{"corpus_id":232200087,"paper_sha":"ce6f7670aacb510ac3f890790949f76f1140a8da","doi":"10.1515/tjb-2020-0129","arxiv_id":null,"pmid":null,"pmcid":null,"mag_id":null,"dblp_id":null,"acl_id":null,"title":"Changes in cell membranes of white blood cells treated with a common food additive E407a","year":2021,"publication_date":"2021-03-12","venue":"Turkish Journal of Biochemistry","journal":{"name":"Turkish Journal of Biochemistry","pages":"557 - 562","volume":"46"},"journal_issn":null,"journal_title":null,"publication_types":[],"pubmed_pub_types":null,"s2_fields_of_study":[],"reference_count":41,"citation_count":5,"influential_citation_count":0,"is_open_access":false,"arxiv_categories":null,"arxiv_license":null,"arxiv_journal_ref":null,"mesh_headings":null,"chemicals":null,"comments_corrections":null,"source_flags":1,"s2_open_access_pdf_url":null,"s2_open_access_landing_url":null,"s2_open_access_license":null,"s2_open_access_status":null,"pmc_open_access_pdf_url":null,"pmc_open_access_landing_url":null,"pmc_open_access_license":null,"pmc_open_access_status":null,"unpaywall_open_access_pdf_url":null,"unpaywall_open_access_landing_url":null,"unpaywall_open_access_license":null,"unpaywall_open_access_status":null,"abstract":"Abstract Objectives To estimate the state of phospholipid bilayer of rats WBCs exposed to a common food additive E407a, which is used as a thickener and emulsifier, during 4 h using a fluorescent probe − ortho-hydroxy derivative of 2,5-diaryl-1,3-oxazole. Materials and methods Steady-state fluorescence spectroscopy: a study by the environment-sensitive fluores-cent probe − 2-(2′-hydroxy-phenyl)-5-phenyl-1,3-oxazole (probe O1O). Results Changes are detected in the spectra of the fluorescent probe bound to rat WBCs treated with the solutions of E407a of various concentrations in comparison with the corresponding spectra of the probe incubated with the untreated leukocytes. The decrease in polarity and proton-donor ability is observed in the lipid membranes of leukocytes in the region, where the probe locates. Conclusions Our findings suggest a higher dehydration of leukocyte membranes of rats treated with a common food additive E407a at high concentrations and, thus, indicate that exposure to high doses of E407a leads to the increase in the lipid order (i.e. to decrease in fluidity) of the membranes of rat WBCs.","claims":[{"public_id":"cl_cb8fb6c4825a3dc03bfbafab14a53428","status":"active","text":"Exposure to high concentrations of E407a decreased polarity and proton-donor ability in the leukocyte lipid membrane region sensed by the probe.","confidence":0.93,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_cb8fb6c4825a3dc03bfbafab14a53428"},{"public_id":"cl_9122189569aa89a19357ce6163b0e494","status":"active","text":"Fluorescence spectra of probe O1O bound to rat white blood cells changed after exposure to E407a at various concentrations compared with untreated leukocytes.","confidence":0.97,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_9122189569aa89a19357ce6163b0e494"},{"public_id":"cl_9874b415e9e234a7945fc7e77eb80e42","status":"active","text":"High doses of E407a increased dehydration and lipid order in rat white blood cell membranes, implying reduced membrane fluidity.","confidence":0.95,"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/claims/cl_9874b415e9e234a7945fc7e77eb80e42"}],"concepts":[{"public_id":"co_0804cf5a36674c561220b543838e1987","status":"active","name":"rat white blood cells","description":"White blood cells from rats used as the biological material in the membrane experiment.","types":["biological sample"],"aliases":["rat WBCs","rat leukocytes","WBCs"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_0804cf5a36674c561220b543838e1987"},{"public_id":"co_20847f84488fc50ce9e6bd6bb1d235e6","status":"active","name":"environment-sensitive fluorescent probe","description":"A probe whose fluorescence depends on local membrane environment properties such as polarity.","types":["measurement probe"],"aliases":["environment-sensitive probe"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_20847f84488fc50ce9e6bd6bb1d235e6"},{"public_id":"co_2ab1dcf491c54740b7c5b79f48359ccd","status":"active","name":"E407a","description":"A common food additive used as a thickener and emulsifier that was applied to rat white blood cells in the