{"corpus_id":104466220,"paper_sha":"0ef0a1e5d8ee7d0a58a866033e7f7055d9dd7df6","doi":"10.1007/s10973-019-08151-6","arxiv_id":null,"pmid":null,"pmcid":null,"mag_id":2920417293,"dblp_id":null,"acl_id":null,"title":"Investigation of protein content of synovial fluids with DSC in different arthritides","year":2019,"publication_date":"2019-03-05","venue":"Journal of Thermal Analysis and Calorimetry","journal":{"name":"Journal of Thermal Analysis and Calorimetry","pages":"1-7","volume":""},"journal_issn":null,"journal_title":null,"publication_types":[],"pubmed_pub_types":null,"s2_fields_of_study":["Medicine","Chemistry"],"reference_count":57,"citation_count":14,"influential_citation_count":0,"is_open_access":true,"arxiv_categories":null,"arxiv_license":null,"arxiv_journal_ref":null,"mesh_headings":null,"chemicals":null,"comments_corrections":null,"source_flags":1,"s2_open_access_pdf_url":"https://link.springer.com/content/pdf/10.1007/s10973-019-08151-6.pdf","s2_open_access_landing_url":"https://www.semanticscholar.org/paper/0ef0a1e5d8ee7d0a58a866033e7f7055d9dd7df6","s2_open_access_license":"CCBY","s2_open_access_status":"HYBRID","pmc_open_access_pdf_url":null,"pmc_open_access_landing_url":null,"pmc_open_access_license":null,"pmc_open_access_status":null,"unpaywall_open_access_pdf_url":null,"unpaywall_open_access_landing_url":null,"unpaywall_open_access_license":null,"unpaywall_open_access_status":null,"abstract":"In the case of joint diseases, as a result of the process induced by the pathophysiological factors, the small articular structures that have pilled result in a qualitative and quantitative modification of the composition of the synovial fluid (SF). The analysis of this fluid offers a unique opportunity to study the joint diseases. In our study, 28 cases of acute knee joint meniscus injuries, transient knee joint synovitis, or degenerative knee joint destruction were studied. Following the collection and storage of SF, removed on the basis of the sample collection protocol, they were denatured in a SETARAM Micro DSC-II calorimeter with a scanning rate 0.3 K min−1 in the 20–90 °C temperature range. 4–4 samples from the “normal” SF batch were inoculated by two different strains of bacteria [Staphylococcus aureus and Staphylococcus epidermidis] with 10−3 colony-forming unit. Thermoanalytical changes of the proteins found in the SF were studied by a SETARAM Micro DSC-II, in the laboratory of the Biophysical Institute. The maximum denaturation temperature of the proteins being in the SF with different severity varied according to the criterion of the radiological scaling of osteoarthritis made by the Kellgren–Lawrence criterion. The decomposition of the scans showed the degree of the damage in case of SF as well as in the inoculated samples. Knowing the denaturing properties of temperature-dependent protein molecules in SF, DSC can be used to design and develop intra-articular lubricant additives that effectively enhance protein stability and contribute to a reduction in joint wear.","claims":[{"public_id":"cl_198bf72b675f379eeb7baf18cf72acf1","status":"active","text":"Decomposition of DSC thermograms revealed the degree of protein damage in synovial fluid from arthritis cases and in normal synovial fluid samples inoculated with Staphylococcus aureus and Staphylococcus epidermidis.","confidence":0.85,"contributors":[{"id":171,"public_id":"b9tnx83g25","public_label":"eunsjani (b9tnx83g25)","roles":["extraction"],"url":"https://sah.borca.ai/u/b9tnx83g25"},{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["review"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":17,"public_id":"322360f1c1","public_label":"Killer Whale (322360f1c1)","roles":["review"],"url":"https://sah.borca.ai/u/322360f1c1"}],"url":"https://sah.borca.ai/claims/cl_198bf72b675f379eeb7baf18cf72acf1"},{"public_id":"cl_2c99c69f3af2b20cbcb52becd8f4419d","status":"active","text":"Maximum denaturation temperature of proteins in synovial fluid varied according to disease severity classified by the Kellgren-Lawrence criterion for