Streptococcus pyogenes in Human Plasma

Background: The human pathogen Streptococcus pyogenes adapts to vascular leakage at the site of infection. Results: S. pyogenes modifies the production of 213 in plasma determined using quantitative proteomics. Conclusion: The results clarify the function of HSA-binding proteins in S. pyogenes. Significance: Our data demonstrates the power of the quantitative mass spectrometry strategy to investigate bacterial adaptation to a given environment. Streptococcus pyogenes is a major bacterial pathogen and a potent inducer of inflammation causing plasma leakage at the site of infection. A combination of label-free quantitative mass spectrometry-based proteomics strategies were used to measure how the intracellular proteome homeostasis of S. pyogenes is influenced by the presence of human plasma, identifying and quantifying 842 proteins. In plasma the bacterium modifies its production of 213 proteins, and the most pronounced change was the complete down-regulation of proteins required for fatty acid biosynthesis. Fatty acids are transported by albumin (HSA) in plasma. S. pyogenes expresses HSA-binding surface proteins, and HSA carrying fatty acids reduced the amount of fatty acid biosynthesis proteins to the same extent as plasma. The results clarify the function of HSA-binding proteins in S. pyogenes and underline the power of the quantitative mass spectrometry strategy used here to investigate bacterial adaptation to a given environment.

• Publication year

  2011

• Venue

  Journal of Biological Chemistry

• Publication date

  2011-11-23

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1074/jbc.M111.267674PMID 22117078PMCID PMC3256886
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• High-throughput generation of selected reaction-monitoring assays for proteins and proteomes

  2010cited by this paper
• The giant extracellular matrix‐binding protein of Staphylococcus epidermidis mediates biofilm accumulation and attachment to fibronectin

  2010cited by this paper
• Modern Applied Statistics with S

  2010cited by this paper
• Streptococcal M proteins and their role as virulence determinants.

  2010cited by this paper
• Binding of Albumin Promotes Bacterial Survival at the Epithelial Surface*

  2010cited by this paper
• Microevolution of Group A Streptococci In Vivo: Capturing Regulatory Networks Engaged in Sociomicrobiology, Niche Adaptation, and Hypervirulence

  2010cited by this paper
• Proteome-wide cellular protein concentrations of the human pathogen Leptospira interrogans

  2009cited by this paper
• Calculating absolute and relative protein abundance from mass spectrometry-based protein expression data.

  2008cited by this paper
• OpenMS – An open-source software framework for mass spectrometry

  2008cited by this paper
• Calculating absolute and relative protein abundance from mass spectrometry-based protein expression data

  2008cited by this paper
• Membrane lipid homeostasis in bacteria

  2008cited by this paper
• Targeted Quantitative Analysis of Streptococcus pyogenes Virulence Factors by Multiple Reaction Monitoring*S

  2008cited by this paper
• Absolute protein expression profiling estimates the relative contributions of transcriptional and translational regulation

  2007cited by this paper
• Characterization of Group A Streptococcal M23 Protein and Comparison of the M3 and M23 Protein’s Ligand-Binding Domains

  2007cited by this paper
• TOPP - the OpenMS proteomics pipeline

  2007cited by this paper
• Target-decoy search strategy for increased confidence in large-scale protein identifications by mass spectrometry

  2007cited by this paper
• Absolute Quantification of Proteins by LCMSE

  2006cited by this paper
• The contact system—a novel branch of innate immunity generating antibacterial peptides

  2006cited by this paper
• 2DDB – a bioinformatics solution for analysis of quantitative proteomics data

  2006cited by this paper
• The National Microbial Pathogen Database Resource (NMPDR): a genomics platform based on subsystem annotation

  2006cited by this paper
• Transcriptional regulation of fatty acid biosynthesis in Streptococcus pneumoniae

  2006cited by this paper
• Group A Streptococcus transcriptome dynamics during growth in human blood reveals bacterial adaptive and survival strategies.

  2005cited by this paper
• Real-time quantification of fatty acid uptake using a novel fluorescence assay Published, JLR Papers in Press, November 16, 2004. DOI 10.1194/jlr.D400023-JLR200

  2005cited by this paper
• Transcriptional control of membrane lipid synthesis in bacteria.

