{"corpus_id":127952322,"paper_sha":"425b5215cc17b43de681007188d2a34dc76e01e7","doi":"10.1016/j.optlaseng.2020.106003","arxiv_id":"1904.09386","pmid":null,"pmcid":null,"mag_id":3004360950,"dblp_id":null,"acl_id":null,"title":"Wide-field high-resolution 3D microscopy with Fourier ptychographic diffraction tomography","year":2019,"publication_date":"2019-04-20","venue":"Optics and lasers in engineering","journal":{"name":"Optics and Lasers in Engineering","pages":null,"volume":null},"journal_issn":null,"journal_title":null,"publication_types":[],"pubmed_pub_types":null,"s2_fields_of_study":["Materials Science","Physics","Engineering"],"reference_count":65,"citation_count":186,"influential_citation_count":2,"is_open_access":false,"arxiv_categories":["physics.optics","physics.bio-ph"],"arxiv_license":"http://creativecommons.org/licenses/by/4.0/","arxiv_journal_ref":null,"mesh_headings":null,"chemicals":null,"comments_corrections":null,"source_flags":1,"s2_open_access_pdf_url":null,"s2_open_access_landing_url":null,"s2_open_access_license":null,"s2_open_access_status":null,"pmc_open_access_pdf_url":null,"pmc_open_access_landing_url":null,"pmc_open_access_license":null,"pmc_open_access_status":null,"unpaywall_open_access_pdf_url":null,"unpaywall_open_access_landing_url":null,"unpaywall_open_access_license":null,"unpaywall_open_access_status":null,"abstract":"Abstract We report a computational 3D microscopy technique, termed Fourier ptychographic diffraction tomography (FPDT), that iteratively stitches together numerous variably illuminated, low-resolution images acquired with a low-numerical aperture (NA) objective in 3D Fourier space to create a wide field-of-view (FOV), high-resolution, depth-resolved complex refractive index (RI) image across large volumes. Unlike conventional optical diffraction tomography (ODT) approaches that rely on controlled bright-field illumination, holographic phase measurement, and high-NA objective detection, FPDT employs tomographic RI reconstruction from low-NA intensity-only measurements. In addition, FPDT incorporates high-angle dark-field illuminations beyond the NA of the objective, significantly expanding the accessible object frequency. With FPDT, we present the highest-throughput ODT results with 390 nm lateral resolution and 899 nm axial resolution across a 10 ×  FOV of 1.77 mm2 and a depth of focus of  ~ 20 µm. Billion-voxel 3D tomographic imaging results of biological samples establish FPDT as a powerful non-invasive and label-free tool for high-throughput 3D microscopy applications.","claims":[{"public_id":"cl_9ce0f3bfa11da5ea2a4799f03e9c227f","status":"active","text":"Billion-voxel 3D tomographic imaging of biological samples demonstrates FPDT as a non-invasive, label-free tool for high-throughput 3D microscopy.","confidence":0.96,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_9ce0f3bfa11da5ea2a4799f03e9c227f"},{"public_id":"cl_f2c30343c3ec8dc6b2fac99c9ba2ba5f","status":"active","text":"Fourier ptychographic diffraction tomography reconstructs a wide-field, high-resolution, depth-resolved complex refractive index image by iteratively stitching variably illuminated low-resolution images in 3D Fourier 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detection.","confidence":0.95,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_b3b2b6060ee0521a737dc35b5667244c"},{"public_id":"cl_6c57c0c32711423a36564f85ccaa89f2","status":"active","text":"The reported system achieves 390 nm lateral resolution, 899 nm axial resolution, a 1.77 mm2 field of view, and a depth of focus of about 20 µm, representing the highest-throughput optical diffraction tomography results presented.","confidence":0.97,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_6c57c0c32711423a36564f85ccaa89f2"}],"concepts":[{"public_id":"co_0d1f95d4825980f55e292afe4e852337","status":"active","name":"objective numerical aperture","description":"The numerical aperture limit of the imaging 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