{"corpus_id":21074,"paper_sha":"74bb33c99da5e4271fc361c233f18d8064a0289f","doi":"10.1083/JCB.99.6.2034","arxiv_id":null,"pmid":6501412,"pmcid":"2113570","mag_id":2098400443,"dblp_id":null,"acl_id":null,"title":"Identification and isolation of endothelial cells based on their increased uptake of acetylated-low density lipoprotein","year":1984,"publication_date":"1984-12-01","venue":"Journal of Cell Biology","journal":{"name":"The Journal of Cell Biology","pages":"2034 - 2040","volume":"99"},"journal_issn":null,"journal_title":null,"publication_types":["JournalArticle"],"pubmed_pub_types":["Journal Article","Research Support, Non-U.S. Gov't","Research Support, U.S. Gov't, P.H.S."],"s2_fields_of_study":["Biology","Medicine"],"reference_count":29,"citation_count":1234,"influential_citation_count":25,"is_open_access":true,"arxiv_categories":null,"arxiv_license":null,"arxiv_journal_ref":null,"mesh_headings":[{"d":"Acetylation","mj":false,"ui":"D000107"},{"d":"Animals","mj":false,"ui":"D000818"},{"d":"Aorta","mj":false,"qs":[{"q":"cytology","mj":true,"ui":"Q000166"},{"q":"metabolism","mj":false,"ui":"Q000378"}],"ui":"D001011"},{"d":"Cattle","mj":false,"ui":"D002417"},{"d":"Cell Separation","mj":false,"qs":[{"q":"methods","mj":false,"ui":"Q000379"}],"ui":"D002469"},{"d":"Cells, Cultured","mj":false,"ui":"D002478"},{"d":"Endothelium","mj":false,"qs":[{"q":"cytology","mj":true,"ui":"Q000166"},{"q":"metabolism","mj":false,"ui":"Q000378"}],"ui":"D004727"},{"d":"Kinetics","mj":false,"ui":"D007700"},{"d":"Lipoproteins, LDL","mj":false,"qs":[{"q":"metabolism","mj":true,"ui":"Q000378"}],"ui":"D008077"},{"d":"Muscle, Smooth, Vascular","mj":false,"qs":[{"q":"cytology","mj":false,"ui":"Q000166"},{"q":"metabolism","mj":false,"ui":"Q000378"}],"ui":"D009131"},{"d":"Receptors, LDL","mj":false,"qs":[{"q":"metabolism","mj":false,"ui":"Q000378"}],"ui":"D011973"}],"chemicals":[{"n":"Lipoproteins, LDL","ui":"D008077","reg":"0"},{"n":"Receptors, LDL","ui":"D011973","reg":"0"},{"n":"acetyl-LDL","ui":"C032629","reg":"0"}],"comments_corrections":null,"source_flags":5,"s2_open_access_pdf_url":"http://jcb.rupress.org/content/jcb/99/6/2034.full.pdf","s2_open_access_landing_url":"https://www.semanticscholar.org/paper/74bb33c99da5e4271fc361c233f18d8064a0289f","s2_open_access_license":"CCBYNCSA","s2_open_access_status":"BRONZE","pmc_open_access_pdf_url":null,"pmc_open_access_landing_url":null,"pmc_open_access_license":null,"pmc_open_access_status":null,"unpaywall_open_access_pdf_url":null,"unpaywall_open_access_landing_url":null,"unpaywall_open_access_license":null,"unpaywall_open_access_status":null,"abstract":"Acetylated-low density lipoprotein (Ac-LDL) is taken up by macrophages and endothelial cells via the \"scavenger cell pathway\" of LDL metabolism. In this report, aortic and microvascular endothelial cells internalized and degraded 7-15 times more [125I]-Ac-LDL than did smooth muscle cells or pericytes. Bound [125I]-Ac-LDL was displaced by unlabeled Ac-LDL, but not unmodified LDL. The ability to identify endothelial cells based on their increased metabolism of Ac-LDL was examined using Ac-LDL labeled with the fluorescent probe 1,1'- dioctadecyl-3,3,3',3'-tetramethyl-indocarbocyanine perchlorate (Dil-Ac- LDL). When cells were incubated with 10 micrograms/ml Dil-Ac-LDL for 4 h at 37 degrees C and subsequently examined by fluorescence microscopy, capillary and aortic endothelial cells were brilliantly fluorescent whereas the fluorescent intensity of retinal pericytes and smooth muscle cells was only slightly above background levels. Dil-Ac-LDL at the concentration used for labeling cells had no effect on endothelial cell growth rate. When primary cultures of bovine adrenal capillary cells were labeled with 10 micrograms/ml of Dil-Ac-LDL for 4 h at 37 degrees C, then trypsinized and subjected to fluorescence-activated cell sorting, pure cultures of capillary endothelial cells could be obtained. Utilizing this method, large numbers of early passage microvascular endothelial cells can be obtained in significantly less time than with conventional methods.","claims":[{"public_id":"cl_aa2d6210321b499029312fa333620343","status":"active","text":"Aortic and microvascular endothelial cells internalized and degraded 7-15 times more [125I]-Ac-LDL than smooth muscle cells or pericytes.","confidence":0.98,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_aa2d6210321b499029312fa333620343"},{"public_id":"cl_be787b7764ad56dc966741893fb108aa","status":"active","text":"At 10 micrograms/ml for 4 h at 37 degrees C, Dil-Ac-LDL had no effect on endothelial cell growth rate and enabled fluorescence-activated cell sorting to yield pure cultures of capillary endothelial cells.","confidence":0.95,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_be787b7764ad56dc966741893fb108aa"},{"public_id":"cl_4446fedc849421488afa10fe7c8d8638","status":"active","text":"Binding of [125I]-Ac-LDL was displaced by unlabeled acetylated-low density lipoprotein but not by unmodified low density lipoprotein.","confidence":0.96,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_4446fedc849421488afa10fe7c8d8638"},{"public_id":"cl_93b73aa561ce37e0750ef38130ca5e2e","status":"active","text":"Dil-Ac-LDL labeling made capillary and aortic endothelial cells brightly fluorescent, while retinal pericytes and smooth muscle cells remained only slightly above background fluorescence.","confidence":0.97,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_93b73aa561ce37e0750ef38130ca5e2e"},{"public_id":"cl_c591472ca696228710170df0c956ddbb","status":"active","text":"The labeling approach provides a faster way to obtain large numbers of early passage microvascular endothelial cells than conventional methods.","confidence":0.88,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_c591472ca696228710170df0c956ddbb"}],"concepts":[{"public_id":"co_0f3264c6714585705e84cddd6bd988bc","status":"active","name":"aortic and microvascular endothelial cells","description":"Endothelial cells from large vessels and microvessels used in uptake and degradation experiments.","types":["cell 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