{"corpus_id":103902882,"paper_sha":"efdde18c5603c21461bdeb3de1a06ff8217c3853","doi":"10.1007/s00216-020-03058-w","arxiv_id":null,"pmid":33241445,"pmcid":"7813709","mag_id":2770832980,"dblp_id":null,"acl_id":null,"title":"Fluorescence polarization immunoassay for the determination of diclofenac in wastewater","year":2020,"publication_date":"2020-11-25","venue":"Analytical and Bioanalytical Chemistry","journal":{"name":"Analytical and Bioanalytical Chemistry","pages":"999 - 1007","volume":"413"},"journal_issn":null,"journal_title":null,"publication_types":["JournalArticle"],"pubmed_pub_types":["Journal Article"],"s2_fields_of_study":["Medicine","Chemistry","Environmental Science"],"reference_count":34,"citation_count":26,"influential_citation_count":0,"is_open_access":true,"arxiv_categories":null,"arxiv_license":null,"arxiv_journal_ref":null,"mesh_headings":[{"d":"Anti-Inflammatory Agents, Non-Steroidal","mj":false,"qs":[{"q":"analysis","mj":true,"ui":"Q000032"}],"ui":"D000894"},{"d":"Diclofenac","mj":false,"qs":[{"q":"analysis","mj":true,"ui":"Q000032"}],"ui":"D004008"},{"d":"Fluorescence Polarization Immunoassay","mj":false,"qs":[{"q":"methods","mj":true,"ui":"Q000379"}],"ui":"D016231"},{"d":"Limit of Detection","mj":false,"ui":"D057230"},{"d":"Wastewater","mj":false,"qs":[{"q":"analysis","mj":true,"ui":"Q000032"}],"ui":"D062065"},{"d":"Water Pollutants, Chemical","mj":false,"qs":[{"q":"analysis","mj":true,"ui":"Q000032"}],"ui":"D014874"}],"chemicals":[{"n":"Anti-Inflammatory Agents, Non-Steroidal","ui":"D000894","reg":"0"},{"n":"Diclofenac","ui":"D004008","reg":"144O8QL0L1"},{"n":"Wastewater","ui":"D062065","reg":"0"},{"n":"Water Pollutants, Chemical","ui":"D014874","reg":"0"}],"comments_corrections":null,"source_flags":5,"s2_open_access_pdf_url":"https://link.springer.com/content/pdf/10.1007/s00216-020-03058-w.pdf","s2_open_access_landing_url":"https://www.semanticscholar.org/paper/efdde18c5603c21461bdeb3de1a06ff8217c3853","s2_open_access_license":"CCBY","s2_open_access_status":"HYBRID","pmc_open_access_pdf_url":null,"pmc_open_access_landing_url":null,"pmc_open_access_license":null,"pmc_open_access_status":null,"unpaywall_open_access_pdf_url":null,"unpaywall_open_access_landing_url":null,"unpaywall_open_access_license":null,"unpaywall_open_access_status":null,"abstract":"Pharmacologically active compounds are often detected in wastewater and surface waters. The nonsteroidal anti-inflammatory drug diclofenac (DCF) was included in the European watch list of substances that requires its environmental monitoring in the member states. DCF may harmfully influence the ecosystem already at concentrations ≤ 1 μg L−1. The fast and easy quantification of DCF is becoming a subject of global importance. Fluorescence polarization immunoassay (FPIA) is a homogeneous mix-and-read method which does not require the immobilization of reagents. FPIA can be performed in one phase within 20–30 min, making it possible to analyse wastewater without any complicated pre-treatment. In this study, new tracer molecules with different structures, linking fluorophores to derivatives of the analyte, were synthesized, three homologous tracers based on DCF, two including a C6 spacer, and one heterologous tracer derived from 5-hydroxy-DCF. The tracer molecules were thoroughly assessed for performance. Regarding sensitivity of the FPIA, the lowest limit of detection reached was 2.0 μg L−1 with a working range up to 870 μg L−1. The method was validated for real wastewater samples against LC-MS/MS as reference method with good agreement of both methods. Graphical abstract Graphical abstract","claims":[{"public_id":"cl_769b6ac83976e7c48cb5bdfb6d01bb18","status":"active","text":"Fluorescence polarization immunoassay (FPIA) can quantify diclofenac in wastewater without complicated pre-treatment, with a limit of detection of 2.0 μg L⁻¹ and a working range up to 870 μg L⁻¹.","confidence":0.95,"contributors":[{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["extraction"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["review"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_769b6ac83976e7c48cb5bdfb6d01bb18"},{"public_id":"cl_3e99cde940da53aae1b2445bd1d0484a","status":"active","text":"The FPIA method was validated for real wastewater samples against LC-MS/MS as reference method with good agreement.","confidence":0.9,"contributors":[{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["extraction"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["review"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_3e99cde940da53aae1b2445bd1d0484a"},{"public_id":"cl_3c3a3019f03b0187caf22c6e7a2ae647","status":"active","text":"Three homologous tracers based on diclofenac and one heterologous tracer derived from 5-hydroxy-diclofenac were synthesized and assessed for performance in the FPIA.","confidence":0.9,"contributors":[{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["extraction"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["review"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_3c3a3019f03b0187caf22c6e7a2ae647"}],"concepts":[{"public_id":"co_01d259712a4aa8d739b4018be8b1fb82","status":"active","name":"5-hydroxy-diclofenac","description":"A derivative of diclofenac used to synthesize the heterologous tracer.","types":["chemical derivative"],"aliases":[],"contributors":[{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["extraction"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["review"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_01d259712a4aa8d739b4018be8b1fb82"},{"public_id":"co_1e70622dd5805a76b489e19fc726fc82","status":"active","name":"wastewater","description":"Aqueous waste matrix in which diclofenac is detected and quantified without complicated pre-treatment.","types":["sample