  2005cited by this paper
• Exponentially Modified Protein Abundance Index (emPAI) for Estimation of Absolute Protein Amount in Proteomics by the Number of Sequenced Peptides per Protein*S

  2005cited by this paper
• Toward a genome-wide systems biology analysis of host-pathogen interactions in group A Streptococcus.

  2005cited by this paper
• The global burden of group A streptococcal diseases.

  2005cited by this paper
• The structural biology of type II fatty acid biosynthesis.

  2005cited by this paper
• A uniform proteomics MS/MS analysis platform utilizing open XML file formats

  2005cited by this paper
• Open mass spectrometry search algorithm.

  2004cited by this paper
• Cytoscape: a software environment for integrated models of biomolecular interaction networks.

  2003cited by this paper
• A method for reducing the time required to match protein sequences with tandem mass spectra.

  2003cited by this paper
• A statistical model for identifying proteins by tandem mass spectrometry.

  2003cited by this paper
• Empirical statistical model to estimate the accuracy of peptide identifications made by MS/MS and database search.

  2002cited by this paper
• Proteolysis and its regulation at the surface of Streptococcus pyogenes

  2002cited by this paper
• The contact system.

  2002cited by this paper
• Complete genome sequence of an M1 strain of Streptococcus pyogenes

  2001cited by this paper
• Pathogenesis of group A streptococcal infections.

  2000cited by this paper
• Binding of RHOA G17V to p300 enhances its HAT activity: a new mechanism of epigenetic deregulation in TFH lymphoma

  1999cited by this paper
• Molecular analysis of the role of the group A streptococcal cysteine protease, hyaluronic acid capsule, and M protein in a murine model of human invasive soft-tissue infection.

  1998cited by this paper
• C repeats of the streptococcal M1 protein achieve the human serum albumin binding ability by flanking regions which stabilize the coiled-coil conformation.

  1997cited by this paper
• Protein PAB, an Albumin-binding Bacterial Surface Protein Promoting Growth and Virulence*

  1996cited by this paper
• Identification of interdomain sequences promoting the intronless evolution of a bacterial protein family.

  1996cited by this paper
• Biological properties of a Streptococcus pyogenes mutant generated by Tn916 insertion in mga.

  1995cited by this paper
• M1 protein and protein H: IgGFc- and albumin-binding streptococcal surface proteins encoded by adjacent genes.

  1994cited by this paper
• M12 protein from Streptococcus pyogenes is a receptor for immunoglobulin G3 and human albumin

  1994cited by this paper
• Protein PAB, a mosaic albumin-binding bacterial protein representing the first contemporary example of module shuffling.

  1994cited by this paper
• Molecular and biochemical analyses of fatty acid transport, metabolism, and gene regulation in Escherichia coli.

  1994cited by this paper
• Streptococcal M protein.

  1991cited by this paper
• A secreted receptor related to M1 protein of Streptococcus pyogenes binds to fibrinogen, IgG, and albumin.

  1990influential reference
• Streptococcal M protein: molecular design and biological behavior

  1989cited by this paper
• Streptococcal protein G, expressed by streptococci or by Escherichia coli, has separate binding sites for human albumin and IgG.

  1987cited by this paper
• Specific absorption of human serum albumin, immunoglobulin A, and immunoglobulin G with selected strains of group A and G streptococci

  1979cited by this paper
• A k-means clustering algorithm

  1979cited by this paper

• The Streptococcus pyogenes M protein is involved in phenotypic resistance to phage A25 infection in presence of human serum

  2024influential citation
• Quantification of Adaptive Immune Responses Against Protein-Binding Interfaces in the Streptococcal M1 Protein

  2024cites this paper
• Procoagulant Activity of Blood and Microvesicles Is Disturbed by Pneumococcal Pneumolysin, Which Interacts with Coagulation Factors

  2022cites this paper
• Proteomic Approaches to Unravel Mechanisms of Antibiotic Resistance and Immune Evasion of Bacterial Pathogens

  2022cites this paper
• Streptococcus pyogenes Forms Serotype- and Local Environment-Dependent Interspecies Protein Complexes

  2021cites this paper
• Protocol for Proteome Analysis of Group A Streptococcus.

