{"corpus_id":12339111,"paper_sha":"fc3b1ba27e7f9e98ee97c32cd88abc97979879ab","doi":"10.1016/j.biomaterials.2009.05.061","arxiv_id":null,"pmid":19540579,"pmcid":"PMC3119364","mag_id":2179257547,"dblp_id":null,"acl_id":null,"title":"The influence of tethered epidermal growth factor on connective tissue progenitor colony formation.","year":2009,"publication_date":"2009-09-01","venue":"Biomaterials","journal":{"name":"Biomaterials","pages":"\n          4629-38\n        ","volume":"30 27"},"journal_issn":null,"journal_title":null,"publication_types":["JournalArticle"],"pubmed_pub_types":["Journal Article","Research Support, N.I.H., Extramural"],"s2_fields_of_study":["Biology","Medicine","Engineering"],"reference_count":57,"citation_count":40,"influential_citation_count":3,"is_open_access":true,"arxiv_categories":null,"arxiv_license":null,"arxiv_journal_ref":null,"mesh_headings":[{"d":"Adsorption","mj":false,"qs":[{"q":"drug effects","mj":false,"ui":"Q000187"}],"ui":"D000327"},{"d":"Cell Adhesion","mj":false,"qs":[{"q":"drug effects","mj":false,"ui":"Q000187"}],"ui":"D002448"},{"d":"Cell Count","mj":false,"ui":"D002452"},{"d":"Cell Differentiation","mj":false,"qs":[{"q":"drug effects","mj":false,"ui":"Q000187"}],"ui":"D002454"},{"d":"Cell Size","mj":false,"qs":[{"q":"drug effects","mj":false,"ui":"Q000187"}],"ui":"D048429"},{"d":"Colony-Forming Units Assay","mj":true,"ui":"D003114"},{"d":"Connective Tissue Cells","mj":false,"qs":[{"q":"cytology","mj":true,"ui":"Q000166"},{"q":"drug effects","mj":true,"ui":"Q000187"}],"ui":"D003239"},{"d":"Epidermal Growth Factor","mj":false,"qs":[{"q":"pharmacology","mj":true,"ui":"Q000494"}],"ui":"D004815"},{"d":"Fibronectins","mj":false,"qs":[{"q":"pharmacology","mj":false,"ui":"Q000494"}],"ui":"D005353"},{"d":"Humans","mj":false,"ui":"D006801"},{"d":"Osteogenesis","mj":false,"qs":[{"q":"drug effects","mj":false,"ui":"Q000187"}],"ui":"D010012"},{"d":"Peptides","mj":false,"qs":[{"q":"pharmacology","mj":false,"ui":"Q000494"}],"ui":"D010455"},{"d":"Polymers","mj":false,"ui":"D011108"},{"d":"Serum","mj":false,"ui":"D044967"},{"d":"Solubility","mj":false,"qs":[{"q":"drug effects","mj":false,"ui":"Q000187"}],"ui":"D012995"},{"d":"Stem Cells","mj":false,"qs":[{"q":"cytology","mj":true,"ui":"Q000166"},{"q":"drug effects","mj":true,"ui":"Q000187"}],"ui":"D013234"}],"chemicals":[{"n":"Fibronectins","ui":"D005353","reg":"0"},{"n":"Peptides","ui":"D010455","reg":"0"},{"n":"Polymers","ui":"D011108","reg":"0"},{"n":"Epidermal Growth Factor","ui":"D004815","reg":"62229-50-9"}],"comments_corrections":null,"source_flags":5,"s2_open_access_pdf_url":"https://dspace.mit.edu/bitstream/1721.1/99188/1/Griffith_Influence%20tethered.pdf","s2_open_access_landing_url":"https://www.semanticscholar.org/paper/fc3b1ba27e7f9e98ee97c32cd88abc97979879ab","s2_open_access_license":"CCBYNCND","s2_open_access_status":"GREEN","pmc_open_access_pdf_url":null,"pmc_open_access_landing_url":null,"pmc_open_access_license":null,"pmc_open_access_status":null,"unpaywall_open_access_pdf_url":null,"unpaywall_open_access_landing_url":null,"unpaywall_open_access_license":null,"unpaywall_open_access_status":null,"abstract":"Strategies to combine aspirated marrow cells with scaffolds to treat connective tissue defects are gaining increasing clinical attention and use. In situations such as large defects where initial survival and proliferation of transplanted connective tissue progenitors (CTPs) are limiting, therapeutic outcomes might be improved by using the scaffold to deliver growth factors that promote the early stages of cell function in the graft. Signaling by the epidermal growth factor receptor (EGFR) plays a role in cell survival and has been implicated in bone development and homeostasis. Providing epidermal growth factor (EGF) in a scaffold-tethered format may sustain local delivery and shift EGFR signaling to pro-survival modes compared to soluble ligand. We therefore examined the effect of tethered EGF on osteogenic colony formation from human bone marrow aspirates in the context of three different adhesion environments using a total of 39 donors. We found that tethered EGF, but not soluble EGF, increased the numbers of colonies formed regardless of adhesion background, and that tethered EGF did not impair early stages of osteogenic differentiation.","claims":[{"public_id":"cl_2a9939da5c7f24caf257b302873d1e46","status":"active","text":"Soluble EGF did not increase colony numbers under the tested adhesion