Gentiobiose, a constituent of deoxyribonucleic acid from coliphage T6.

The deoxyribonucleic acid of the T-even coliphages contains glucosylated and nonglucosylated 5-hydroxymethylcytosine in place of cytosine (l-6). In the case of deoxyribonucleic acid from phage T6, 72y0 of the 5-hydroxymethylcytosine residues are diglucosylated. In T2 deoxyribonucleic acid, 70% of the 5-hydroxymethylcytosine residues are monoglucosylated, but 5% bear diglucosyl units. A recent investigation of the configuration of the disaccharides bound to the 5-hydroxymethylcytosine nucleotides of deoxyribonucleic acid from T2 and T6 showed the glucose units to be p linked to each other and joined to the hydroxymethyl group of the 5-hydroxymethylcytosine by an cr linkage (6). In the present study, the disaccharide from T6 deoxyribonucleic acid was identified as gentiobiose, 6-O-O-D-ghcOpyranOSyl-D-ghCOSe. This disaccharide is unique to deoxyribonucleic acid from phage T6 and possibly T2 and has been observed only in such glycosides as amygdalin and crocin, and as part of the trisaccharide, gentianose.

• Publication year

  1962

• Venue

  Journal of Biological Chemistry

• Publication date

  1962-04-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/s0021-9258(18)60319-9PMID 14460593
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• Gentiobiose is identified as a constituent disaccharide of phage T6 DNA, and it is described as possibly present in phage T2 DNA as well.

  Confidence 0.98

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewq (76h6bfydm6) review

• 5-hydroxymethylcytosine

  nucleobase

  A cytosine analog bearing a hydroxymethyl group at the 5 position.

  Aliases: 5-HMC

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewq (76h6bfydm6) review
• gentiobiose

  carbohydrate

  A disaccharide made of two glucose residues linked through a beta glycosidic bond.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewq (76h6bfydm6) review
• phage t2 dna

  nucleic acid

  The deoxyribonucleic acid extracted from bacteriophage T2.

  Aliases: T2 DNA, T2 deoxyribonucleic acid

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewq (76h6bfydm6) review
• phage t6 dna

  nucleic acid

  The deoxyribonucleic acid extracted from bacteriophage T6.

  Aliases: T6 DNA, T6 deoxyribonucleic acid

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) reviewq (76h6bfydm6) review

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