osteoarthritis.","confidence":0.88,"contributors":[{"id":171,"public_id":"b9tnx83g25","public_label":"eunsjani (b9tnx83g25)","roles":["extraction"],"url":"https://sah.borca.ai/u/b9tnx83g25"},{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["review"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":17,"public_id":"322360f1c1","public_label":"Killer Whale (322360f1c1)","roles":["review"],"url":"https://sah.borca.ai/u/322360f1c1"}],"url":"https://sah.borca.ai/claims/cl_2c99c69f3af2b20cbcb52becd8f4419d"},{"public_id":"cl_cf4fe8855fa7b806d2cfc25c5325917e","status":"active","text":"Temperature-dependent denaturing properties of proteins in synovial fluid measured by differential scanning calorimetry can inform the design of intra-articular lubricant additives that enhance protein stability and reduce joint wear.","confidence":0.72,"contributors":[{"id":171,"public_id":"b9tnx83g25","public_label":"eunsjani (b9tnx83g25)","roles":["extraction"],"url":"https://sah.borca.ai/u/b9tnx83g25"},{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["review"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":17,"public_id":"322360f1c1","public_label":"Killer Whale (322360f1c1)","roles":["review"],"url":"https://sah.borca.ai/u/322360f1c1"}],"url":"https://sah.borca.ai/claims/cl_cf4fe8855fa7b806d2cfc25c5325917e"}],"concepts":[{"public_id":"co_036306276c47517f3a937e9fb4f1ae5d","status":"active","name":"degenerative knee joint destruction","description":"End-stage structural deterioration of the knee joint included as one of the disease groups in the study.","types":["condition"],"aliases":[],"contributors":[{"id":171,"public_id":"b9tnx83g25","public_label":"eunsjani (b9tnx83g25)","roles":["extraction"],"url":"https://sah.borca.ai/u/b9tnx83g25"},{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["review"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":17,"public_id":"322360f1c1","public_label":"Killer Whale (322360f1c1)","roles":["review"],"url":"https://sah.borca.ai/u/322360f1c1"}],"url":"https://sah.borca.ai/concepts/co_036306276c47517f3a937e9fb4f1ae5d"},{"public_id":"co_1acc0e0fc207cf77e80d3b1f35267088","status":"active","name":"differential scanning calorimetry","description":"A thermoanalytical technique used here to measure heat flow during protein denaturation in synovial fluid samples.","types":["method"],"aliases":["DSC"],"contributors":[{"id":171,"public_id":"b9tnx83g25","public_label":"eunsjani (b9tnx83g25)","roles":["extraction"],"url":"https://sah.borca.ai/u/b9tnx83g25"},{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["review"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":17,"public_id":"322360f1c1","public_label":"Killer Whale (322360f1c1)","roles":["review"],"url":"https://sah.borca.ai/u/322360f1c1"}],"url":"https://sah.borca.ai/concepts/co_1acc0e0fc207cf77e80d3b1f35267088"},{"public_id":"co_1dbc943b0fc8f5b4a06b3bfa5f9e0ba3","status":"active","name":"SETARAM Micro DSC-II","description":"The specific microcalorimeter instrument used in this study at a scanning rate of 0.3 K/min over a 20–90 °C range.","types":["instrument"],"aliases":[],"contributors":[{"id":171,"public_id":"b9tnx83g25","public_label":"eunsjani (b9tnx83g25)","roles":["extraction"],"url":"https://sah.borca.ai/u/b9tnx83g25"},{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["review"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":17,"public_id":"322360f1c1","public_label":"Killer Whale (322360f1c1)","roles":["review"],"url":"https://sah.borca.ai/u/322360f1c1"}],"url":"https://sah.borca.ai/concepts/co_1dbc943b0fc8f5b4a06b3bfa5f9e0ba3"},{"public_id":"co_29b5ee2cf9368cf733d3d6fb6e374987","status":"active","name":"Staphylococcus aureus","description":"A gram-positive pathogenic bacterium used at 10⁻³ CFU to inoculate normal synovial fluid samples in this study.","types":["organism"],"aliases":[],"contributors":[{"id":171,"public_id":"b9tnx83g25","public_label":"eunsjani (b9tnx83g25)","roles":["extraction"],"url":"https://sah.borca.ai/u/b9tnx83g25"},{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["review"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":17,"public_id":"322360f1c1","public_label":"Killer