Transcriptome and hormone profiling reveals Eucalyptus grandis defence responses against Chrysoporthe austroafricana

BackgroundEucalyptus species and interspecific hybrids exhibit valuable growth and wood properties that make them a highly desirable commodity. However, these trees are challenged by a wide array of biotic stresses during their lifetimes. The Eucalyptus grandis reference genome sequence provides a resource to study pest and pathogen defence mechanisms in long-lived woody plants. E. grandis trees are generally susceptible to Chrysoporthe austroafricana, a causal agent of stem cankers on eucalypts. The aim of this study was to characterize the defence response of E. grandis against C. austroafricana.ResultsHormone profiling of susceptible and moderately resistant clonal E. grandis genotypes indicated a reduction in salicylic acid and gibberellic acid levels at 3 days post inoculation. We hypothesized that these signaling pathways may facilitate resistance. To further investigate other defence mechanisms at this time point, transcriptome profiling was performed. This revealed that cell wall modifications and response to oxidative stress form part of the defence responses common to both genotypes, whilst changes in the hormone signaling pathways may contribute to resistance. Additionally the expression of selected candidate defence response genes was induced earlier in moderately resistant trees than in susceptible trees, supporting the hypothesis that a delayed defence response may occur in the susceptible interaction.ConclusionThe ability of a host to fine-tune its defence responses is crucial and the responses identified in this study extends our understanding of plant defence, gained from model systems, to woody perennials.

• Publication year

  2015

• Venue

  BMC Genomics

• Publication date

  2015-04-18

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1186/s12864-015-1529-xPMID 25903559PMCID 4405875
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Tree immunity: growing old without antibodies.

  2014cited by this paper
• Signaling by small metabolites in systemic acquired resistance.

  2014cited by this paper
• The genome of Eucalyptus grandis

  2014cited by this paper
• Connecting growth and defense: the emerging roles of brassinosteroids and gibberellins in plant innate immunity.

  2014cited by this paper
• Uncovering the defence responses of Eucalyptus to pests and pathogens in the genomics age.

  2014cited by this paper
• Long-distance communication and signal amplification in systemic acquired resistance

  2013cited by this paper
• The ethylene receptor ETR1 is required for Fusarium oxysporum pathogenicity

  2013cited by this paper
• Calcium-Dependent Protein Kinases: Hubs in Plant Stress Signaling and Development1

  2013cited by this paper
• The identification and differential expression of Eucalyptus grandis pathogenesis-related genes in response to salicylic acid and methyl jasmonate

  2013cited by this paper
• Established and new technologies reduce increasing pest and pathogen threats to Eucalypt plantations

  2013cited by this paper
• MAPK cascades in plant disease resistance signaling.

  2013cited by this paper
• Susceptibility to plant disease: more than a failure of host immunity.

  2013cited by this paper
• Reactive oxygen species and their role in plant defence and cell wall metabolism

  2012cited by this paper
• Gibberellin Signaling: A Theme and Variations on DELLA Repression1

  2012cited by this paper
• Plant immunity to necrotrophs.

  2012cited by this paper
• Disruption of sphingolipid biosynthesis in Nicotiana benthamiana activates salicylic acid-dependent responses and compromises resistance to Alternaria alternata f. sp. lycopersici

  2012cited by this paper
• Hormonal modulation of plant immunity.

  2012cited by this paper
• Paradigms and paradox in the ethylene signaling pathway and interaction network.

  2011cited by this paper
• Respiratory burst oxidases: the engines of ROS signaling.

  2011cited by this paper
• The molecular mechanism and evolution of the GA-GID1-DELLA signaling module in plants.

  2011cited by this paper
• SCARECROW-LIKE 3 promotes gibberellin signaling by antagonizing master growth repressor DELLA in Arabidopsis

  2011cited by this paper
• Hormone crosstalk in plant disease and defense: more than just jasmonate-salicylate antagonism.

  2011cited by this paper
• Pectin methylesterase is induced in Arabidopsis upon infection and is necessary for a successful colonization by necrotrophic pathogens.

  2011cited by this paper
• Eucalypts as a biofuel feedstock

  2011cited by this paper
• PIN polarity maintenance by the cell wall in Arabidopsis.

