{"corpus_id":215627448,"paper_sha":"f950637276973940aca08accc8508b7ba767be59","doi":"10.1177/0300060519882543","arxiv_id":null,"pmid":32268810,"pmcid":"7153193","mag_id":3015303018,"dblp_id":null,"acl_id":null,"title":"Radiation-resistance increased by overexpression of microRNA-21 and inhibition of its target PTEN in esophageal squamous cell carcinoma","year":2020,"publication_date":"2020-04-01","venue":"Journal of International Medical Research","journal":{"name":"The Journal of International Medical Research","pages":null,"volume":"48"},"journal_issn":null,"journal_title":null,"publication_types":["JournalArticle"],"pubmed_pub_types":["Journal Article"],"s2_fields_of_study":["Medicine"],"reference_count":36,"citation_count":12,"influential_citation_count":0,"is_open_access":true,"arxiv_categories":null,"arxiv_license":null,"arxiv_journal_ref":null,"mesh_headings":[{"d":"Apoptosis","mj":false,"qs":[{"q":"genetics","mj":false,"ui":"Q000235"}],"ui":"D017209"},{"d":"Cell Line, Tumor","mj":false,"ui":"D045744"},{"d":"Cell Proliferation","mj":false,"qs":[{"q":"genetics","mj":false,"ui":"Q000235"}],"ui":"D049109"},{"d":"Esophageal Neoplasms","mj":false,"qs":[{"q":"pathology","mj":false,"ui":"Q000473"}],"ui":"D004938"},{"d":"Esophageal Squamous Cell Carcinoma","mj":false,"qs":[{"q":"genetics","mj":true,"ui":"Q000235"},{"q":"metabolism","mj":false,"ui":"Q000378"}],"ui":"D000077277"},{"d":"Gene Expression Regulation, Neoplastic","mj":false,"qs":[{"q":"genetics","mj":false,"ui":"Q000235"}],"ui":"D015972"},{"d":"Humans","mj":false,"ui":"D006801"},{"d":"MicroRNAs","mj":false,"qs":[{"q":"genetics","mj":true,"ui":"Q000235"},{"q":"metabolism","mj":false,"ui":"Q000378"},{"q":"radiation effects","mj":false,"ui":"Q000528"}],"ui":"D035683"},{"d":"PTEN Phosphohydrolase","mj":false,"qs":[{"q":"genetics","mj":false,"ui":"Q000235"},{"q":"metabolism","mj":false,"ui":"Q000378"},{"q":"radiation effects","mj":false,"ui":"Q000528"}],"ui":"D051059"},{"d":"Radiation","mj":false,"ui":"D011827"},{"d":"Radiation Tolerance","mj":false,"qs":[{"q":"genetics","mj":true,"ui":"Q000235"}],"ui":"D011836"},{"d":"Radiotherapy","mj":false,"qs":[{"q":"methods","mj":false,"ui":"Q000379"}],"ui":"D011878"}],"chemicals":[{"n":"MIRN21 microRNA, human","ui":"C524940","reg":"0"},{"n":"MicroRNAs","ui":"D035683","reg":"0"},{"n":"PTEN Phosphohydrolase","ui":"D051059","reg":"EC 3.1.3.67"},{"n":"PTEN protein, human","ui":"C494929","reg":"EC 3.1.3.67"}],"comments_corrections":null,"source_flags":5,"s2_open_access_pdf_url":"https://journals.sagepub.com/doi/pdf/10.1177/0300060519882543","s2_open_access_landing_url":"https://www.semanticscholar.org/paper/f950637276973940aca08accc8508b7ba767be59","s2_open_access_license":"CCBYNC","s2_open_access_status":"GOLD","pmc_open_access_pdf_url":null,"pmc_open_access_landing_url":null,"pmc_open_access_license":null,"pmc_open_access_status":null,"unpaywall_open_access_pdf_url":null,"unpaywall_open_access_landing_url":null,"unpaywall_open_access_license":null,"unpaywall_open_access_status":null,"abstract":"Objective Overexpression of microRNA-21 (miR-21) increases the radiation resistance of esophageal squamous cell carcinoma (ESCC). However, the molecular mechanism responsible for this action is still unclear. In the present study, we investigated the role of phosphatase and tensin homolog deleted on chromosome 10 (PTEN) in miR-21-enhanced radiation resistance in patients with ESCC. Methods We evaluated the association between miR-21 levels and radiation resistance in patients with ESCC. We also investigated the role of PTEN in the proliferation and apoptosis of ESCC cells transfected with miR-21 inhibitor during irradiation, using PTEN small interfering RNA (siRNA). Results MiR-21 levels were significantly higher in radiation-resistant patients. Downregulation of miR-21 during irradiation suppressed the radiation resistance of ESCC cells, demonstrated by decreased cell proliferation and increased cell apoptosis. PTEN siRNA attenuated miR-21-induced suppression of radiation resistance in ESCC cells. Conclusions These results suggest that miR-21 enhanced the radiation resistance of ESCC by inhibiting PTEN. MiR-21 and PTEN are potential therapeutic biotargets for ESCC.","claims":[{"public_id":"cl_d88167a09265d73b015a4b2c3d2b08e2","status":"active","text":"Downregulation of microRNA-21 during irradiation suppresses radiation resistance in esophageal squamous cell carcinoma cells, with decreased proliferation and increased apoptosis.","confidence":0.97,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_d88167a09265d73b015a4b2c3d2b08e2"},{"public_id":"cl_68c649ab5b2edd950a50fd8f7bcd2949","status":"active","text":"MiR-21 levels are significantly higher in radiation-resistant patients with esophageal squamous cell carcinoma.","confidence":0.98,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous 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