{"corpus_id":105396322,"paper_sha":"51e00df00a95b76452b7bb01a0d9d90acda257d0","doi":"10.1080/10889868.2018.1516619","arxiv_id":null,"pmid":null,"pmcid":null,"mag_id":2900970040,"dblp_id":null,"acl_id":null,"title":"Tendency of using different aromatic compounds as substrates by 2,4-DNT dioxygenase expressed by pJS39 carrying the gene dntA from Burkholderia sp. strain DNT","year":2018,"publication_date":"2018-11-13","venue":"Bioremediation Journal","journal":{"name":"Bioremediation Journal","pages":"22 - 31","volume":"23"},"journal_issn":null,"journal_title":null,"publication_types":[],"pubmed_pub_types":null,"s2_fields_of_study":["Biology","Chemistry","Environmental Science"],"reference_count":45,"citation_count":8,"influential_citation_count":0,"is_open_access":false,"arxiv_categories":null,"arxiv_license":null,"arxiv_journal_ref":null,"mesh_headings":null,"chemicals":null,"comments_corrections":null,"source_flags":1,"s2_open_access_pdf_url":null,"s2_open_access_landing_url":null,"s2_open_access_license":null,"s2_open_access_status":null,"pmc_open_access_pdf_url":null,"pmc_open_access_landing_url":null,"pmc_open_access_license":null,"pmc_open_access_status":null,"unpaywall_open_access_pdf_url":null,"unpaywall_open_access_landing_url":null,"unpaywall_open_access_license":null,"unpaywall_open_access_status":null,"abstract":"Abstract The substrate range of 2,4-dinitrotoluene (DNT) dioxygenase was investigated by measuring substrate-dependent O2 uptake and maximum growth (expressed in A600) on substrate-containing minimal medium. The control for each strain had no added particular substrate. The following aromatic compounds: catechol, α-naphthalene acetic acid, β-dimethylaminobenzaldehyde, 3,4-dinitrosalicylic acid, p-nitrophenol, naphthanol, o-anisic acid, salicylic acid, toluene, and benzoic acid, were tried as possible substrates. Considering all substrates used, only p-nitrophenol showed zero oxygen uptake rate and zero growth. This indicates that it was rather unlikely that p-nitrophenol is a substrate analog for 2,4-DNT. Catechol was clearly used as a sole carbon source by both wild-type Escherichia. coli (JM103) and the dnt transformant (JS39). Using α-naphthalene acetic acid and β-dimethylaminobenzaldehyde as substrates resulted in DNT dioxygenase oxygen uptake rates of 11.8 and 14 μM/hr/mg protein, respectively. However, using both compounds as a carbon source, JS39 had twice the growth rate of E. coli JM103. For the remaining six substrates tested (3, 4-dinitrosalicylic acid, p-nitrophenol, o-anisic acid, salicylic acid, toluene, and benzoic acid), there appeared to be growth advantages for JS39 (even though the growth in the presence of substrate was less than the controls) suggesting a situation similar to that described for α-naphthalene and β-dimethylaminobenzaldehyde above. Combining results from our assay with respirometry and growth-based experiments will allow a better understanding of the biochemical consequences of these interactions. These results suggest that DNT dioxygenase gene, dntA carried by JS39, and those potential genes for substrates-degraded enzyme(s) system could have a common root.","claims":[{"public_id":"cl_4090b10a9f7b33b5d309d377300fbecd","status":"active","text":"Catechol was used as a sole carbon source by both Escherichia coli JM103 and the dnt transformant JS39.","confidence":0.95,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_4090b10a9f7b33b5d309d377300fbecd"},{"public_id":"cl_73e95e2cb5664a48b95447b63e89216c","status":"active","text":"For the remaining six tested substrates, JS39 showed growth advantages relative to E. coli JM103, suggesting interactions similar to those observed for α-naphthalene acetic acid and β-dimethylaminobenzaldehyde.","confidence":0.88,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous 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(12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_6db9650d06adfe116fec5f3ecff1b807"},{"public_id":"cl_9d203f13e225e8f9772b147c785ba258","status":"active","text":"p-nitrophenol showed no oxygen uptake and no growth, making it unlikely to be a substrate analog for 2,4-DNT dioxygenase.","confidence":0.97,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_9d203f13e225e8f9772b147c785ba258"},{"public_id":"cl_bb29d0c2cc4fc1775af1a33ee393fba8","status":"active","text":"α-naphthalene acetic acid and β-dimethylaminobenzaldehyde produced DNT dioxygenase oxygen uptake rates of 11.8 and 14 μM/hr/mg protein, respectively.","confidence":0.94,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous 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