miR‐145 inhibits isoproterenol‐induced cardiomyocyte hypertrophy by targeting the expression and localization of GATA6

Excessive βAR stimulation is an independent factor in inducing pathological cardiac hypertrophy. Here, we report miR‐145 regulates both expression and localization of GATA6, thereby protecting the heart against cardiomyocyte hypertrophy induced by isoproterenol (ISO). The protective activity of miR‐145 was associated with down‐regulation of ANF, BNP and β‐MHC expression, a decreased rate of protein synthesis, inhibited cardiomyocyte growth and the modulation of several signaling pathways including ERK1/2, JNK and Akt‐GSK3β. The anti‐hypertrophic effect was abrogated by exogenous over‐expression of transcription factor GATA6 which was further identified as a direct target of miR‐145. In addition, GSK3β antagonists, LiCl and TDZD8, restored the nuclear accumulation of GATA6, which was attenuated by miR‐145 Finally, we observed a dynamic pattern of miR‐145 expression in ISO‐treated NRCMs and in the hearts of TAC mice. Together, our results identify miR‐145 as an important regulator in cardiac hypertrophy.

• Publication year

  2013

• Venue

  FEBS Letters

• Publication date

  2013-06-19

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/j.febslet.2013.04.018PMID 23624080PMCID PMC4183134
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Replacement treatment with microRNA-143 and -145 induces synergistic inhibition of the growth of human bladder cancer cells by regulating PI3K/Akt and MAPK signaling pathways.

  2013cited by this paper
• MicroRNA-145 Protects Cardiomyocytes against Hydrogen Peroxide (H2O2)-Induced Apoptosis through Targeting the Mitochondria Apoptotic Pathway

  2012cited by this paper
• Ischemic Postconditioning Mediates Cardioprotection Via PI3K/GSK-3&bgr;/&bgr;-Catenin Signaling Pathway in Ischemic Rat Myocardium

  2012cited by this paper
• Negative regulation of miR-145 by C/EBP-β through the Akt pathway in cancer cells

  2012influential reference
• cAMP-dependent proteolysis of GATA-6 is linked to JNK-signaling pathway.

  2012influential reference
• MicroRNA-145 is regulated by DNA methylation and p53 gene mutation in prostate cancer.

  2011influential reference
• MiR-145 directly targets p70S6K1 in cancer cells to inhibit tumor growth and angiogenesis

  2011cited by this paper
• β-Adrenergic Receptor-PI3K Signaling Crosstalk in Mouse Heart: Elucidation of Immediate Downstream Signaling Cascades

  2011cited by this paper
• Krüppel-like factor 4 regulates pressure-induced cardiac hypertrophy.

  2010cited by this paper
• The Transcription Factor GATA-6 Regulates Pathological Cardiac Hypertrophy

  2010cited by this paper
• Unique MicroRNA Profile in End-stage Heart Failure Indicates Alterations in Specific Cardiovascular Signaling Networks*

  2009cited by this paper
• MicroRNAs and cardiac pathology

  2009cited by this paper
• Deletion of GSK-3beta in mice leads to hypertrophic cardiomyopathy secondary to cardiomyoblast hyperproliferation.

  2008cited by this paper
• Parathyroid hormone-related protein induces cell survival in human renal cell carcinoma through the PI3K Akt pathway: evidence for a critical role for integrin-linked kinase and nuclear factor kappa B.

  2007cited by this paper
• Cardiac hypertrophy induced by sustained β-adrenoreceptor activation: pathophysiological aspects

  2007cited by this paper
• Role of β-adrenergic receptor signaling and desensitization in heart failure: new concepts and prospects for treatment

  2006cited by this paper
• Characterization of cAMP-dependent proteolysis of GATA-6.

  2005cited by this paper
• The GATA family (vertebrates and invertebrates).

  2002cited by this paper
• Glycogen synthase kinase 3beta regulates GATA4 in cardiac myocytes.

  2001cited by this paper
• The Transcription Factors GATA4 and GATA6 Regulate Cardiomyocyte Hypertrophy in Vitro and in Vivo *

  2001cited by this paper
• The Zinc Finger-containing Transcription Factors GATA-4, -5, and -6

  2000cited by this paper
• Cooperative Interaction between GATA-4 and GATA-6 Regulates Myocardial Gene Expression

  1999cited by this paper

• The Heart’s Small Molecules: The Importance of MicroRNAs in Cardiovascular Health

  2025cites this paper
• miR-30a-5p preserves cardiac homeostasis by reprogramming metabolic checkpoints in hypertrophic cardiomyopathy.

  2025cites this paper
• The lncRNA Jpx participates in testosterone-induced H9c2 cell hypertrophy by targeting the miR-145-5p/Nfatc3 axis

  2025influential citation
• Plasma microRNA-143 and microRNA-145 levels are elevated in patients with left ventricular dysfunction

  2024cites this paper
• MicroRNA-133a and MicroRNA-145 May Be Involved in the Development of Hypertension-Mediated Organ Damage in Children with Primary Hypertension—A Preliminary Study

  2024cites this paper
• β stimulator induces upregulation of miR-27a in the rat heart one hour after the injection.

