{"corpus_id":10205083,"paper_sha":"4d256b201b2f63e150531f76100def15ef937dd4","doi":"10.1016/s0021-9258(19)50281-2","arxiv_id":null,"pmid":8099356,"pmcid":null,"mag_id":1585661831,"dblp_id":null,"acl_id":null,"title":"Sequence and structural implications of a bovine corneal keratan sulfate proteoglycan core protein. Protein 37B represents bovine lumican and proteins 37A and 25 are unique.","year":1993,"publication_date":"1993-06-05","venue":"Journal of Biological Chemistry","journal":{"name":"The Journal of biological chemistry","pages":"\n          11874-80\n        ","volume":"268 16"},"journal_issn":null,"journal_title":null,"publication_types":["JournalArticle","Study"],"pubmed_pub_types":["Comparative Study","Journal Article","Research Support, Non-U.S. Gov't","Research Support, U.S. Gov't, Non-P.H.S.","Research Support, U.S. Gov't, P.H.S."],"s2_fields_of_study":["Biology","Medicine"],"reference_count":0,"citation_count":106,"influential_citation_count":0,"is_open_access":false,"arxiv_categories":null,"arxiv_license":null,"arxiv_journal_ref":null,"mesh_headings":[{"d":"Amino Acid Sequence","mj":false,"ui":"D000595"},{"d":"Animals","mj":false,"ui":"D000818"},{"d":"Base Sequence","mj":false,"ui":"D001483"},{"d":"Cattle","mj":false,"ui":"D002417"},{"d":"Cells, Cultured","mj":false,"ui":"D002478"},{"d":"Chickens","mj":false,"ui":"D002645"},{"d":"Chondroitin Sulfate Proteoglycans","mj":false,"qs":[{"q":"chemistry","mj":false,"ui":"Q000737"},{"q":"genetics","mj":true,"ui":"Q000235"}],"ui":"D011508"},{"d":"Cornea","mj":false,"qs":[{"q":"metabolism","mj":true,"ui":"Q000378"}],"ui":"D003315"},{"d":"DNA","mj":false,"qs":[{"q":"genetics","mj":false,"ui":"Q000235"},{"q":"isolation & purification","mj":false,"ui":"Q000302"}],"ui":"D004247"},{"d":"Gene Library","mj":false,"ui":"D015723"},{"d":"Keratan Sulfate","mj":false,"qs":[{"q":"chemistry","mj":false,"ui":"Q000737"},{"q":"genetics","mj":true,"ui":"Q000235"}],"ui":"D007632"},{"d":"Lumican","mj":false,"ui":"D000071234"},{"d":"Male","mj":false,"ui":"D008297"},{"d":"Molecular Sequence Data","mj":false,"ui":"D008969"},{"d":"Mutagenesis","mj":false,"ui":"D016296"},{"d":"Orchiectomy","mj":false,"ui":"D009919"},{"d":"Peptide Fragments","mj":false,"qs":[{"q":"isolation & purification","mj":false,"ui":"Q000302"}],"ui":"D010446"},{"d":"Plasmids","mj":false,"ui":"D010957"},{"d":"Poly A","mj":false,"qs":[{"q":"genetics","mj":false,"ui":"Q000235"},{"q":"isolation & purification","mj":false,"ui":"Q000302"}],"ui":"D011061"},{"d":"Protein Structure, Secondary","mj":false,"ui":"D017433"},{"d":"RNA, Messenger","mj":false,"qs":[{"q":"genetics","mj":false,"ui":"Q000235"},{"q":"isolation & purification","mj":false,"ui":"Q000302"}],"ui":"D012333"},{"d":"Repetitive Sequences, Nucleic Acid","mj":false,"ui":"D012091"},{"d":"Restriction Mapping","mj":false,"ui":"D015183"},{"d":"Sequence Deletion","mj":false,"ui":"D017384"},{"d":"Sequence Homology, Amino Acid","mj":false,"ui":"D017386"},{"d":"Trypsin","mj":false,"ui":"D014357"}],"chemicals":[{"n":"Chondroitin Sulfate Proteoglycans","ui":"D011508","reg":"0"},{"n":"Lumican","ui":"D000071234","reg":"0"},{"n":"Peptide Fragments","ui":"D010446","reg":"0"},{"n":"RNA, Messenger","ui":"D012333","reg":"0"},{"n":"Poly A","ui":"D011061","reg":"24937-83-5"},{"n":"DNA","ui":"D004247","reg":"9007-49-2"},{"n":"Keratan Sulfate","ui":"D007632","reg":"9056-36-4"},{"n":"Trypsin","ui":"D014357","reg":"EC 3.4.21.4"}],"comments_corrections":null,"source_flags":5,"s2_open_access_pdf_url":null,"s2_open_access_landing_url":null,"s2_open_access_license":null,"s2_open_access_status":null,"pmc_open_access_pdf_url":null,"pmc_open_access_landing_url":null,"pmc_open_access_license":null,"pmc_open_access_status":null,"unpaywall_open_access_pdf_url":null,"unpaywall_open_access_landing_url":null,"unpaywall_open_access_license":null,"unpaywall_open_access_status":null,"abstract":"Amino acid sequence from tryptic peptides of three different bovine corneal keratan sulfate proteoglycan (KSPG) core proteins (designated 37A, 37B, and 25) showed similarities to the sequence of a chicken KSPG core protein lumican. Bovine lumican cDNA was isolated from a bovine corneal expression library by screening with chicken lumican cDNA. The bovine cDNA codes for a 342-amino acid protein, M(r) 38,712, containing amino acid sequences identified in the 37B KSPG core protein. The bovine lumican is 68% identical to chicken lumican, with an 83% identity excluding the N-terminal 40 amino acids. Location of 6 cysteine and 4 consensus N-glycosylation sites in the bovine sequence were identical to those in chicken lumican. Bovine lumican had about 50% identity to bovine fibromodulin and 20% identity to bovine decorin and biglycan. About two-thirds of the lumican protein consists of a series of 10 amino acid leucine-rich repeats that occur in regions of calculated high beta-hydrophobic moment, suggesting that the leucine-rich repeats contribute to beta-sheet formation in these proteins. Sequences obtained from 37A and 25 core proteins were absent in bovine lumican, thus predicting a unique primary structure and separate mRNA for each of the three bovine KSPG core proteins.","claims":[{"public_id":"cl_432c472123cd3c2c01489b95d0cad94e","status":"active","text":"Bovine lumican cDNA encodes a 342-amino acid protein of molecular mass 38,712 Da that contains the peptide sequences identified in protein 37B.","confidence":0.97,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_432c472123cd3c2c01489b95d0cad94e"},{"public_id":"cl_6838ced42cfad6b41afbe140e1ed19bd","status":"active","text":"Bovine lumican is 68% identical to chicken lumican overall and 83% identical when the N-terminal 40 amino acids are excluded, with conserved cysteine positions and N-glycosylation sites.","confidence":0.96,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_6838ced42cfad6b41afbe140e1ed19bd"},{"public_id":"cl_e5409a98350a3448ab1c003a5fdcbb9e","status":"active","text":"Leucine-rich repeats make up about two-thirds of bovine lumican and occur in regions of high beta-hydrophobic moment, suggesting a role in beta-sheet formation.","confidence":0.9,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_e5409a98350a3448ab1c003a5fdcbb9e"},{"public_id":"cl_b48b455247901ae497bc355c9471f620","status":"active","text":"Protein 37A and protein 25 contain sequences absent from bovine lumican, implying unique primary structures and separate mRNAs for all three bovine corneal keratan sulfate proteoglycan core proteins.","confidence":0.95,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_b48b455247901ae497bc355c9471f620"},{"public_id":"cl_7cd3362d0a3203ae9a16d2b80d74fac6","status":"active","text":"Protein 37B corresponds to bovine lumican, because its peptide sequences match sequences present in bovine lumican cDNA and are similar to chicken lumican.","confidence":0.98,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_7cd3362d0a3203ae9a16d2b80d74fac6"}],"concepts":[{"public_id":"co_160839a0c2cac84f613b6b201333d6a6","status":"active","name":"bovine lumican cDNA","description":"A complementary DNA clone isolated from a bovine corneal expression library using chicken lumican cDNA as a 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study.","types":["protein"],"aliases":["25"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_3f90503acd886a45904b952544cf18c9"},{"public_id":"co_40c00df4c0739d109f32d0b42c3f1170","status":"active","name":"N-glycosylation sites","description":"Consensus amino acid positions where N-linked glycosylation can occur in the lumican sequence.","types":["sequence feature"],"aliases":["consensus N-glycosylation sites"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_40c00df4c0739d109f32d0b42c3f1170"},{"public_id":"co_ae0dc73cca73f717d21a4da0ebe4dc3f","status":"active","name":"chicken lumican","description":"The lumican protein from chicken used for sequence comparison and cDNA 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