{"corpus_id":220930733,"paper_sha":"37e4bcc23d2eb0651f6ea5ff996044c9626a4b7d","doi":"10.1186/s12985-020-01390-2","arxiv_id":null,"pmid":32746933,"pmcid":"7397426","mag_id":3046318849,"dblp_id":null,"acl_id":null,"title":"Association of human cytomegalovirus (HCMV) neutralizing antibodies with antibodies to the HCMV glycoprotein complexes","year":2020,"publication_date":"2020-04-27","venue":"Virology Journal","journal":{"name":"Virology Journal","pages":null,"volume":"17"},"journal_issn":null,"journal_title":null,"publication_types":["JournalArticle"],"pubmed_pub_types":["Journal Article","Research Support, Non-U.S. Gov't"],"s2_fields_of_study":["Biology","Medicine"],"reference_count":57,"citation_count":9,"influential_citation_count":0,"is_open_access":true,"arxiv_categories":null,"arxiv_license":null,"arxiv_journal_ref":null,"mesh_headings":[{"d":"Adult","mj":false,"ui":"D000328"},{"d":"Antibodies, Neutralizing","mj":false,"qs":[{"q":"blood","mj":true,"ui":"Q000097"}],"ui":"D057134"},{"d":"Antibodies, Viral","mj":false,"qs":[{"q":"blood","mj":true,"ui":"Q000097"}],"ui":"D000914"},{"d":"Cell Line","mj":false,"ui":"D002460"},{"d":"Cytomegalovirus","mj":false,"qs":[{"q":"genetics","mj":false,"ui":"Q000235"},{"q":"immunology","mj":true,"ui":"Q000276"}],"ui":"D003587"},{"d":"Female","mj":false,"ui":"D005260"},{"d":"Fibroblasts","mj":false,"qs":[{"q":"virology","mj":false,"ui":"Q000821"}],"ui":"D005347"},{"d":"Humans","mj":false,"ui":"D006801"},{"d":"Immunoglobulin G","mj":false,"qs":[{"q":"blood","mj":true,"ui":"Q000097"}],"ui":"D007074"},{"d":"Male","mj":false,"ui":"D008297"},{"d":"Middle Aged","mj":false,"ui":"D008875"},{"d":"Viral Envelope Proteins","mj":false,"qs":[{"q":"immunology","mj":true,"ui":"Q000276"}],"ui":"D014759"},{"d":"Young Adult","mj":false,"ui":"D055815"}],"chemicals":[{"n":"Antibodies, Neutralizing","ui":"D057134","reg":"0"},{"n":"Antibodies, Viral","ui":"D000914","reg":"0"},{"n":"Immunoglobulin G","ui":"D007074","reg":"0"},{"n":"Viral Envelope Proteins","ui":"D014759","reg":"0"}],"comments_corrections":null,"source_flags":5,"s2_open_access_pdf_url":"https://virologyj.biomedcentral.com/track/pdf/10.1186/s12985-020-01390-2","s2_open_access_landing_url":"https://www.semanticscholar.org/paper/37e4bcc23d2eb0651f6ea5ff996044c9626a4b7d","s2_open_access_license":"CCBY","s2_open_access_status":"GOLD","pmc_open_access_pdf_url":null,"pmc_open_access_landing_url":null,"pmc_open_access_license":null,"pmc_open_access_status":null,"unpaywall_open_access_pdf_url":null,"unpaywall_open_access_landing_url":null,"unpaywall_open_access_license":null,"unpaywall_open_access_status":null,"abstract":"Background Human cytomegalovirus (HCMV) causes asymptomatic infections, but also causes congenital infections when women were infected with HCMV during pregnancy, and life-threatening diseases in immunocompromised patients. To better understand the mechanism of the neutralization activity against HCMV, the association of HCMV NT antibody titers was assessed with the antibody titers against each glycoprotein complex (gc) of HCMV. Methods Sera collected from 78 healthy adult volunteers were used. HCMV Merlin strain and HCMV clinical isolate strain 1612 were used in the NT assay with the plaque reduction assay, in which both the MRC-5 fibroblasts cells and the RPE-1 epithelial cells were used. Glycoprotein complex of gB, gH/gL complexes (gH/gL/gO and gH/gL/UL128–131A [PC]) and gM/gN were selected as target glycoproteins. 293FT cells expressed with gB, gM/gN, gH/gL/gO, or PC, were prepared and used for the measurement of the antibody titers against each gc in an indirect immunofluorescence assay (IIFA). The correlation between the IIFA titers to each gc and the HCMV-NT titers was evaluated. Results There were no significant correlations between gB-specific IIFA titers and the HCMV-NT titers in epithelial cells or between gM/gN complex-specific IIFA titers and the HCMV-NT titers. On the other hand, there was a statistically significant positive correlation between the IIFA titers to gH/gL complexes and HCMV-NT titers. Conclusions The data suggest that the gH/gL complexes might be the major target to induce NT activity against HCMV.","claims":[{"public_id":"cl_b0049b55e13f9c2e6de3b02409adffa2","status":"active","text":"No significant correlation was found between gB-specific indirect immunofluorescence assay titers and HCMV neutralization titers in epithelial cells.","confidence":0.98,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_b0049b55e13f9c2e6de3b02409adffa2"},{"public_id":"cl_5d37e6f79fe9b35f8d6d3917a8cd73eb","status":"active","text":"No significant correlation was found between gM/gN complex-specific indirect immunofluorescence assay titers and HCMV neutralization titers.","confidence":0.98,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous 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