{"corpus_id":210975684,"paper_sha":"008aadd0ac8e76d2834c8874f170ea8480caefe9","doi":"10.1186/s12943-020-1134-8","arxiv_id":null,"pmid":32005118,"pmcid":"6993513","mag_id":3007300208,"dblp_id":null,"acl_id":null,"title":"Hsa_circ_001680 affects the proliferation and migration of CRC and mediates its chemoresistance by regulating BMI1 through miR-340","year":2020,"publication_date":"2020-01-31","venue":"Molecular Cancer","journal":{"name":"Molecular Cancer","pages":null,"volume":"19"},"journal_issn":null,"journal_title":null,"publication_types":["JournalArticle"],"pubmed_pub_types":["Journal Article","Research Support, Non-U.S. Gov't"],"s2_fields_of_study":["Biology","Medicine"],"reference_count":56,"citation_count":167,"influential_citation_count":4,"is_open_access":true,"arxiv_categories":null,"arxiv_license":null,"arxiv_journal_ref":null,"mesh_headings":[{"d":"Animals","mj":false,"ui":"D000818"},{"d":"Antineoplastic Agents, Phytogenic","mj":false,"qs":[{"q":"pharmacology","mj":false,"ui":"Q000494"}],"ui":"D000972"},{"d":"Apoptosis","mj":false,"ui":"D017209"},{"d":"Biomarkers, Tumor","mj":false,"qs":[{"q":"genetics","mj":false,"ui":"Q000235"},{"q":"metabolism","mj":true,"ui":"Q000378"}],"ui":"D014408"},{"d":"Cell Movement","mj":true,"ui":"D002465"},{"d":"Cell Proliferation","mj":false,"ui":"D049109"},{"d":"Colorectal Neoplasms","mj":false,"qs":[{"q":"drug therapy","mj":false,"ui":"Q000188"},{"q":"genetics","mj":false,"ui":"Q000235"},{"q":"metabolism","mj":false,"ui":"Q000378"},{"q":"pathology","mj":true,"ui":"Q000473"}],"ui":"D015179"},{"d":"Drug Resistance, Neoplasm","mj":true,"ui":"D019008"},{"d":"Female","mj":false,"ui":"D005260"},{"d":"Gene Expression Regulation, Neoplastic","mj":false,"ui":"D015972"},{"d":"Humans","mj":false,"ui":"D006801"},{"d":"Irinotecan","mj":false,"qs":[{"q":"pharmacology","mj":false,"ui":"Q000494"}],"ui":"D000077146"},{"d":"Mice","mj":false,"ui":"D051379"},{"d":"Mice, Inbred BALB C","mj":false,"ui":"D008807"},{"d":"Mice, Nude","mj":false,"ui":"D008819"},{"d":"MicroRNAs","mj":false,"qs":[{"q":"genetics","mj":true,"ui":"Q000235"}],"ui":"D035683"},{"d":"Polycomb Repressive Complex 1","mj":false,"qs":[{"q":"genetics","mj":false,"ui":"Q000235"},{"q":"metabolism","mj":true,"ui":"Q000378"}],"ui":"D063150"},{"d":"RNA, Circular","mj":false,"qs":[{"q":"genetics","mj":true,"ui":"Q000235"}],"ui":"D000079962"},{"d":"Tumor Cells, Cultured","mj":false,"ui":"D014407"},{"d":"Xenograft Model Antitumor Assays","mj":false,"ui":"D023041"}],"chemicals":[{"n":"Antineoplastic Agents, Phytogenic","ui":"D000972","reg":"0"},{"n":"BMI1 protein, human","ui":"C069077","reg":"0"},{"n":"Biomarkers, Tumor","ui":"D014408","reg":"0"},{"n":"MIRN340 microRNA, human","ui":"C570046","reg":"0"},{"n":"MicroRNAs","ui":"D035683","reg":"0"},{"n":"RNA, Circular","ui":"D000079962","reg":"0"},{"n":"Irinotecan","ui":"D000077146","reg":"7673326042"},{"n":"Polycomb Repressive Complex 1","ui":"D063150","reg":"EC 2.3.2.27"}],"comments_corrections":null,"source_flags":5,"s2_open_access_pdf_url":"https://molecular-cancer.biomedcentral.com/track/pdf/10.1186/s12943-020-1134-8.pdf","s2_open_access_landing_url":"https://www.semanticscholar.org/paper/008aadd0ac8e76d2834c8874f170ea8480caefe9","s2_open_access_license":"CCBY","s2_open_access_status":"GOLD","pmc_open_access_pdf_url":null,"pmc_open_access_landing_url":null,"pmc_open_access_license":null,"pmc_open_access_status":null,"unpaywall_open_access_pdf_url":null,"unpaywall_open_access_landing_url":null,"unpaywall_open_access_license":null,"unpaywall_open_access_status":null,"abstract":"Accumulating evidence indicates that circular RNAs (circRNAs) act as microRNA (miRNA) sponges to directly inhibit specific miRNAs and alter their ability to regulate gene expression at the post-transcriptional level; this mechanism is believed to occur in various cancers. However, the expression level, precise function and mechanism of circ_001680 in colorectal carcinoma (CRC) are largely unknown. qRT-PCR was used to detect the expression of circ_001680 and miR-340 in human CRC tissues and their matched normal tissues. Bioinformatics analyses and dual-fluorescence reporter assays were used to evaluate whether circ_001680 could bind to miR-340. Circ_001680 overexpression and knockdown cell lines were constructed to investigate the proliferation and migration abilities in vivo and in vitro through function-based experiments, including CCK8, plate clone formation, transwell, and wounding healing assays. The relationships among circ_001680, miR-340 and BMI1 were investigated by bioinformatics analyses, dual-fluorescence reporter system, FISH, RIP and RNA pull down assays. Sphere forming assays and flow cytometry analyses were used to assess the effect of circ_001680 on the stemness characteristics of CRC cells. Circ_001680 was more highly expressed in of CRC tissue than in matched adjacent normal tissues from the same patients. Circ_001680 was observed to enhance the proliferation and migration capacity of CRC cells. Furthermore, dual-fluorescence reporter assays confirmed that circ_001680 affects the expression of BMI1 by targeting miR-340. More importantly, we also found that circ_001680 could promote the cancer stem cell (CSC) population in CRC and induce irinotecan therapeutic resistance by regulating the miR-340 target gene BMI1. Our results demonstrated that circ_001680 is a part of a novel strategy to induce chemotherapy resistance in CRC through BMI1 upregulation. Moreover, circ_001680 may be a promising diagnostic and prognostic marker to determine the success of irinotecan-based chemotherapy.","claims":[{"public_id":"cl_58d66285039d1b9deacbb247f56eadc0","status":"active","text":"circ_001680 affects BMI1 expression by targeting miR-340.","confidence":0.96,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_58d66285039d1b9deacbb247f56eadc0"},{"public_id":"cl_da8eff26d4f0bf891b992429162f6aa3","status":"active","text":"circ_001680 enhances the proliferation and migration capacity of colorectal carcinoma cells.","confidence":0.98,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous 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