{"corpus_id":216509870,"paper_sha":"bf5ccaf0e9ad2fe8bb96f1a502825f91e144da83","doi":"10.1016/j.lssr.2020.02.001","arxiv_id":null,"pmid":32718685,"pmcid":null,"mag_id":3010289827,"dblp_id":null,"acl_id":null,"title":"Proteomic analysis in different development stages on SP0 generation of rice seeds after space flight.","year":2020,"publication_date":"2020-08-01","venue":"Life sciences and space research","journal":{"name":"Life sciences in space research","pages":"\n          34-45\n        ","volume":"26"},"journal_issn":null,"journal_title":null,"publication_types":["JournalArticle"],"pubmed_pub_types":["Journal Article"],"s2_fields_of_study":["Biology","Medicine","Environmental Science"],"reference_count":50,"citation_count":21,"influential_citation_count":0,"is_open_access":false,"arxiv_categories":null,"arxiv_license":null,"arxiv_journal_ref":null,"mesh_headings":[{"d":"Germination","mj":true,"ui":"D018525"},{"d":"Oryza","mj":false,"qs":[{"q":"growth & development","mj":true,"ui":"Q000254"}],"ui":"D012275"},{"d":"Plant Proteins","mj":false,"qs":[{"q":"metabolism","mj":true,"ui":"Q000378"}],"ui":"D010940"},{"d":"Proteome","mj":false,"qs":[{"q":"metabolism","mj":true,"ui":"Q000378"}],"ui":"D020543"},{"d":"Proteomics","mj":false,"ui":"D040901"},{"d":"Seeds","mj":false,"qs":[{"q":"growth & development","mj":true,"ui":"Q000254"}],"ui":"D012639"},{"d":"Space Flight","mj":true,"ui":"D013026"}],"chemicals":[{"n":"Plant Proteins","ui":"D010940","reg":"0"},{"n":"Proteome","ui":"D020543","reg":"0"}],"comments_corrections":null,"source_flags":5,"s2_open_access_pdf_url":null,"s2_open_access_landing_url":null,"s2_open_access_license":null,"s2_open_access_status":null,"pmc_open_access_pdf_url":null,"pmc_open_access_landing_url":null,"pmc_open_access_license":null,"pmc_open_access_status":null,"unpaywall_open_access_pdf_url":null,"unpaywall_open_access_landing_url":null,"unpaywall_open_access_license":null,"unpaywall_open_access_status":null,"abstract":"The space biological effects of plants will drive the development of aerospace science and breeding science. The aim of this study is to reveal changes in the proteome of contemporary plants at different growth and development stages after space flight of rice seeds. We carried the rice seeds (DN416) through the SJ-10 returning satellite and returned to the ground for planting to the three-leaf stage (TLP) and tillering stage (TS) after a 12.5-day orbital flight. We found that the space flight caused the rice germination rate, the TLP plant height, and the number of tillers in the TS decreased by 11.64%, 9.75%, and 9.80%, respectively. In addition, the treatment group ROS and MDA level increased in the TLP and TS. The abundance patterns of proteins in these leaves identified 214 proteins in the TLP and 286 in the TS leaves that were markedly changed. Moreover, our study identified D14 proteins that control plant height and tiller. Our results show that the space environment may affect the downstream signaling mechanism by regulating the level of ROS in the body to achieve a response to the space environment. Meanwhile, the space environment may affect the plant height and tiller of rice by altering the expression of D14 protein and hormone-regulated proteins. Our results reveal changes in the proteome of different growth stages of rice plants, and also reveal the molecular mechanism of space environment regulation of rice plant height and tiller, which provides a new direction for further understanding of space biological effects and space mutation breeding.","claims":[{"public_id":"cl_9502ab5d4bb7070aba49d7c2a7d76209","status":"active","text":"D14 proteins were identified as proteins that control plant height and tillering.","confidence":0.93,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_9502ab5d4bb7070aba49d7c2a7d76209"},{"public_id":"cl_307b34b47317969ece7c9dc7f1d896d3","status":"active","text":"Proteomic analysis identified 214 markedly changed proteins in three-leaf-stage leaves and 286 markedly changed proteins in tillering-stage leaves.","confidence":0.98,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_307b34b47317969ece7c9dc7f1d896d3"},{"public_id":"cl_0d53ac6b44014026266460c7f6e640f3","status":"active","text":"ROS and MDA levels increased in both the three-leaf stage and tillering stage after the space-flight treatment.","confidence":0.97,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_0d53ac6b44014026266460c7f6e640f3"},{"public_id":"cl_ed09e54d4eab40706e9b3220737704c4","status":"active","text":"Space flight reduced rice seed germination rate by 11.64%, three-leaf-stage plant height by 9.75%, and tiller number at the tillering stage by 9.80%.","confidence":0.98,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_ed09e54d4eab40706e9b3220737704c4"},{"public_id":"cl_ac3d3d3f591e7d632698332c94c96efb","status":"active","text":"The space environment may regulate rice plant height and tillering by altering D14 protein expression and hormone-regulated proteins, with ROS level changes acting in the downstream signaling response.","confidence":0.9,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_ac3d3d3f591e7d632698332c94c96efb"}],"concepts":[{"public_id":"co_07e8e9dbad0ea009d787b01b5bd8ca14","status":"active","name":"three-leaf stage","description":"An early rice growth stage when plants have three leaves.","types":["growth stage"],"aliases":["TLP"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_07e8e9dbad0ea009d787b01b5bd8ca14"},{"public_id":"co_1cd1cd80dac4d1c799b947e11f1fea8b","status":"active","name":"hormone-regulated