{"corpus_id":214627928,"paper_sha":"1ab92ce8a6e375fbd557d7ab39f316bf086c497b","doi":"10.1016/j.phrs.2020.104761","arxiv_id":null,"pmid":32205232,"pmcid":"7102548","mag_id":3011090040,"dblp_id":null,"acl_id":null,"title":"Lianhuaqingwen exerts anti-viral and anti-inflammatory activity against novel coronavirus (SARS-CoV-2)","year":2020,"publication_date":"2020-03-20","venue":"Pharmacological Research","journal":{"name":"Pharmacological Research","pages":"104761 - 104761","volume":"156"},"journal_issn":null,"journal_title":null,"publication_types":["JournalArticle"],"pubmed_pub_types":["Journal Article","Research Support, Non-U.S. Gov't"],"s2_fields_of_study":["Medicine","Chemistry"],"reference_count":26,"citation_count":586,"influential_citation_count":39,"is_open_access":true,"arxiv_categories":null,"arxiv_license":null,"arxiv_journal_ref":null,"mesh_headings":[{"d":"Animals","mj":false,"ui":"D000818"},{"d":"Anti-Inflammatory Agents","mj":false,"qs":[{"q":"pharmacology","mj":true,"ui":"Q000494"}],"ui":"D000893"},{"d":"Antiviral Agents","mj":false,"qs":[{"q":"pharmacology","mj":true,"ui":"Q000494"}],"ui":"D000998"},{"d":"Betacoronavirus","mj":false,"qs":[{"q":"drug effects","mj":true,"ui":"Q000187"},{"q":"ultrastructure","mj":false,"ui":"Q000648"}],"ui":"D000073640"},{"d":"Cell Line","mj":false,"ui":"D002460"},{"d":"Chlorocebus aethiops","mj":false,"ui":"D002522"},{"d":"Drugs, Chinese Herbal","mj":false,"qs":[{"q":"pharmacology","mj":true,"ui":"Q000494"}],"ui":"D004365"},{"d":"Microscopy, Electrochemical, Scanning","mj":false,"ui":"D064347"},{"d":"SARS-CoV-2","mj":false,"ui":"D000086402"}],"chemicals":[{"n":"Anti-Inflammatory Agents","ui":"D000893","reg":"0"},{"n":"Antiviral Agents","ui":"D000998","reg":"0"},{"n":"Drugs, Chinese Herbal","ui":"D004365","reg":"0"},{"n":"lianhuaqingwen","ui":"C000588632","reg":"0"}],"comments_corrections":null,"source_flags":5,"s2_open_access_pdf_url":"https://europepmc.org/articles/pmc7102548?pdf=render","s2_open_access_landing_url":"https://www.semanticscholar.org/paper/1ab92ce8a6e375fbd557d7ab39f316bf086c497b","s2_open_access_license":"unspecified-oa","s2_open_access_status":"GREEN","pmc_open_access_pdf_url":null,"pmc_open_access_landing_url":null,"pmc_open_access_license":null,"pmc_open_access_status":null,"unpaywall_open_access_pdf_url":null,"unpaywall_open_access_landing_url":null,"unpaywall_open_access_license":null,"unpaywall_open_access_status":null,"abstract":"Purpose Lianhuaqingwen (LH) as traditional Chinese medicine (TCM) formula has been used to treat influenza and exerted broad-spectrum antiviral effects on a series of influenza viruses and immune regulatory effects Ding et al. (2017). The goal of this study is to demonstrate the antiviral activity of LH against the novel SARS-CoV-2 virus and its potential effect in regulating host immune response. Methods The antiviral activity of LH against SARS-CoV-2 was assessed in Vero E6 cells using CPE and plaque reduction assay. The effect of LH on virion morphology was visualized under transmission electron microscope. Pro-inflammatory cytokine expression levels upon SARS-CoV-2 infection in Huh-7 cells were measured by real-time quantitative PCR assays. Results LH significantly inhibited SARS-CoV-2 replication in Vero E6 cells and markedly reduced pro-inflammatory cytokines (TNF-α, IL-6, CCL-2/MCP-1 and CXCL-10/IP-10) production at the mRNA levels. Furthermore, LH treatment resulted in abnormal particle morphology of virion in cells. Conclusions LH significantly inhibits the SARS-COV-2 replication, affects virus morphology and exerts anti-inflammatory activity in vitro. 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