matrix"],"aliases":[],"contributors":[{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["extraction"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["review"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_1e70622dd5805a76b489e19fc726fc82"},{"public_id":"co_60b32bce2da6102e7f1c405d2a640630","status":"active","name":"diclofenac","description":"A nonsteroidal anti-inflammatory drug (NSAID) that is monitored in wastewater and surface waters due to potential ecological harm.","types":["analyte"],"aliases":["DCF"],"contributors":[{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["extraction"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["review"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_60b32bce2da6102e7f1c405d2a640630"},{"public_id":"co_6f3223ad598b01917644e88e371b98e5","status":"active","name":"heterologous tracer","description":"A tracer molecule derived from 5-hydroxy-diclofenac, differing from the analyte structure, used in the FPIA.","types":["reagent"],"aliases":[],"contributors":[{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["extraction"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["review"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_6f3223ad598b01917644e88e371b98e5"},{"public_id":"co_71e81175e56e283170540badfd3b4c44","status":"active","name":"working range","description":"The concentration range over which the FPIA is effective, reported up to 870 μg L⁻¹.","types":["performance metric"],"aliases":[],"contributors":[{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["extraction"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["review"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_71e81175e56e283170540badfd3b4c44"},{"public_id":"co_7ffe761a1398001486b0e199e8c9897c","status":"active","name":"limit of detection","description":"The lowest concentration of diclofenac detectable by the FPIA, reported as 2.0 μg L⁻¹.","types":["performance metric"],"aliases":["LOD"],"contributors":[{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["extraction"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["review"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_7ffe761a1398001486b0e199e8c9897c"},{"public_id":"co_85c1ff3b5bb677258a82741cd6bea7a3","status":"active","name":"homologous tracers","description":"Tracer molecules based on diclofenac structure, including two with a C6 spacer, used in the FPIA.","types":["reagent"],"aliases":[],"contributors":[{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["extraction"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["review"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_85c1ff3b5bb677258a82741cd6bea7a3"},{"public_id":"co_9be201a189508f84ddac7e6ed654cf01","status":"active","name":"LC-MS/MS","description":"Liquid chromatography-tandem mass spectrometry used as the reference method for validation of the FPIA.","types":["reference method"],"aliases":[],"contributors":[{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["extraction"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["review"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_9be201a189508f84ddac7e6ed654cf01"},{"public_id":"co_ac353d5af245d62bc2179485f95439ba","status":"active","name":"fluorescence polarization immunoassay","description":"A homogeneous mix-and-read method that does not require immobilization of reagents, used here to quantify diclofenac in wastewater.","types":["method"],"aliases":["FPIA"],"contributors":[{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["extraction"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["review"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_ac353d5af245d62bc2179485f95439ba"},{"public_id":"co_eae05c6044c9f58c67330305169ea297","status":"active","name":"FPIA method","description":"The fluorescence polarization immunoassay procedure developed and validated for diclofenac quantification in wastewater.","types":["method"],"aliases":[],"contributors":[{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["extraction"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["review"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_eae05c6044c9f58c67330305169ea297"}],"external_ids":{"DOI":"10.1007/s00216-020-03058-w","ArXiv":null,"PubMed":33241445,"PubMedCentral":"7813709","MAG":2770832980,"DBLP":null,"ACL":null},"open_access":{"is_open_access":true,"pdf_url":"https://link.springer.com/content/pdf/10.1007/s00216-020-03058-w.pdf","landing_url":"https://www.semanticscholar.org/paper/efdde18c5603c21461bdeb3de1a06ff8217c3853","source":"semantic_scholar","pdf_url_source":"semantic_scholar_open_access_pdf","license":"CCBY","status":"HYBRID","reason":null},"reference_availability":{"status":"available","references_indexed":true,"full_text_available":false,"full_text_source":null,"count_basis":"semantic_scholar_metadata","extraction_status":"not_applicable","reason":null},"source":{"provider":"episteme2","base_corpus":"semantic_scholar_dump","freshness_mode":"unknown","basis":["semantic_scholar_metadata","postgres_metadata"],"limits":["paper metadata is based on indexed upstream scholarly datasets","claims and concepts are available only for extracted papers","absence of claims or concepts means no extracted graph data is available in this response"],"status":"available","degraded":false,"degraded_reasons":[],"diagnostics":{"status":"available","degraded":false,"degraded_reasons":[],"metadata_status":"available","graph_status":"available","abstract_status":"available"},"source_flags":5},"paper_id":632486,"paper_uid":"93124b2f-fa25-4670-b1ea-1fe116ffa9f5","canonical_identity":{"paper_id":632486,"paper_uid":"93124b2f-fa25-4670-b1ea-1fe116ffa9f5","identity_status":"available","lookup_basis":"semantic_scholar_external_id","compatibility_path":"corpus_id"},"url":"https://sah.borca.ai/papers/103902882"}