  2024cites this paper
• Tissue-specific transcriptional programming of macrophages controls the microRNA transcriptome targeting multiple functional pathways

  2024cites this paper
• An Increase in Plasma MicroRNA-143 Levels in the Acute Phase Is Positively Correlated With Recovery of Cardiac Function in the Chronic Phase in Patients With Acute Myocardial Infarction.

  2023cites this paper
• Clinical Significance of MicroRNAs, Long Non-Coding RNAs, and CircRNAs in Cardiovascular Diseases

  2023cites this paper
• A cellular regulator of the niche: telocyte

  2022cites this paper
• Effectual Endeavors of Silk Protein Sericin against Isoproterenol Induced Cardiac Toxicity and Hypertrophy in Wistar Rats

  2022cites this paper
• Relationship Between Circulating MicroRNAs and Left Ventricular Hypertrophy in Hypertensive Patients

  2022cites this paper
• Discriminating aspects of global metabolism of neonatal cardiomyocytes from wild type and KO-CSRP3 rats using proton magnetic resonance spectroscopy of culture media samples

  2020cites this paper
• Role of MicroRNA-145 in DNA Damage Signalling and Senescence in Vascular Smooth Muscle Cells of Type 2 Diabetic Patients

  2020cites this paper
• The role and molecular mechanism of epigenetics in cardiac hypertrophy

  2020cites this paper
• Non-coding RNAs in Cardiovascular Diseases

  2020cites this paper
• Non-coding RNAs in Physiological Cardiac Hypertrophy.

  2020cites this paper
• Non-coding RNAs: The key detectors and regulators in cardiovascular disease.

  2020cites this paper
• СИСТЕМНІ ВЗАЄМОДІЇ В МІКРОРНК У ПАТОГЕНЕЗІ СЕРЦЕВО-СУДИННИХ ЗАХВОРЮВАНЬ

  2019cites this paper
• Antihypertrophic Effects of Nebivolol on Neonatal Cardiomyocyte Hypertrophy Models

  2019cites this paper
• Marked disparity of microRNA modulation by cGMP-selective PDE5 versus PDE9 inhibitors in heart disease.

  2018cites this paper
• Geniposide Attenuates LPS-Induced Injury via Up-Regulation of miR-145 in H9c2 Cells

  2018cites this paper
• ClC-3 chloride channel is involved in isoprenaline-induced cardiac hypertrophy.

  2018cites this paper
• At the heart of programming: the role of micro-RNAs

  2018cites this paper
• Noncoding RNAs in Cardiac Hypertrophy

  2018cites this paper
• Emerging Role of mTOR Signaling-Related miRNAs in Cardiovascular Diseases

  2018cites this paper
• miRNA-145 is associated with spontaneous hypertension by targeting SLC7A1.

  2017cites this paper
• MicroRNA as a Therapeutic Target in Cardiac Remodeling

  2017cites this paper
• Overexpression of microRNA-145 protects against rat myocardial infarction through targeting PDCD4.

  2017cites this paper
• MiRNA Deregulation in Cardiac Aging and Associated Disorders.

  2017cites this paper
• Overview of MicroRNAs in Cardiac Hypertrophy, Fibrosis, and Apoptosis

  2016cites this paper
• The Signature of MicroRNA Dysregulation in Muscle Paralyzed by Spinal Cord Injury Includes Downregulation of MicroRNAs that Target Myostatin Signaling

  2016cites this paper
• Airway smooth muscle inflammation is regulated by microRNA‐145 in COPD

  2016cites this paper
• Construction and analysis of cardiac hypertrophy-associated lncRNA-mRNA network based on competitive endogenous RNA reveal functional lncRNAs in cardiac hypertrophy

  2016cites this paper
• Basic Science and Experimental Study Micro-RNA Expression in Hypoplastic Left Heart Syndrome

  2015cites this paper
• MicroRNA-143/-145 in Cardiovascular Diseases

  2015cites this paper
• Micro-RNA expression in hypoplastic left heart syndrome.

  2015cites this paper
• Sex- and oestrogen-dependent regulation of miRNAs in cardiac hypertrophy

  2015cites this paper
• Chlorogenic acid prevents isoproterenol-induced hypertrophy in neonatal rat myocytes.

  2014cites this paper
• The diverse roles of RNA polymerase II C-terminal domain phosphatase SCP1

  2014cites this paper
• Perfil de expressão de microRNAS no miocárdio na infecção aguda pelo Trypanosoma cruzi em camundongos

  2014cites this paper
• A sensitive and fast LC-MS/MS method for determination of β-receptor agonist JP-49b: application to a pharmacokinetic study in rats.

  2014cites this paper