{"corpus_id":21122536,"paper_sha":"7746353d33208b354a42dbd27cec7a5b0150018c","doi":"10.1016/j.jviromet.2016.10.005","arxiv_id":null,"pmid":27751950,"pmcid":null,"mag_id":2538170161,"dblp_id":null,"acl_id":null,"title":"Preparation of viral samples within biocontainment for ultrastructural analysis: Utilization of an innovative processing capsule for negative staining.","year":2016,"publication_date":"2016-12-01","venue":"Journal of Virological Methods","journal":{"name":"Journal of virological methods","pages":"\n          70-76\n        ","volume":"238"},"journal_issn":null,"journal_title":null,"publication_types":["JournalArticle"],"pubmed_pub_types":["Journal Article"],"s2_fields_of_study":["Biology","Medicine","Materials Science"],"reference_count":18,"citation_count":18,"influential_citation_count":0,"is_open_access":false,"arxiv_categories":null,"arxiv_license":null,"arxiv_journal_ref":null,"mesh_headings":[{"d":"Chikungunya virus","mj":false,"qs":[{"q":"ultrastructure","mj":false,"ui":"Q000648"}],"ui":"D002646"},{"d":"Containment of Biohazards","mj":true,"qs":[{"q":"methods","mj":false,"ui":"Q000379"}],"ui":"D003264"},{"d":"Ebolavirus","mj":false,"qs":[{"q":"ultrastructure","mj":false,"ui":"Q000648"}],"ui":"D029043"},{"d":"Microscopy, Electron, Transmission","mj":false,"qs":[{"q":"instrumentation","mj":false,"ui":"Q000295"},{"q":"methods","mj":true,"ui":"Q000379"},{"q":"standards","mj":false,"ui":"Q000592"}],"ui":"D046529"},{"d":"Negative Staining","mj":false,"qs":[{"q":"methods","mj":true,"ui":"Q000379"}],"ui":"D016624"},{"d":"Specimen Handling","mj":false,"qs":[{"q":"methods","mj":true,"ui":"Q000379"}],"ui":"D013048"},{"d":"Viruses","mj":false,"qs":[{"q":"isolation & purification","mj":false,"ui":"Q000302"},{"q":"ultrastructure","mj":true,"ui":"Q000648"}],"ui":"D014780"}],"chemicals":null,"comments_corrections":null,"source_flags":5,"s2_open_access_pdf_url":null,"s2_open_access_landing_url":null,"s2_open_access_license":null,"s2_open_access_status":null,"pmc_open_access_pdf_url":null,"pmc_open_access_landing_url":null,"pmc_open_access_license":null,"pmc_open_access_status":null,"unpaywall_open_access_pdf_url":null,"unpaywall_open_access_landing_url":null,"unpaywall_open_access_license":null,"unpaywall_open_access_status":null,"abstract":"Transmission electron microscopy can be used to observe the ultrastructure of viruses and other microbial pathogens with nanometer resolution. In a transmission electron microscope (TEM), the image is created by passing an electron beam through a specimen with contrast generated by electron scattering from dense elements in the specimen. Viruses do not normally contain dense elements, so a negative stain that places dense heavy metal salts around the sample is added to create a dark border. To prepare a virus sample for a negative stain transmission electron microscopy, a virus suspension is applied to a TEM grid specimen support, which is a 3mm diameter fragile specimen screen coated with a few nanometers of plastic film. Then, deionized (dI) water rinses and a negative stain solution are applied to the grid. All infectious viruses must be handled in a biosafety cabinet (BSC) and many require a biocontainment laboratory environment. Staining viruses in biosafety levels (BSL) 3 and 4 is especially challenging because the support grids are small, fragile, and easily moved by air currents. In this study we evaluated a new device for negative staining viruses called mPrep/g capsule. It is a capsule that holds up to two TEM grids during all processing steps and for storage after staining is complete. This study reports that the mPrep/g capsule method is valid and effective to negative stain virus specimens, especially in high containment laboratory environments.","claims":[{"public_id":"cl_438cf566ef1063a3cd6d58f506263ff0","status":"active","text":"The capsule approach is especially useful in high containment laboratory environments.","confidence":0.9,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_438cf566ef1063a3cd6d58f506263ff0"},{"public_id":"cl_6d137dead52425c911935b5398889057","status":"active","text":"The capsule supports TEM grids through all processing steps and storage after staining is complete.","confidence":0.92,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous 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