conditions.","confidence":0.93,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_2a9939da5c7f24caf257b302873d1e46"},{"public_id":"cl_1e6a425ea6d052d8d41a523d8cf9ce8b","status":"active","text":"Tethered EGF did not impair the early stages of osteogenic differentiation.","confidence":0.96,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_1e6a425ea6d052d8d41a523d8cf9ce8b"},{"public_id":"cl_08b09ec8add3f53b0716cd901b607b0e","status":"active","text":"Tethered EGF increased the number of colonies formed from human bone marrow aspirates across all three adhesion environments.","confidence":0.98,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_08b09ec8add3f53b0716cd901b607b0e"}],"concepts":[{"public_id":"co_2dbf0f42c7760fcb98d4ac6a1376d5b6","status":"active","name":"osteogenic differentiation","description":"The process by which progenitor cells begin developing toward an osteoblast lineage.","types":["cellular process"],"aliases":["early stages of osteogenic differentiation"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_2dbf0f42c7760fcb98d4ac6a1376d5b6"},{"public_id":"co_70ef9a4481655bf9a3d7479fcd8fae63","status":"active","name":"soluble EGF","description":"Epidermal growth factor delivered in free, non-tethered form.","types":["growth factor"],"aliases":["soluble epidermal growth factor"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_70ef9a4481655bf9a3d7479fcd8fae63"},{"public_id":"co_c91d0ab6bcb2a5be19c44fa03a7405e1","status":"active","name":"adhesion environments","description":"Different substrate adhesion conditions used to test colony formation.","types":["experimental condition"],"aliases":["adhesion background"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_c91d0ab6bcb2a5be19c44fa03a7405e1"},{"public_id":"co_caf6f9bdefb90bc9761cd43b6383af14","status":"active","name":"tethered epidermal growth factor","description":"Epidermal growth factor presented in a scaffold-bound format for localized delivery.","types":["growth factor"],"aliases":["tethered EGF"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_caf6f9bdefb90bc9761cd43b6383af14"},{"public_id":"co_dc2e681e0ec39cd0485d862b79ebb6cc","status":"active","name":"human bone marrow aspirates","description":"Bone marrow samples collected by aspiration from human donors.","types":["biological sample"],"aliases":["marrow aspirates"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_dc2e681e0ec39cd0485d862b79ebb6cc"},{"public_id":"co_eaee066a01ccedca18ef56e221f4edee","status":"active","name":"osteogenic colony formation","description":"The formation of colonies with osteogenic potential from marrow-derived progenitor cells.","types":["cellular outcome"],"aliases":["colony formation"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_eaee066a01ccedca18ef56e221f4edee"}],"external_ids":{"DOI":"10.1016/j.biomaterials.2009.05.061","ArXiv":null,"PubMed":19540579,"PubMedCentral":"PMC3119364","MAG":2179257547,"DBLP":null,"ACL":null},"open_access":{"is_open_access":true,"pdf_url":"https://dspace.mit.edu/bitstream/1721.1/99188/1/Griffith_Influence%20tethered.pdf","landing_url":"https://www.semanticscholar.org/paper/fc3b1ba27e7f9e98ee97c32cd88abc97979879ab","source":"semantic_scholar","pdf_url_source":"semantic_scholar_open_access_pdf","license":"CCBYNCND","status":"GREEN","reason":null},"reference_availability":{"status":"available","references_indexed":true,"full_text_available":false,"full_text_source":null,"count_basis":"semantic_scholar_metadata","extraction_status":"not_applicable","reason":null},"source":{"provider":"episteme2","base_corpus":"semantic_scholar_dump","freshness_mode":"unknown","basis":["semantic_scholar_metadata","postgres_metadata"],"limits":["paper metadata is based on indexed upstream scholarly datasets","claims and concepts are available only for extracted papers","absence of claims or concepts means no extracted graph data is available in this response"],"status":"available","degraded":false,"degraded_reasons":[],"diagnostics":{"status":"available","degraded":false,"degraded_reasons":[],"metadata_status":"available","graph_status":"available","abstract_status":"available"},"source_flags":5},"paper_id":632172,"paper_uid":"59358f83-c456-474c-8112-4f4dd4e6b192","canonical_identity":{"paper_id":632172,"paper_uid":"59358f83-c456-474c-8112-4f4dd4e6b192","identity_status":"available","lookup_basis":"semantic_scholar_external_id","compatibility_path":"corpus_id"},"url":"https://sah.borca.ai/papers/12339111"}