  2020cites this paper
• Structural proteomics, electron cryo-microscopy and structural modeling approaches in bacteria–human protein interactions

  2020cites this paper
• Group A Streptococcus: Methods and Protocols

  2020influential citation
• Targeted Proteomics for Studying Pathogenic Bacteria

  2019cites this paper
• A quantitative Streptococcus pyogenes–human protein–protein interaction map reveals localization of opsonizing antibodies

  2019cites this paper
• Rapid determination of quaternary protein structures in complex biological samples

  2019cites this paper
• Proteomic analysis at the sites of clinical infection with invasive Streptococcus pyogenes

  2018cites this paper
• A holistic approach of host-pathogen interactions

  2018cites this paper
• Comprehensive Mass Spectrometric Survey of Streptococcus pyogenes Subcellular Proteomes.

  2018cites this paper
• Streptococcus pyogenes Infection and the Human Proteome with a Special Focus on the Immunoglobulin G-cleaving Enzyme IdeS*

  2018cites this paper
• Greedy motif-based approach to parsing large and diverge coiled-coil proteins into domains

  2017cites this paper
• A Proteomic Signature of Dormancy in the Actinobacterium Micrococcus luteus

  2017cites this paper
• SCM, the M Protein of Streptococcus canis Binds Immunoglobulin G

  2017cites this paper
• Surface and Exoproteomes of Gram-Positive Pathogens for Vaccine Discovery.

  2017cites this paper
• A holistic approach to host-pathogen interactions: Detecting the large to unravel the small

  2017cites this paper
• Analysis of Bacterial Surface Interactions with Mass Spectrometry-Based Proteomics.

  2017cites this paper
• Quantitative multiplexed proteomics of Taenia solium cysts obtained from the skeletal muscle and central nervous system of pigs

  2017cites this paper
• Targeted Proteomics and Absolute Protein Quantification for the Construction of a Stoichiometric Host-Pathogen Surface Density Model*

  2017cites this paper
• Listeria monocytogenes remodels the cell surface in the blood-stage.

  2016cites this paper
• Genomic Landscape of Intrahost Variation in Group A Streptococcus: Repeated and Abundant Mutational Inactivation of the fabT Gene Encoding a Regulator of Fatty Acid Synthesis

  2016cites this paper
• A tool for the automated, targeted analysis of data-independent acquisition MS-data: OpenSWATH

  2016cites this paper
• Quantitative proteogenomics of human pathogens using DIA-MS.

  2015cites this paper
• Development of Phage-Based Antibody Fragment Reagents for Affinity Enrichment of Bacterial Immunoglobulin G Binding Proteins.

  2015cites this paper
• DIANA - algorithmic improvements for analysis of data-independent acquisition MS data

  2015cites this paper
• A comprehensive analysis of the Streptococcus pyogenes and human plasma protein interaction network.

  2014cites this paper
• Targeted mass spectrometry analysis of neutrophil-derived proteins released during sepsis progression

  2014cites this paper
• A tool for the automated, targeted analysis of data-independent acquisition MS-data: OpenSWATH

  2014cites this paper
• Surface proteins of group G Streptococcus in different phases of growth: patterns of production and implications for the host-bacteria relationship.

  2014cites this paper
• To harm or not to harm? On the evolution and expression of virulence in group A streptococci.

  2014cites this paper
• OpenSWATH enables automated, targeted analysis of data-independent acquisition MS data

  2014cites this paper
• An automated pipeline for high-throughput label-free quantitative proteomics.

  2013cites this paper
• A Novel Role for Pro-Coagulant Microvesicles in the Early Host Defense against Streptococcus pyogenes

  2013cites this paper
• Automated Selected Reaction Monitoring Software for Accurate Label-Free Protein Quantification

  2012cites this paper
• The Dynamic Streptococcus Pyogenes Transcriptome in the Host Cell Environment and Contributions by Phage

  2012cites this paper
• Proteome-wide selected reaction monitoring assays for the human pathogen Streptococcus pyogenes

  2012cites this paper
• Automated workflow for large-scale selected reaction monitoring experiments.

  2012cites this paper
• openBIS: a flexible framework for managing and analyzing complex data in biology research

  2011cites this paper