A polymeric microfluidic device integrated with nanoporous alumina membranes for simultaneous detection of multiple foodborne pathogens

Abstract Conventional biochemical methods for foodborne pathogen detection are time-consuming, labor-intensive and can only be used for single type bacteria detection with one sample. There is always an urgent need for fast, accurate and easy to handle devices for identification and monitoring of multiple foodborne pathogens at the same time. Herein, we present a non-biofouling polyethylene glycol (PEG) based microfluidic chip integrated with functionalized nanoporous alumina membrane for simultaneous electrochemical detection of two types of bacteria Escherichia coli O157:H7 and Staphylococcus aureus from the mixed samples. The experimental results demonstrated the specificity for target bacteria detection and the low cross-binding of non-target bacteria. The simultaneous detection of mixed bacteria sample of E. coli O157:H7 and S. aureus was also demonstrated. This sensor has a linear detection range from 102 CFU/mL to 105 CFU/mL with the limit of detection (LOD) around 102 CFU/mL.

• Publication year

  2016

• Venue

  Sensors and Actuators B-chemical

• Publication date

  2016-03-31

• Fields of study

  Agricultural and Food Sciences, Materials Science, Engineering, Environmental Science

• Identifiers
  DOI 10.1016/J.SNB.2015.11.059
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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