  2011cited by this paper
• Galaxy: a comprehensive approach for supporting accessible, reproducible, and transparent computational research in the life sciences

  2010cited by this paper
• Functional analyses of differentially expressed isoforms of the Arabidopsis inositol phosphorylceramide synthase

  2010cited by this paper
• Galaxy: A Web‐Based Genome Analysis Tool for Experimentalists

  2010cited by this paper
• Future Perspectives in Plant Biology Plant Immunity : It ’ s the Hormones Talking , But What Do They Say ?

  2010cited by this paper
• Plant Immunity: It’s the Hormones Talking, But What Do They Say?

  2010cited by this paper
• Fungal phytotoxins as mediators of virulence.

  2009cited by this paper
• Development of a method for comprehensive and quantitative analysis of plant hormones by highly sensitive nanoflow liquid chromatography-electrospray ionization-ion trap mass spectrometry.

  2009cited by this paper
• Use of a Synthetic Salicylic Acid Analog to Investigate the Roles of Methyl Salicylate and Its Esterases in Plant Disease Resistance*

  2009cited by this paper
• Kinetics of Salicylate-Mediated Suppression of Jasmonate Signaling Reveal a Role for Redox Modulation1[OA]

  2008cited by this paper
• An Inositolphosphorylceramide Synthase Is Involved in Regulation of Plant Programmed Cell Death Associated with Defense in Arabidopsis[C][W]

  2008cited by this paper
• DELLAs control plant immune responses by modulating the balance of jasmonic acid and salicylic acid signaling.

  2008cited by this paper
• Pathological hormone imbalances.

  2007cited by this paper
• Methyl Salicylate Is a Critical Mobile Signal for Plant Systemic Acquired Resistance

  2007cited by this paper
• qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data

  2007cited by this paper
• Galaxy: a platform for interactive large-scale genome analysis.

  2005cited by this paper
• Relative Pathogenicity of Cryphonectria cubensis on Eucalyptus Clones Differing in Their Resistance to C. cubensis.

  2005cited by this paper
• Spatial and temporal analysis of the local response to wounding in Arabidopsis leaves.

  2004cited by this paper
• MAPMAN: a user-driven tool to display genomics data sets onto diagrams of metabolic pathways and other biological processes.

  2004cited by this paper
• Chrysoporthe, a new genus to accommodate Cryphonectria cubensis

  2004cited by this paper
• Biological and Phylogenetic Analyses Suggest that Two Cryphonectria spp. Cause Cankers of Eucalyptus in Africa.

  2003cited by this paper
• Susceptible to intolerance--a range of hormonal actions in a susceptible Arabidopsis pathogen response.

  2003cited by this paper
• RNA isolation from highly viscous samples rich in polyphenols and polysaccharides

  2002cited by this paper
• The GRAS gene family in Arabidopsis: sequence characterization and basic expression analysis of the SCARECROW-LIKE genes.

  1999cited by this paper
• The Arabidopsis RGA Gene Encodes a Transcriptional Regulator Repressing the Gibberellin Signal Transduction Pathway

  1998cited by this paper
• Bmc Plant Biology Co-expression and Promoter Content Analyses Assign a Role in Biotic and Abiotic Stress Responses to Plant Natriuretic Peptides

  year unknowncited by this paper

• A weighted gene co-expression network analysis characterises the common defence responses of Eucalyptus to diverse biotic challenges

  2026cites this paper
• A haplotype-resolved reference genome for Eucalyptus grandis

  2025cites this paper
• Enhancing Plantation Forest Sustainability: A Review of Eucalyptus Defence Mechanisms to Foliar Fungal Pathogens

  2025cites this paper
• Pathogenicity of Chrysoporthe deuterocubensis on eucalypts in Indonesia

  2024cites this paper
• Molecular mechanisms underlying tree host-pathogen interactions under drought stress and subsequent rewatering in Eucalyptus grandis

  2024cites this paper
• Age trends of genetic parameters and genotype-by-environment interactions for growth traits of Eucalyptus urophylla clones in South-China

  2024cites this paper
• Innovations in Antifungal Drug Discovery among Cell Envelope Synthesis Enzymes through Structural Insights

  2024cites this paper
• Transcriptional reprogramming during recovery from drought stress in Eucalyptus grandis.

