme 4-Demethylwyosine Synthase Reveal the Site of Hydrogen Atom Abstraction.

  2015cites this paper
• Iron-sulfur proteins responsible for RNA modifications.

  2015cites this paper
• A common tRNA modification at an unusual location: the discovery of wyosine biosynthesis in mitochondria

  2015cites this paper
• Design, Synthesis and Evaluation of Fe-S Targeted Adenosine 5′-Phosphosulfate Reductase Inhibitors

  2015cites this paper
• Auxiliary iron-sulfur cofactors in radical SAM enzymes.

  2015cites this paper
• Wybutosine biosynthesis: Structural and mechanistic overview

  2014cites this paper
• Study of metalloproteins using continuous wave electron paramagnetic resonance (EPR).

  2014cites this paper
• Biosynthesis of wyosine derivatives in tRNAPhe of Archaea: role of a remarkable bifunctional tRNAPhe:m1G/imG2 methyltransferase

  2014cites this paper
• Characterization of two unique pathways for wyosine biosynthesis in Kinetoplastids

  2014influential citation
• Fe–S Clusters: Biogenesis and Redox, Catalytic, and Regulatory Properties

  2014cites this paper
• Radical S-Adenosylmethionine Enzymes

  2014cites this paper
• Cysteine desulphurase plays an important role in environmental adaptation of the hyperthermophilic archaeon Thermococcus kodakarensis

  2014cites this paper
• Posttranscriptional RNA Modifications: playing metabolic games in a cell's chemical Legoland.

  2014influential citation
• Recent Advances in Radical SAM Enzymology: New Structures and Mechanisms

  2014cites this paper
• Methylated nucleosides in tRNA and tRNA methyltransferases

  2014cites this paper
• Biochemical and kinetic characterization of radical S-adenosyl-L-methionine enzyme HydG.

  2013cites this paper
• An EPR/HYSCORE, Mössbauer, and resonance Raman study of the hydrogenase maturation enzyme HydF: a model for N-coordination to [4Fe–4S] clusters

  2013cites this paper
• Radical mediated ring formation in the biosynthesis of the hypermodified tRNA base wybutosine.

  2013cites this paper
• Biochemical and kinetic characterization of radical AdoMet enzyme HydG

  year unknowncites this paper