Whale (322360f1c1)","roles":["review"],"url":"https://sah.borca.ai/u/322360f1c1"}],"url":"https://sah.borca.ai/concepts/co_29b5ee2cf9368cf733d3d6fb6e374987"},{"public_id":"co_52659b0737533d9ae7c20a249a526f98","status":"active","name":"Kellgren-Lawrence criterion","description":"A radiological grading scale for osteoarthritis severity used in this study to classify the degree of joint damage.","types":["classification system"],"aliases":["Kellgren-Lawrence scale"],"contributors":[{"id":171,"public_id":"b9tnx83g25","public_label":"eunsjani (b9tnx83g25)","roles":["extraction"],"url":"https://sah.borca.ai/u/b9tnx83g25"},{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["review"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":17,"public_id":"322360f1c1","public_label":"Killer Whale (322360f1c1)","roles":["review"],"url":"https://sah.borca.ai/u/322360f1c1"}],"url":"https://sah.borca.ai/concepts/co_52659b0737533d9ae7c20a249a526f98"},{"public_id":"co_54aab0458b0a2a383644287b1b775344","status":"active","name":"intra-articular lubricant additives","description":"Proposed therapeutic agents injected into the joint space to enhance protein stability and reduce articular surface wear.","types":["therapeutic application"],"aliases":[],"contributors":[{"id":171,"public_id":"b9tnx83g25","public_label":"eunsjani (b9tnx83g25)","roles":["extraction"],"url":"https://sah.borca.ai/u/b9tnx83g25"},{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["review"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":17,"public_id":"322360f1c1","public_label":"Killer Whale (322360f1c1)","roles":["review"],"url":"https://sah.borca.ai/u/322360f1c1"}],"url":"https://sah.borca.ai/concepts/co_54aab0458b0a2a383644287b1b775344"},{"public_id":"co_68507ca9c990ac036527cfd4b6d726b2","status":"active","name":"synovial fluid","description":"The protein-containing viscous fluid found in joint cavities, analyzed here as a diagnostic medium reflecting joint pathology.","types":["biological material"],"aliases":["SF"],"contributors":[{"id":171,"public_id":"b9tnx83g25","public_label":"eunsjani (b9tnx83g25)","roles":["extraction"],"url":"https://sah.borca.ai/u/b9tnx83g25"},{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["review"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":17,"public_id":"322360f1c1","public_label":"Killer Whale (322360f1c1)","roles":["review"],"url":"https://sah.borca.ai/u/322360f1c1"}],"url":"https://sah.borca.ai/concepts/co_68507ca9c990ac036527cfd4b6d726b2"},{"public_id":"co_6fb76c8d0b13bde5d50848bff4919534","status":"active","name":"Staphylococcus epidermidis","description":"A gram-positive bacterium used at 10⁻³ CFU alongside S. aureus to inoculate normal synovial fluid samples.","types":["organism"],"aliases":[],"contributors":[{"id":171,"public_id":"b9tnx83g25","public_label":"eunsjani (b9tnx83g25)","roles":["extraction"],"url":"https://sah.borca.ai/u/b9tnx83g25"},{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["review"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":17,"public_id":"322360f1c1","public_label":"Killer Whale (322360f1c1)","roles":["review"],"url":"https://sah.borca.ai/u/322360f1c1"}],"url":"https://sah.borca.ai/concepts/co_6fb76c8d0b13bde5d50848bff4919534"},{"public_id":"co_705dca45656b706245b0d308f07cafca","status":"active","name":"protein denaturation temperature","description":"The maximum temperature at which proteins in synovial fluid unfold, used here as an indicator of joint disease severity.","types":["measurement"],"aliases":["maximum denaturation temperature"],"contributors":[{"id":171,"public_id":"b9tnx83g25","public_label":"eunsjani (b9tnx83g25)","roles":["extraction"],"url":"https://sah.borca.ai/u/b9tnx83g25"},{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["review"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":17,"public_id":"322360f1c1","public_label":"Killer Whale (322360f1c1)","roles":["review"],"url":"https://sah.borca.ai/u/322360f1c1"}],"url":"https://sah.borca.ai/concepts/co_705dca45656b706245b0d308f07cafca"},{"public_id":"co_9097c8fbdd4d41efd081392343e45f9b","status":"active","name":"knee joint meniscus injuries","description":"Acute traumatic damage to the meniscal cartilage of the knee, one of the three joint conditions included in the 28-case study.","types":["condition"],"aliases":["acute knee joint meniscus injuries"],"contributors":[{"id":171,"public_id":"b9tnx83g25","public_label":"eunsjani (b9tnx83g25)","roles":["extraction"],"url":"https://sah.borca.ai/u/b9tnx83g25"},{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["review"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":17,"public_id":"322360f1c1","public_label":"Killer Whale (322360f1c1)","roles":["review"],"url":"https://sah.borca.ai/u/322360f1c1"}],"url":"https://sah.borca.ai/concepts/co_9097c8fbdd4d41efd081392343e45f9b"},{"public_id":"co_9f6908c892edc7a9ae71d3f1e8cdea57","status":"active","name":"transient knee joint synovitis","description":"A temporary inflammatory condition of the knee joint synovium, one of the conditions studied in the 28-case cohort.","types":["condition"],"aliases":[],"contributors":[{"id":171,"public_id":"b9tnx83g25","public_label":"eunsjani (b9tnx83g25)","roles":["extraction"],"url":"https://sah.borca.ai/u/b9tnx83g25"},{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["review"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":17,"public_id":"322360f1c1","public_label":"Killer Whale (322360f1c1)","roles":["review"],"url":"https://sah.borca.ai/u/322360f1c1"}],"url":"https://sah.borca.ai/concepts/co_9f6908c892edc7a9ae71d3f1e8cdea57"},{"public_id":"co_c5c42d91bc4b97967137eda26cc3fabb","status":"active","name":"DSC scan decomposition","description":"Mathematical deconvolution of DSC thermograms used here to separate and quantify individual protein denaturation contributions in synovial fluid.","types":["data analysis method"],"aliases":[],"contributors":[{"id":171,"public_id":"b9tnx83g25","public_label":"eunsjani (b9tnx83g25)","roles":["extraction"],"url":"https://sah.borca.ai/u/b9tnx83g25"},{"id":2,"public_id":"4715169a40","public_label":"AK (4715169a40)","roles":["review"],"url":"https://sah.borca.ai/u/4715169a40"},{"id":17,"public_id":"322360f1c1","public_label":"Killer Whale (322360f1c1)","roles":["review"],"url":"https://sah.borca.ai/u/322360f1c1"}],"url":"https://sah.borca.ai/concepts/co_c5c42d91bc4b97967137eda26cc3fabb"}],"external_ids":{"DOI":"10.1007/s10973-019-08151-6","ArXiv":null,"PubMed":null,"PubMedCentral":null,"MAG":2920417293,"DBLP":null,"ACL":null},"open_access":{"is_open_access":true,"pdf_url":"https://link.springer.com/content/pdf/10.1007/s10973-019-08151-6.pdf","landing_url":"https://www.semanticscholar.org/paper/0ef0a1e5d8ee7d0a58a866033e7f7055d9dd7df6","source":"semantic_scholar","pdf_url_source":"semantic_scholar_open_access_pdf","license":"CCBY","status":"HYBRID","reason":null},"reference_availability":{"status":"available","references_indexed":true,"full_text_available":false,"full_text_source":null,"count_basis":"semantic_scholar_metadata","extraction_status":"not_applicable","reason":null},"source":{"provider":"episteme2","base_corpus":"semantic_scholar_dump","freshness_mode":"unknown","basis":["semantic_scholar_metadata","postgres_metadata"],"limits":["paper metadata is based on indexed upstream scholarly datasets","claims and concepts are available only for extracted papers","absence of claims or concepts means no extracted graph data is available in this response"],"status":"available","degraded":false,"degraded_reasons":[],"diagnostics":{"status":"available","degraded":false,"degraded_reasons":[],"metadata_status":"available","graph_status":"available","abstract_status":"available"},"source_flags":1},"paper_id":631519,"paper_uid":"f41b3f45-11ac-45ae-bc20-53cd72bc64f4","canonical_identity":{"paper_id":631519,"paper_uid":"f41b3f45-11ac-45ae-bc20-53cd72bc64f4","identity_status":"available","lookup_basis":"semantic_scholar_external_id","compatibility_path":"corpus_id"},"url":"https://sah.borca.ai/papers/104466220"}