  2023cites this paper
• Mechanisms of systemic resistance to pathogen infection in plants and their potential application in forestry

  2023cites this paper
• Genome-wide identification and expression profile analysis of metal tolerance protein gene family in Eucalyptus grandis under metal stresses

  2023cites this paper
• Genomic consequences of artificial selection during early domestication of a wood fibre crop

  2022cites this paper
• Transcriptome profiling of Toona ciliata young stems in response to Hypsipyla robusta Moore

  2022cites this paper
• qtlXplorer: an online systems genetics browser in the Eucalyptus Genome Integrative Explorer (EucGenIE)

  2021cites this paper
• Dual RNA-Sequencing Analysis of Resistant (Pinus pinea) and Susceptible (Pinus radiata) Hosts during Fusarium circinatum Challenge

  2021cites this paper
• eCALIBRATOR: A Comparative Tool to Identify Key Genes and Pathways for Eucalyptus Defense Against Biotic Stressors

  2020influential citation
• Does disease severity impact on plant foliar chemical and physical responses to two Corymbia citriodora subsp. variegata pathogens?

  2020cites this paper
• Genomic evidence of introgression and adaptation in a model subtropical tree species, Eucalyptus grandis

  2020cites this paper
• Insect egg-induced physiological changes and transcriptional reprogramming leading to gall formation.

  2020cites this paper
• Transcriptome analysis identifies genes involved in the somatic embryogenesis of Eucalyptus

  2020cites this paper
• The Threat of the Combined Effect of Biotic and Abiotic Stress Factors in Forestry Under a Changing Climate

  2020cites this paper
• The interplay between microRNA and alternative splicing of linear and circular RNAs in eleven plant species

  2019cites this paper
• Pinus Susceptibility to Pitch Canker Triggers Specific Physiological Responses in Symptomatic Plants: An Integrated Approach

  2019cites this paper
• Transcriptional response of grapevine to infection with the fungal pathogen Lasiodiplodia theobromae

  2019cites this paper
• The Road to Resistance in Forest Trees

  2019cites this paper
• Active Fungal Communities in Asymptomatic Eucalyptus grandis Stems Differ between a Susceptible and Resistant Clone

  2019influential citation
• The identification of small molecule inhibitors of the plant inositol phosphorylceramide synthase which demonstrate herbicidal activity

  2019cites this paper
• Hardwood Tree Genomics: Unlocking Woody Plant Biology

  2018cites this paper
• Genome-wide annotation and expression responses to biotic stresses of the WALL-ASSOCIATED KINASE - RECEPTOR-LIKE KINASE (WAK-RLK) gene family in Apple (Malus domestica)

  2018cites this paper
• Plant–pathogen interactions: leaf physiology alterations in poplars infected with rust (Melampsora medusae)

  2018cites this paper
• Evidence for salicylic acid signalling and histological changes in the defence response of Eucalyptus grandis to Chrysoporthe austroafricana

  2017cites this paper
• De Novo Transcriptome Study Identifies Candidate Genes Involved in Resistance to Austropuccinia psidii (Myrtle Rust) in Syzygium luehmannii (Riberry).

  2017cites this paper
• Identification of the Eucalyptus grandis chitinase gene family and expression characterization under different biotic stress challenges

  2017cites this paper
• Neofusicoccum parvum Colonization of the Grapevine Woody Stem Triggers Asynchronous Host Responses at the Site of Infection and in the Leaves

  2017cites this paper
• Transcriptome profiling of Eucalyptus nitens reveals deeper insight into the molecular mechanism of cold acclimation and deacclimation process

  2017cites this paper
• RNA-Seq indica que Polietilenoglicol induz estresse de hipóxia nas raízes de Eucalyptus em hidroponia

  2017cites this paper
• Molecular analysis of the interaction between white rot pathogen (Rigidoporus microporus) and rubber tree (Hevea brasiliensis)

  2016cites this paper
• The Eucalyptus grandis NBS-LRR Gene Family: Physical Clustering and Expression Hotspots

  2016cites this paper
• Localization and Transcriptional Responses of Chrysoporthe austroafricana in Eucalyptus grandis Identify Putative Pathogenicity Factors

  2016influential citation
• Defence‐related gene expression of Hevea brasiliensis clones in response to the white rot pathogen, Rigidoporus microporus

  2016cites this paper