proteins","description":"Proteins whose abundance or activity is controlled by hormone signaling.","types":["protein class"],"aliases":[],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_1cd1cd80dac4d1c799b947e11f1fea8b"},{"public_id":"co_296c62434fe233e989de7a9efa5613ce","status":"active","name":"proteomic analysis","description":"The large-scale identification and quantification of proteins in plant leaf samples.","types":["method"],"aliases":[],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_296c62434fe233e989de7a9efa5613ce"},{"public_id":"co_2be28a83fa5744c0f47dab1b44ad1f4d","status":"active","name":"tillering-stage leaves","description":"Leaf samples collected from rice plants at the tillering stage.","types":["sample"],"aliases":[],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_2be28a83fa5744c0f47dab1b44ad1f4d"},{"public_id":"co_55cafaa58ce349e1a3a027c4d637b23d","status":"active","name":"space environment","description":"The conditions encountered by rice seeds during orbital flight.","types":["environment"],"aliases":[],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_55cafaa58ce349e1a3a027c4d637b23d"},{"public_id":"co_79675e32e3f66e76a40c9ebe9d8c0d1d","status":"active","name":"space flight","description":"Orbital exposure of rice seeds during a 12.5-day mission before return and planting on Earth.","types":["treatment","environmental exposure"],"aliases":["space-flight treatment"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_79675e32e3f66e76a40c9ebe9d8c0d1d"},{"public_id":"co_a2bbc11a50794efc3baa27e5e0e71ce0","status":"active","name":"tillering stage","description":"A rice developmental stage characterized by the production of tillers.","types":["growth stage"],"aliases":["TS"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_a2bbc11a50794efc3baa27e5e0e71ce0"},{"public_id":"co_a5566125aca97dfe25d0da58151a60e3","status":"active","name":"reactive oxygen species","description":"Chemically reactive oxygen-containing molecules measured as a stress-related indicator.","types":["biomarker"],"aliases":["ROS"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_a5566125aca97dfe25d0da58151a60e3"},{"public_id":"co_a865803768d36820d8d25aa50933e531","status":"active","name":"three-leaf-stage leaves","description":"Leaf samples collected from rice plants at the three-leaf stage.","types":["sample"],"aliases":[],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_a865803768d36820d8d25aa50933e531"},{"public_id":"co_b113b1ec54f70ee45c6bcaa723c660f6","status":"active","name":"tillering","description":"The formation of additional shoots or tillers in rice.","types":["phenotype"],"aliases":[],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_b113b1ec54f70ee45c6bcaa723c660f6"},{"public_id":"co_bf970e4e5f4213a2654e880e9a29609a","status":"active","name":"D14 proteins","description":"Proteins associated with regulation of plant height and tillering in rice.","types":["protein family"],"aliases":["D14"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_bf970e4e5f4213a2654e880e9a29609a"},{"public_id":"co_cb86537b573e0d0989bcdf721eae2f7b","status":"active","name":"malondialdehyde","description":"A lipid peroxidation product measured as a stress-related indicator.","types":["biomarker"],"aliases":["MDA"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_cb86537b573e0d0989bcdf721eae2f7b"},{"public_id":"co_d5d392ea6f2343781e39b1c809090664","status":"active","name":"plant height","description":"The vertical growth measure of rice plants.","types":["phenotype"],"aliases":[],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_d5d392ea6f2343781e39b1c809090664"},{"public_id":"co_da88dfbf69f463eac012d14e24c260bd","status":"active","name":"rice seed germination rate","description":"The proportion of rice seeds that germinate after treatment.","types":["outcome"],"aliases":[],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_da88dfbf69f463eac012d14e24c260bd"}],"external_ids":{"DOI":"10.1016/j.lssr.2020.02.001","ArXiv":null,"PubMed":32718685,"PubMedCentral":null,"MAG":3010289827,"DBLP":null,"ACL":null},"open_access":{"is_open_access":false,"pdf_url":null,"landing_url":"https://sah.borca.ai/papers/216509870","source":null,"pdf_url_source":null,"license":null,"reason":"pdf_url_not_indexed"},"reference_availability":{"status":"available","references_indexed":true,"full_text_available":false,"full_text_source":null,"count_basis":"semantic_scholar_metadata","extraction_status":"not_applicable","reason":null},"source":{"provider":"episteme2","base_corpus":"semantic_scholar_dump","freshness_mode":"unknown","basis":["semantic_scholar_metadata","postgres_metadata"],"limits":["paper metadata is based on indexed upstream scholarly datasets","claims and concepts are available only for extracted papers","absence of claims or concepts means no extracted graph data is available in this response"],"status":"available","degraded":false,"degraded_reasons":[],"diagnostics":{"status":"available","degraded":false,"degraded_reasons":[],"metadata_status":"available","graph_status":"available","abstract_status":"available"},"source_flags":5},"paper_id":636070,"paper_uid":"f52c290c-b7db-4106-919f-b764893c46c7","canonical_identity":{"paper_id":636070,"paper_uid":"f52c290c-b7db-4106-919f-b764893c46c7","identity_status":"available","lookup_basis":"semantic_scholar_external_id","compatibility_path":"corpus_id"},"url":"https://sah.borca.ai/papers/216509870"}