experiment.","types":["food additive"],"aliases":["E 407a"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_2ab1dcf491c54740b7c5b79f48359ccd"},{"public_id":"co_35d90568e06472d739f8b55c60d13acb","status":"active","name":"dehydration of leukocyte membranes","description":"A lower hydration state of leukocyte membranes inferred from probe spectral changes.","types":["membrane property"],"aliases":["membrane dehydration"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_35d90568e06472d739f8b55c60d13acb"},{"public_id":"co_4c7221fd1eec1720abf3e06fd51cde6d","status":"active","name":"decrease in fluidity","description":"A reduction in membrane fluidity associated with increased lipid order.","types":["membrane property"],"aliases":["reduced fluidity"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_4c7221fd1eec1720abf3e06fd51cde6d"},{"public_id":"co_597ca607874b12db72155ed7f22facb5","status":"active","name":"lipid order","description":"The degree of ordering of lipid molecules in the membrane.","types":["membrane property"],"aliases":["membrane order"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_597ca607874b12db72155ed7f22facb5"},{"public_id":"co_a2f69dcd0cecf9a191d7b898d43acae4","status":"active","name":"probe O1O","description":"An environment-sensitive fluorescent probe, 2-(2′-hydroxy-phenyl)-5-phenyl-1,3-oxazole, used to sense membrane properties.","types":["fluorescent probe"],"aliases":["2-(2′-hydroxy-phenyl)-5-phenyl-1,3-oxazole","2,5-diaryl-1,3-oxazole derivative","fluorescent probe O1O"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_a2f69dcd0cecf9a191d7b898d43acae4"},{"public_id":"co_a944755fb8e09169beecad1872b3860b","status":"active","name":"phospholipid bilayer","description":"The lipid bilayer structure of the white blood cell membrane assessed in the study.","types":["cell membrane structure"],"aliases":["lipid bilayer"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_a944755fb8e09169beecad1872b3860b"},{"public_id":"co_cba53e22152fa3b0f4b947849f078123","status":"active","name":"lipid membranes of leukocytes","description":"The lipid membrane region of leukocytes where the probe localizes and reports on environmental properties.","types":["cell membrane"],"aliases":["leukocyte lipid membranes"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_cba53e22152fa3b0f4b947849f078123"},{"public_id":"co_f7af36a0fa8e8baaf31c7f50bb95d13e","status":"active","name":"steady-state fluorescence spectroscopy","description":"A fluorescence measurement method used to record probe spectra under different exposure conditions.","types":["measurement technique"],"aliases":["fluorescence spectroscopy"],"contributors":[{"id":1,"public_id":"12632b8b5f","public_label":"Anonymous (12632b8b5f)","roles":["extraction"],"url":"https://sah.borca.ai/u/12632b8b5f"}],"url":"https://sah.borca.ai/concepts/co_f7af36a0fa8e8baaf31c7f50bb95d13e"}],"external_ids":{"DOI":"10.1515/tjb-2020-0129","ArXiv":null,"PubMed":null,"PubMedCentral":null,"MAG":null,"DBLP":null,"ACL":null},"open_access":{"is_open_access":false,"pdf_url":null,"landing_url":"https://sah.borca.ai/papers/232200087","source":null,"pdf_url_source":null,"license":null,"reason":"pdf_url_not_indexed"},"reference_availability":{"status":"available","references_indexed":true,"full_text_available":false,"full_text_source":null,"count_basis":"semantic_scholar_metadata","extraction_status":"not_applicable","reason":null},"source":{"provider":"episteme2","base_corpus":"semantic_scholar_dump","freshness_mode":"unknown","basis":["semantic_scholar_metadata","postgres_metadata"],"limits":["paper metadata is based on indexed upstream scholarly datasets","claims and concepts are available only for extracted papers","absence of claims or concepts means no extracted graph data is available in this response"],"status":"available","degraded":false,"degraded_reasons":[],"diagnostics":{"status":"available","degraded":false,"degraded_reasons":[],"metadata_status":"available","graph_status":"available","abstract_status":"available"},"source_flags":1},"paper_id":631603,"paper_uid":"9e868e1c-870a-4f71-bd89-1986e12c7731","canonical_identity":{"paper_id":631603,"paper_uid":"9e868e1c-870a-4f71-bd89-1986e12c7731","identity_status":"available","lookup_basis":"semantic_scholar_external_id","compatibility_path":"corpus_id"},"url":"https://sah.borca.ai/papers/232200087"}